TBX5 Antibody - #DF13705

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Product: TBX5 Antibody
Catalog: DF13705
Description: Rabbit polyclonal antibody to TBX5
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 57kDa; 58kD(Calculated).
Uniprot: Q99593
RRID: AB_2846724

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(80%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TBX5 Antibody detects endogenous levels of total TBX5.
RRID:
AB_2846724
Cite Format: Affinity Biosciences Cat# DF13705, RRID:AB_2846724.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Holt Oram syndrome; HOS; T box 5; T box protein 5; T box transcription factor TBX 5; T box transcription factor TBX5; T-box protein 5; T-box transcription factor TBX5; TBX 5; TBX5; TBX5_HUMAN; Transcription factor T box 5;

Immunogens

Immunogen:
Uniprot:

Q99593(TBX5_HUMAN) >>Visit HPA database.

Gene(ID):
TBX5(6910)
Sequence:
MADADEGFGLAHTPLEPDAKDLPCDSKPESALGAPSKSPSSPQAAFTQQGMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGKAEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFCTHVFPETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDMELHRMSRMQSKEYPVVPRSTVRQKVASNHSPFSSESRALSTSSNLGSQYQCENGVSGPSQDLLPPPNPYPLPQEHSQIYHCTKRKEEECSTTDHPYKKPYMETSPSEEDSFYRSSYPQQQGLGASYRTESAQRQACMYASSAPPSEPVPSLEDISCNTWPSMPSYSSCTVTTVQPMDRLPYQHFSAHFTSGPLVPRLAGMANHGSPQLGEGMFQHQTSVAHQPVVRQCGPQTGLQSPGTLQPPEFLYSHGVPRTLSPHQYHSVHGVGMVPEWSDNS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
80
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99593 As Substrate

Site PTM Type Enzyme
T13 Phosphorylation
T92 Phosphorylation
Y114 Phosphorylation
Y217 Phosphorylation
S252 Phosphorylation
Y255 Phosphorylation
S261 Phosphorylation
T262 Phosphorylation
K339 Acetylation
Y342 Phosphorylation
S352 Phosphorylation
Y354 Phosphorylation
Y358 Phosphorylation

Research Backgrounds

Function:

DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation. Binds to the core DNA motif of NPPA promoter.

PTMs:

Acetylation at Lys-339 by KAT2A and KAT2B promotes nuclear retention.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Shuttles between the cytoplasm and the nucleus. Acetylation at Lys-339 promotes nuclear retention.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Homodimer (via the T-box); binds DNA as homodimer. Interacts (via the T-box) with NKX2-5 (via the homeobox); this complex binds DNA. Interacts with GATA4. Interacts with KAT2A and KAT2B.

Family&Domains:

The T-Box domain binds to double-stranded DNA (PubMed:26926761).

References

1). Generation of a TBX5 homozygous knockout embryonic stem cell line (WAe009-A-45) by CRISPR/Cas9 editing. Stem Cell Research, 2021 (PubMed: 33497883) [IF=1.2]

Application: WB    Species: Human    Sample: hESC H9

Since TBX5 is mainly expressed in the heart, we verified the deletion of TBX5 by Western blot at the protein level (Fig. 1I). The results showed that TBX5 was completely knocked out in TBX5-KO cardiomyocytes (Fig. 1). Short tandem repeat (STR) analysis showed that TBX5-KO is the same as H9. Table 1 summarizes the characterization of TBX5-KO.

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