DERL2 Antibody - #DF13711

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Product: DERL2 Antibody
Catalog: DF13711
Description: Rabbit polyclonal antibody to DERL2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 27kDa; 28kD(Calculated).
Uniprot: Q9GZP9
RRID: AB_2846730

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
DERL2 Antibody detects endogenous levels of total DERL2.
RRID:
AB_2846730
Cite Format: Affinity Biosciences Cat# DF13711, RRID:AB_2846730.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Carcinoma related; CGI 101; Degradation in endoplasmic reticulum protein 2; DER 2; Der1 like domain family member 2; Der1-like protein 2; DER2; DERL 2; DERL2; DERL2_HUMAN; Derlin 2; Derlin-2; Derlin2; DERtrin 2; DERtrin-2; DERtrin2; F LAN 1; F LANa; F-LAN-1; F-LANa; FLANa;

Immunogens

Immunogen:
Uniprot:

Q9GZP9(DERL2_HUMAN) >>Visit HPA database.

Gene(ID):
DERL2(51009)
Expression:
Q9GZP9 DERL2_HUMAN:

Ubiquitous. Overexpressed in various hepatocarcinomas.

Sequence:
MAYQSLRLEYLQIPPVSRAYTTACVLTTAAVQLELITPFQLYFNPELIFKHFQIWRLITNFLFFGPVGFNFLFNMIFLYRYCRMLEEGSFRGRTADFVFMFLFGGFLMTLFGLFVSLVFLGQAFTIMLVYVWSRRNPYVRMNFFGLLNFQAPFLPWVLMGFSLLLGNSIIVDLLGIAVGHIYFFLEDVFPNQPGGIRILKTPSILKAIFDTPDEDPNYNPLPEERPGGFAWGEGQRLGG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
88
Zebrafish
88
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZP9 As Substrate

Site PTM Type Enzyme
K200 Ubiquitination
K206 Ubiquitination
T211 Phosphorylation
Y218 Phosphorylation

Research Backgrounds

Function:

Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal glycoproteins, but not that of misfolded nonglycoproteins. May act by forming a channel that allows the retrotranslocation of misfolded glycoproteins into the cytosol where they are ubiquitinated and degraded by the proteasome. May mediate the interaction between VCP and misfolded glycoproteins. May also be involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation.

(Microbial infection) In contrast to DERL1, it is not involved in the degradation of MHC class I heavy chains following infection by cytomegaloviruses.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Overexpressed in various hepatocarcinomas.

Subunit Structure:

Forms homo- and heterooligomers with DERL3 and, to a lesser extent, with DERL1. Interacts with the SEL1L/SYVN1 and VCP/SELENOS protein complexes. Mediates association between VCP and EDEM1, as well as that between VCP and the misfolded glycoproteins. Interacts with OS9. Interacts with SELENOK and SELENOS. Interacts with the signal recognition particle/SRP and the SRP receptor; in the process of endoplasmic reticulum stress-induced pre-emptive quality control. Interacts with CCDC47 (By similarity).

Family&Domains:

Belongs to the derlin family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

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