TAP1 Antibody - #DF13735
| Product: | TAP1 Antibody |
| Catalog: | DF13735 |
| Description: | Rabbit polyclonal antibody to TAP1 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Monkey |
| Mol.Wt.: | 87kDa; 87kD(Calculated). |
| Uniprot: | Q03518 |
| RRID: | AB_2846754 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13735, RRID:AB_2846754.
Fold/Unfold
ABC 17; ABC transporter MHC 1; ABC17; ABCB 2; ABCB2; Antigen peptide transporter 1; APT 1; APT1; ATP binding cassette sub family B (MDR/TAP) member 2; ATP binding cassette sub family B member 2; ATP binding cassette transporter; ATP-binding cassette sub-family B member 2; D6S114E; FLJ26666; FLJ41500; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF 1; Peptide transporter PSF1; Peptide transporter TAP 1; Peptide transporter TAP1; PSF 1; PSF-1; PSF1; Really interesting new gene 4 protein; RING 4; RING4; TAP 1; TAP1; TAP1*0102N; TAP1_HUMAN; TAP1N; Transporter 1 ATP binding cassette sub family B (MDR/TAP); Transporter 1 ATP binding cassette sub family B; Transporter associated with antigen processing; Transporter ATP binding cassette major histocompatibility complex 1; Y3;
Immunogens
- Q03518 TAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAELLASAGSACSWDFPRAPPSFPPPAASRGGLGGTRSFRPHRGAESPRPGRDRDGVRVPMASSRCPAPRGCRCLPGASLAWLGTVLLLLADWVLLRTALPRIFSLLVPTALPLLRVWAVGLSRWAVLWLGACGVLRATVGSKSENAGAQGWLAALKPLAAALGLALPGLALFRELISWGAPGSADSTRLLHWGSHPTAFVVSYAAALPAAALWHKLGSLWVPGGQGGSGNPVRRLLGCLGSETRRLSLFLVLVVLSSLGEMAIPFFTGRLTDWILQDGSADTFTRNLTLMSILTIASAVLEFVGDGIYNNTMGHVHSHLQGEVFGAVLRQETEFFQQNQTGNIMSRVTEDTSTLSDSLSENLSLFLWYLVRGLCLLGIMLWGSVSLTMVTLITLPLLFLLPKKVGKWYQLLEVQVRESLAKSSQVAIEALSAMPTVRSFANEEGEAQKFREKLQEIKTLNQKEAVAYAVNSWTTSISGMLLKVGILYIGGQLVTSGAVSSGNLVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGKPLPQYEHRYLHRQVAAVGQEPQVFGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAGSQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPERYSRSVLLITQHLSLVEQADHILFLEGGAIREGGTHQQLMEKKGCYWAMVQAPADAPE
PTMs - Q03518 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R30 | Methylation | Uniprot | |
| R37 | Methylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K453 | Ubiquitination | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K537 | Ubiquitination | Uniprot | |
| Y541 | Phosphorylation | Uniprot | |
| T545 | Phosphorylation | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| K666 | Ubiquitination | Uniprot | |
| T694 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: The transmembrane segments seem to form a pore in the membrane.
Heterodimer of TAP1 and TAP2. Interacts with HLA-A*02-B2M complex; this interaction is obligatory for the loading of peptide epitopes delivered to the ER by TAP1-TAP2 transporter. Interacts with PSMB5 and PSMB8. Similarly to classical MHC class I assembly, HLA-E-B2M complex binds TAP1-TAP2 heterodimer and CALR before the peptide loading. Interacts with HLA-F. Interacts with PSMB5 and PSMB8.
(Microbial infection) Interacts with Epstein-Barr virus BNLF2a.
(Microbial infection) Interacts with herpes simplex virus ICP47.
The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
Research Fields
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Membrane transport > ABC transporters.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Immune diseases > Primary immunodeficiency.
· Organismal Systems > Immune system > Antigen processing and presentation. (View pathway)
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