Histone H1.1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Huvec, using Histone H1.

Product:	Histone H1.1 Antibody
Catalog:	DF13743
Description:	Rabbit polyclonal antibody to Histone H1.1
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Horse
Mol.Wt.:	21kDa; 22kD(Calculated).
Uniprot:	Q02539
RRID:	AB_2846762

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Horse(83%)

Clonality:

Polyclonal

Specificity:

Histone H1.1 Antibody detects endogenous levels of total Histone H1.1.

RRID:

AB_2846762
Cite Format: Affinity Biosciences Cat# DF13743, RRID:AB_2846762.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

H1 histone family member 1; H1.1; H1F1; HIST1; HIST1H1A; Histone 1 H1a; Histone cluster 1 H1a;

Immunogens

Immunogen:

Uniprot:

Q02539(H11_HUMAN) >>Visit HPA database.

Gene(ID):

HIST1H1A(3024)

Sequence:

Q02539 H11_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSETVPPAPAASAAPEKPLAGKKAKKPAKAAAASKKKPAGPSVSELIVQAASSSKERGGVSLAALKKALAAAGYDVEKNNSRIKLGIKSLVSKGTLVQTKGTGASGSFKLNKKASSVETKPGASKVATKTKATGASKKLKKATGASKKSVKTPKKAKKPAATRKSSKNPKKPKTVKPKKVAKSPAKAKAVKPKAAKARVTKPKTAKPKKAAPKKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02539 As Substrate

Q02539

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T4	Phosphorylation	P06493 (CDK1)	Uniprot
K17	Ubiquitination		Uniprot
K26	Acetylation		Uniprot
K55	Ubiquitination		Uniprot
S61	Phosphorylation		Uniprot
K67	Sumoylation		Uniprot
K67	Ubiquitination		Uniprot
Y74	Phosphorylation		Uniprot
K78	Acetylation		Uniprot
K78	Ubiquitination		Uniprot
S81	Phosphorylation		Uniprot
K88	Acetylation		Uniprot
K88	Ubiquitination		Uniprot
S89	Phosphorylation		Uniprot
S92	Phosphorylation		Uniprot
K93	Acetylation		Uniprot
K93	Ubiquitination		Uniprot
T95	Phosphorylation		Uniprot
K100	Ubiquitination		Uniprot
T102	Phosphorylation		Uniprot
S105	Phosphorylation		Uniprot
S107	Phosphorylation		Uniprot
K109	Acetylation		Uniprot
K109	Ubiquitination		Uniprot
K112	Ubiquitination		Uniprot
K120	Ubiquitination		Uniprot
K125	Ubiquitination		Uniprot
T152	Phosphorylation	P24941 (CDK2)	Uniprot
T162	Phosphorylation		Uniprot
S165	Phosphorylation		Uniprot
S166	Phosphorylation		Uniprot
S183	Phosphorylation	Q15369 (ELOC) , P50750 (CDK9)	Uniprot
T204	Phosphorylation		Uniprot

Research Backgrounds

Q02539_H11_HUMAN

Function:

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

PTMs:

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.

Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

Subcellular Location:

Nucleus. Chromosome.
Note: Mainly localizes in euchromatin.

Subunit Structure:

Interacts with DFFB.

Family&Domains:

The C-terminal domain is required for high-affinity binding to chromatin.

Belongs to the histone H1/H5 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Histone H1.1 Antibody - #DF13743