SNRNP200 Antibody - #DF13693

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Product: SNRNP200 Antibody
Catalog: DF13693
Description: Rabbit polyclonal antibody to SNRNP200
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 244kDa; 245kD(Calculated).
Uniprot: O75643
RRID: AB_2846712

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SNRNP200 Antibody detects endogenous levels of total SNRNP200.
RRID:
AB_2846712
Cite Format: Affinity Biosciences Cat# DF13693, RRID:AB_2846712.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activating signal cointegrator 1 complex subunit 3-like 1; ASCC3L1; BRR2; BRR2 homolog; HELIC2; RP33; SNRNP200; U5 small nuclear ribonucleoprotein 200 kDa helicase; U5 snRNP-specific 200 kDa protein; U5-200KD; U520_HUMAN;

Immunogens

Immunogen:
Uniprot:

O75643(U520_HUMAN) >>Visit HPA database.

Gene(ID):
SNRNP200(23020)
Expression:
O75643 U520_HUMAN:

Widely expressed.

Sequence:
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTKPQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFIQAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKITDYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTLSANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDRECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFLYQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKTLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPYGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMADMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYITPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYPIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75643 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
Y11 Phosphorylation
Y13 Phosphorylation
K14 Methylation
S17 Phosphorylation
R25 Methylation
S26 Phosphorylation
S42 Phosphorylation
K46 Acetylation
K46 Ubiquitination
Y102 Phosphorylation
K177 Ubiquitination
Y199 Phosphorylation
S207 Phosphorylation
Y217 Phosphorylation
S225 Phosphorylation
K284 Ubiquitination
K285 Ubiquitination
K294 Ubiquitination
K358 Ubiquitination
K368 Ubiquitination
S385 Phosphorylation
T389 Phosphorylation
T393 Phosphorylation
T417 Phosphorylation
K426 Acetylation
K426 Ubiquitination
R427 Methylation
S434 Phosphorylation
S457 Phosphorylation
K466 Ubiquitination
K469 Acetylation
K469 Ubiquitination
K479 Acetylation
K479 Methylation
K479 Ubiquitination
K487 Ubiquitination
T510 Phosphorylation
Y540 Phosphorylation
S546 Phosphorylation
S554 Phosphorylation
K557 Ubiquitination
T606 Phosphorylation
K711 Ubiquitination
K729 Acetylation
T731 Phosphorylation
K745 Ubiquitination
T747 Phosphorylation
S756 Phosphorylation
K770 Ubiquitination
T795 Phosphorylation
K804 Methylation
S835 Phosphorylation
Y922 Phosphorylation
Y924 Phosphorylation
Y926 Phosphorylation
S932 Phosphorylation
K944 Acetylation
K944 Ubiquitination
K966 Ubiquitination
K971 Acetylation
K971 Ubiquitination
K1025 Ubiquitination
K1039 Ubiquitination
K1049 Ubiquitination
S1056 Phosphorylation
S1125 Phosphorylation
K1134 Ubiquitination
K1146 Ubiquitination
T1173 Phosphorylation
K1176 Acetylation
K1176 Ubiquitination
Y1177 Phosphorylation
Y1238 Phosphorylation
K1294 Ubiquitination
Y1321 Phosphorylation
K1404 Ubiquitination
T1409 Phosphorylation
K1417 Ubiquitination
K1421 Ubiquitination
T1428 Phosphorylation
K1431 Ubiquitination
K1443 Sumoylation
S1541 Phosphorylation
K1544 Ubiquitination
K1552 Ubiquitination
K1557 Ubiquitination
T1572 Phosphorylation
K1595 Acetylation
K1595 Ubiquitination
K1603 Ubiquitination
S1625 Phosphorylation
R1648 Methylation
Y1679 Phosphorylation
Y1682 Phosphorylation
K1748 Ubiquitination
Y1754 Phosphorylation
Y1770 Phosphorylation
S1794 Phosphorylation
Y1855 Phosphorylation
K1874 Ubiquitination
K1878 Ubiquitination
K1883 Ubiquitination
T1891 Phosphorylation
S1900 Phosphorylation
S1910 Phosphorylation
S1931 Phosphorylation
S1937 Phosphorylation
S1955 Phosphorylation
S1958 Phosphorylation
Y1959 Phosphorylation
K1961 Ubiquitination
K1972 Ubiquitination
S2002 Phosphorylation
S2020 Phosphorylation
Y2021 Phosphorylation
K2059 Ubiquitination
K2080 Ubiquitination
K2087 Ubiquitination
T2131 Phosphorylation
S2133 Phosphorylation
S2135 Phosphorylation

Research Backgrounds

Function:

Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Involved in spliceosome assembly, activation and disassembly. Mediates changes in the dynamic network of RNA-RNA interactions in the spliceosome. Catalyzes the ATP-dependent unwinding of U4/U6 RNA duplices, an essential step in the assembly of a catalytically active spliceosome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Component of a core complex containing at least PRPF8, SNRNP200, EFTUD2 and SNRNP40. Component of the U5 snRNP and U4/U6-U5 tri-snRNP complexes, building blocks of the spliceosome. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of precatalytic, catalytic and postcatalytic spliceosomal complexes.

Family&Domains:

Contains two helicase domains. The N-terminal helicase domain has catalytic activity by itself, contrary to the C-terminal helicase domain that may have a regulatory role and enhance the activity of the first helicase domain.

Belongs to the helicase family. SKI2 subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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