IDO2 Antibody | Affinity Biosciences

Cites

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Figure 10 Effect of DF supplementation on the activation of the NF-κB signaling pathway.

Product:	IDO2 Antibody
Catalog:	AF0009
Description:	Rabbit polyclonal antibody to IDO2
Application:	ELISA(peptide)
Reactivity:	Human
Mol.Wt.:	47kDa; 47kD(Calculated).
Uniprot:	Q6ZQW0
RRID:	AB_2846779

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

IDO2 Antibody detects endogenous levels of total IDO2.

RRID:

AB_2846779
Cite Format: Affinity Biosciences Cat# AF0009, RRID:AB_2846779.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3-dioxygenase 2; 3-dioxygenase-like protein 1; EC 1.13.11.; I23O2_HUMAN; IDO 2; IDO-2; Ido2; INDOL 1; Indoleamine 2; Indoleamine 2,3 dioxygenase 2; Indoleamine 2,3 dioxygenase like 1 protein; Indoleamine 2,3 dioxygenase like protein 1; Indoleamine pyrrole 2,3 dioxygenase like 1; Indoleamine pyrrole 2,3 dioxygenase like protein 1; Indoleamine-pyrrole 2;

Immunogens

Immunogen:

A synthesized peptide derived from human IDO2

Uniprot:

Q6ZQW0(I23O2_HUMAN) >>Visit HPA database.

Gene(ID):

IDO2(169355)

Expression:

Q6ZQW0 I23O2_HUMAN:

Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon (PubMed:17671174). Also expressed at low level in testis and thyroid. Not expressed in the majority of human tumor samples (>99%) (PubMed:25691885).

Sequence:

Q6ZQW0 I23O2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLHFHYYDTSNKIMEPHRPNVKTAVPLSLESYHISEEYGFLLPDSLKELPDHYRPWMEIANKLPQLIDAHQLQAHVDKMPLLSCQFLKGHREQRLAHLVLSFLTMGYVWQEGEAQPAEVLPRNLALPFVEVSRNLGLPPILVHSDLVLTNWTKKDPDGFLEIGNLETIISFPGGESLHGFILVTALVEKEAVPGIKALVQATNAILQPNQEALLQALQRLRLSIQDITKTLGQMHDYVDPDIFYAGIRIFLSGWKDNPAMPAGLMYEGVSQEPLKYSGGSAAQSTVLHAFDEFLGIRHSKESGDFLYRMRDYMPPSHKAFIEDIHSAPSLRDYILSSGQDHLLTAYNQCVQALAELRSYHITMVTKYLITAAAKAKHGKPNHLPGPPQALKDRGTGGTAVMSFLKSVRDKTLESILHPRG

PTMs - Q6ZQW0 As Substrate

Q6ZQW0

Site	PTM Type	Enzyme	Source
S302	Phosphorylation		Uniprot

Research Backgrounds

Q6ZQW0_I23O2_HUMAN

Function:

Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in immune regulation. May not play a significant role in tryptophan-related tumoral resistance.

Tissue Specificity:

Detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. Also expressed at low level in testis and thyroid. Not expressed in the majority of human tumor samples (>99%).

Family&Domains:

Belongs to the indoleamine 2,3-dioxygenase family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Dietary disodium fumarate supplementation alleviates subacute ruminal acidosis (SARA)-induced liver damage by inhibiting pyroptosis via mitophagy-NLRP3 inflammasome pathway in lactating Hu sheep. Frontiers in Immunology, 2023 (PubMed: 37275885) [IF=7.3]

Application: WB Species: Hu Sheep Sample: liver tissue

Figure 10 Effect of DF supplementation on the activation of the NF-κB signaling pathway. The expression of TLR4-NF-κB pathway-related (A) genes and (B, C) proteins in the liver tissue of Hu sheep was detected. n=6 per group and the values are the mean ± SEM. ** P

2). Chang-Kang-Fang alleviates diarrhea predominant irritable bowel syndrome (IBS-D) through inhibiting TLR4/NF-κB/NLRP3 pathway. Journal of ethnopharmacology, 2024 (PubMed: 38670405) [IF=5.4]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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IDO2 Antibody - #AF0009