SPC25 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562, using SPC25 Antibody.

Product:	SPC25 Antibody
Catalog:	DF13747
Description:	Rabbit polyclonal antibody to SPC25
Application:	WB
Reactivity:	Human
Prediction:	Bovine, Horse, Rabbit, Dog
Mol.Wt.:	26kDa; 26kD(Calculated).
Uniprot:	Q9HBM1
RRID:	AB_2846766

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(88%), Horse(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

SPC25 Antibody detects endogenous levels of total SPC25.

RRID:

AB_2846766
Cite Format: Affinity Biosciences Cat# DF13747, RRID:AB_2846766.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AD024; hSpc25; Kinetochore protein Spc25; SPBC25; SPC25; SPC25_HUMAN; spindle pole body component 25 homolog;

Immunogens

Immunogen:

Uniprot:

Q9HBM1(SPC25_HUMAN) >>Visit HPA database.

Gene(ID):

SPC25(57405)

Sequence:

Q9HBM1 SPC25_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Dog

Rabbit

Pig

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBM1 As Substrate

Q9HBM1

Site	PTM Type	Source
K11	Ubiquitination	Uniprot
S12	Phosphorylation	Uniprot
K19	Ubiquitination	Uniprot
K21	Ubiquitination	Uniprot
K37	Ubiquitination	Uniprot
K41	Ubiquitination	Uniprot
K46	Ubiquitination	Uniprot
S48	Phosphorylation	Uniprot
K50	Ubiquitination	Uniprot
K73	Ubiquitination	Uniprot
K84	Ubiquitination	Uniprot
K90	Ubiquitination	Uniprot
K93	Ubiquitination	Uniprot
K107	Sumoylation	Uniprot
K107	Ubiquitination	Uniprot
K122	Ubiquitination	Uniprot
K133	Ubiquitination	Uniprot
K139	Ubiquitination	Uniprot
K203	Ubiquitination	Uniprot
T204	Phosphorylation	Uniprot

Research Backgrounds

Q9HBM1_SPC25_HUMAN

Function:

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

Subcellular Location:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).

Subunit Structure:

Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C-terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end.

Family&Domains:

Belongs to the SPC25 family.

References

1). SPC25 as a novel therapeutic and prognostic biomarker and its association with glycolysis, ferroptosis and ceRNA in lung adenocarcinoma. Aging (Albany NY), 2024 (PubMed: 38217547)

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SPC25 Antibody - #DF13747