IL21 Receptor Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Mouse spleen, using IL21 Receptor Antibody.

Product:	IL21 Receptor Antibody
Catalog:	DF13734
Description:	Rabbit polyclonal antibody to IL21 Receptor
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig
Mol.Wt.:	59kDa; 59kD(Calculated).
Uniprot:	Q9HBE5
RRID:	AB_2846753

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(88%)

Clonality:

Polyclonal

Specificity:

IL21 Receptor Antibody detects endogenous levels of total IL21 Receptor.

RRID:

AB_2846753
Cite Format: Affinity Biosciences Cat# DF13734, RRID:AB_2846753.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD360; IL 21R; IL-21 receptor; IL-21R; IL21 receptor; IL21R; IL21R_HUMAN; Interleukin 21 receptor; Interleukin-21 receptor; MGC10967; NILR; Novel interleukin receptor;

Immunogens

Immunogen:

Uniprot:

Q9HBE5(IL21R_HUMAN) >>Visit HPA database.

Gene(ID):

IL21R(50615)

Expression:

Q9HBE5 IL21R_HUMAN:

Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.

Sequence:

Q9HBE5 IL21R_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPRGWAAPLLLLLLQGGWGCPDLVCYTDYLQTVICILEMWNLHPSTLTLTWQDQYEELKDEATSCSLHRSAHNATHATYTCHMDVFHFMADDIFSVNITDQSGNYSQECGSFLLAESIKPAPPFNVTVTFSGQYNISWRSDYEDPAFYMLKGKLQYELQYRNRGDPWAVSPRRKLISVDSRSVSLLPLEFRKDSSYELQVRAGPMPGSSYQGTWSEWSDPVIFQTQSEELKEGWNPHLLLLLLLVIVFIPAFWSLKTHPLWRLWKKIWAVPSPERFFMPLYKGCSGDFKKWVGAPFTGSSLELGPWSPEVPSTLEVYSCHPPRSPAKRLQLTELQEPAELVESDGVPKPSFWPTAQNSGGSAYSEERDRPYGLVSIDTVTVLDAEGPCTWPCSCEDDGYPALDLDAGLEPSPGLEDPLLDAGTTVLSCGCVSAGSPGLGGPLGSLLDRLKPPLADGEDWAGGLPWGGRSPGGVSESEAGSPLAGLDMDTFDSGFVGSDCSSPVECDFTSPGDEGPPRSYLRQWVVIPPPLSSPGPQAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HBE5 As Substrate

Q9HBE5

Site	PTM Type	Source
N73	N-Glycosylation	Uniprot
R201	Methylation	Uniprot
W214	C-Glycosylation	Uniprot

Research Backgrounds

Q9HBE5_IL21R_HUMAN

Function:

This is a receptor for interleukin-21.

PTMs:

C-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Tissue Specificity:

Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen.

Subunit Structure:

Heterodimer with the common gamma subunit. Associates with JAK1.

Family&Domains:

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Belongs to the type I cytokine receptor family. Type 4 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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IL21 Receptor Antibody - #DF13734