PAG Antibody - #DF13738

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Product: PAG Antibody
Catalog: DF13738
Description: Rabbit polyclonal antibody to PAG
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 46kDa; 47kD(Calculated).
Uniprot: Q9NWQ8
RRID: AB_2846757

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PAG Antibody detects endogenous levels of total PAG.
RRID:
AB_2846757
Cite Format: Affinity Biosciences Cat# DF13738, RRID:AB_2846757.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CBP; Csk binding protein; Csk-binding protein; FLJ37858; MGC138364; PAG 1; PAG; PAG1; PAG1_HUMAN; Phosphoprotein associated with glycosphingolipid enriched microdomains 1; Phosphoprotein associated with glycosphingolipid enriched microdomains; Phosphoprotein associated with glycosphingolipid microdomains 1; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Protein Associated with Glycosphingolipid Enriched Microdomains; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp;

Immunogens

Immunogen:
Uniprot:

Q9NWQ8(PHAG1_HUMAN) >>Visit HPA database.

Gene(ID):
PAG1(55824)
Expression:
Q9NWQ8 PHAG1_HUMAN:

Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).

Sequence:
MGPAGSLLGSGQMQITLWGSLAAVAIFFVITFLIFLCSSCDREKKPRQHSGDHENLMNVPSDKEMFSRSVTSLATDAPASSEQNGALTNGDILSEDSTLTCMQHYEEVQTSASDLLDSQDSTGKPKCHQSRELPRIPPESAVDTMLTARSVDGDQGLGMEGPYEVLKDSSSQENMVEDCLYETVKEIKEVAAAAHLEKGHSGKAKSTSASKELPGPQTEGKAEFAEYASVDRNKKCRQSVNVESILGNSCDPEEEAPPPVPVKLLDENENLQEKEGGEAEESATDTTSETNKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQLVNKSGQSLTVPESTYTSIQGDPQRSPSSCNDLYATVKDFEKTPNSTLPPAGRPSEEPEPDYEAIQTLNREEEKATLGTNGHHGLVPKENDYESISDLQQGRDITRL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NWQ8 As Substrate

Site PTM Type Enzyme
S50 Phosphorylation
S61 Phosphorylation
K63 Ubiquitination
S67 Phosphorylation
S69 Phosphorylation
S72 Phosphorylation
S80 Phosphorylation
S97 Phosphorylation
T98 Phosphorylation
Y105 Phosphorylation P07948 (LYN)
S150 Phosphorylation
Y163 Phosphorylation
S170 Phosphorylation
S171 Phosphorylation
Y181 Phosphorylation
T183 Phosphorylation
Y227 Phosphorylation
S229 Phosphorylation
S239 Phosphorylation
S288 Phosphorylation
S295 Phosphorylation
S296 Phosphorylation
S298 Phosphorylation
Y299 Phosphorylation
S301 Phosphorylation
T307 Phosphorylation
S314 Phosphorylation
Y317 Phosphorylation P12931 (SRC)
S318 Phosphorylation
S319 Phosphorylation
S339 Phosphorylation
T340 Phosphorylation
Y341 Phosphorylation
T342 Phosphorylation
S343 Phosphorylation
S351 Phosphorylation
S353 Phosphorylation
S354 Phosphorylation
Y359 Phosphorylation
T361 Phosphorylation
S380 Phosphorylation
Y387 Phosphorylation
T392 Phosphorylation
T404 Phosphorylation
Y417 Phosphorylation
S419 Phosphorylation
S421 Phosphorylation

Research Backgrounds

Function:

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.

PTMs:

Palmitoylated.

Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN.

Subcellular Location:

Cell membrane>Single-pass type III membrane protein.
Note: Present in lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).

Subunit Structure:

Interacts with FYN. When phosphorylated, interacts with CSK. Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Interacts with LYN on plasma membrane lipid rafts. Identified in a complex with LYN and STAT3.

Restrictive clause

 

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