Product: KIAA0701 Antibody
Catalog: DF13691
Description: Rabbit polyclonal antibody to KIAA0701
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 164kDa; 164kD(Calculated).
Uniprot: A0JNW5
RRID: AB_2846710

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(88%), Sheep(88%)
Clonality:
Polyclonal
Specificity:
KIAA0701 Antibody detects endogenous levels of total KIAA0701.
RRID:
AB_2846710
Cite Format: Affinity Biosciences Cat# DF13691, RRID:AB_2846710.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hypothetical protein LOC23074; UH1BL_HUMAN; UHRF1 binding protein 1 like; UHRF1-binding protein 1-like; UHRF1BP1L; KIAA0701;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAGIIKKQILKHLSRFTKNLSPDKINLSTLKGEGELKNLELDEEVLQNMLDLPTWLAINKVFCNKASIRIPWTKLKTHPICLSLDKVIMEMSTCEEPRSPNGPSPIATASGQSEYGFAEKVVEGISVSVNSIVIRIGAKAFNASFELSQLRIYSVNAHWEHGDLRFTRIQDPQRGEVLTFKEINWQMIRIEADATQSSHLEIMCAPVRLITNQSKIRVTLKRRLKDCNVIATKLVLILDDLLWVLTDSQLKAMVQYAKSLSEAIEKSTEQRKSMAPEPTQSSTVVASAQQVKTTQTSNAPDVNDAIVKLFNDFDVKETSHHLVISHLDLHICDDIHAKEKESNRRITGGAMQLSFTQLTIDYYPYHKAGDSCNHWMYFSDATKTKNGWANELLHEFECNVEMLKQAVKDHNVGSPPKSPTHASPQHTQTEKDYPLKGTCRTPSVLSQQSKAKLMSSSVVVRLADFNIYQVSTAEQCRSSPKSMICCNKKSLYLPQEMSAVYIEFTEYYYPDGKDFPIPSPNLYSQLNALQFTVDERSILWLNQFLLDLKQSLNQFMAVYKLNDNSKSDEHVDVRVDGLMLKFVIPSEVKSECHQDQPRAISIQSSEMIATNTRHCPNCRHSDLEALFQDFKDCDFFSKTYTSFPKSCDNFNLLHPIFQRHAHEQDTKMHEIYKGNITPQLNKNTLKTSAATDVWAVYFSQFWIDYEGMKSGKGRPISFVDSFPLSIWICQPTRYAESQKEPQTCNQVSLNTSQSESSDLAGRLKRKKLLKEYYSTESEPLTNGGQKPSSSDTFFRFSPSSSEADIHLLVHVHKHVSMQINHYQYLLLLFLHESLILLSENLRKDVEAVTGSPASQTSICIGILLRSAELALLLHPVDQANTLKSPVSESVSPVVPDYLPTENGDFLSSKRKQISRDINRIRSVTVNHMSDNRSMSVDLSHIPLKDPLLFKSASDTNLQKGISFMDYLSDKHLGKISEDESSGLVYKSGSGEIGSETSDKKDSFYTDSSSILNYREDSNILSFDSDGNQNILSSTLTSKGNETIESIFKAEDLLPEAASLSENLDISKEETPPVRTLKSQSSLSGKPKERCPPNLAPLCVSYKNMKRSSSQMSLDTISLDSMILEEQLLESDGSDSHMFLEKGNKKNSTTNYRGTAESVNAGANLQNYGETSPDAISTNSEGAQENHDDLMSVVVFKITGVNGEIDIRGEDTEICLQVNQVTPDQLGNISLRHYLCNRPVGSDQKAVIHSKSSPEISLRFESGPGAVIHSLLAEKNGFLQCHIENFSTEFLTSSLMNIQHFLEDETVATVMPMKIQVSNTKINLKDDSPRSSTVSLEPAPVTVHIDHLVVERSDDGSFHIRDSHMLNTGNDLKENVKSDSVLLTSGKYDLKKQRSVTQATQTSPGVPWPSQSANFPEFSFDFTREQLMEENESLKQELAKAKMALAEAHLEKDALLHHIKKMTVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - A0JNW5 As Substrate

Site PTM Type Enzyme
K6 Acetylation
S14 Phosphorylation
K24 Ubiquitination
S67 Phosphorylation
T73 Phosphorylation
S126 Phosphorylation
S131 Phosphorylation
S259 Phosphorylation
S261 Phosphorylation
K266 Ubiquitination
K308 Ubiquitination
Y362 Phosphorylation
Y363 Phosphorylation
Y365 Phosphorylation
S414 Phosphorylation
S418 Phosphorylation
T420 Phosphorylation
S423 Phosphorylation
T427 Phosphorylation
T441 Phosphorylation
S443 Phosphorylation
S446 Phosphorylation
S449 Phosphorylation
K450 Ubiquitination
T612 Phosphorylation
T677 Phosphorylation
S754 Phosphorylation
Y772 Phosphorylation
Y773 Phosphorylation
S774 Phosphorylation
S889 Phosphorylation
S891 Phosphorylation
S933 Phosphorylation
S935 Phosphorylation
S939 Phosphorylation
K950 Methylation
S953 Phosphorylation
T955 Phosphorylation
S962 Phosphorylation
Y966 Phosphorylation
S968 Phosphorylation
S976 Phosphorylation
Y985 Phosphorylation
K986 Ubiquitination
S987 Phosphorylation
S989 Phosphorylation
S1058 Phosphorylation
S1060 Phosphorylation
S1066 Phosphorylation
T1070 Phosphorylation
K1077 Methylation
S1078 Phosphorylation
S1081 Phosphorylation
Y1101 Phosphorylation
S1107 Phosphorylation
S1108 Phosphorylation
S1109 Phosphorylation
K1244 Ubiquitination
S1252 Phosphorylation
S1269 Phosphorylation
S1327 Phosphorylation
T1367 Phosphorylation
S1402 Phosphorylation

Research Backgrounds

Subcellular Location:

Cytoplasm>Cytosol. Early endosome.
Note: Primarily cytosolic. Recruited to early endosomes following STX6 overexpression. Overexpression of both STX6 and UHRF1BP1L results in aberrant tubulation of endosomal membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer. Homodimer (via N-terminus). Associates with the Golgi-associated retrograde protein (GARP) complex. Interacts with GARP complex component VPS52. Interacts (via C-terminal coiled-coil domain) with STX6.

Restrictive clause

 

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For Research Use Only.
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