Chordin Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat brain, using Chordin Antibody.

DF13681 at 1/100 staining Mouse kidney tissue by IHC-P.

DF13681 at 1/100 staining Mouse brain tissue by IHC-P.

DF13681 at 1/100 staining Rat kidney tissue by IHC-P.

DF13681 at 1/100 staining Rat brain tissue by IHC-P.

Product:	Chordin Antibody
Catalog:	DF13681
Description:	Rabbit polyclonal antibody to Chordin
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	102kDa; 102kD(Calculated).
Uniprot:	Q9H2X0
RRID:	AB_2846700

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Chordin Antibody detects endogenous levels of total Chordin.

RRID:

AB_2846700
Cite Format: Affinity Biosciences Cat# DF13681, RRID:AB_2846700.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Chordin; chrd; CHRD_HUMAN; MGC133038; Shortgastrulation protein; SOG;

Immunogens

Immunogen:

Uniprot:

Q9H2X0(CHRD_HUMAN) >>Visit HPA database.

Gene(ID):

CHRD(8646)

Expression:

Q9H2X0 CHRD_HUMAN:

Expressed at the highest level in liver.

Sequence:

Q9H2X0 CHRD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSLPAPPAPLLLLGLLLLGSRPARGAGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQANVSAQEPGFAEVLPNLTVQEMDWLVLGELQMALEWAGRPGLRISGHIAARKSCDVLQSVLCGADALIPVQTGAAGSASLTLLGNGSLIYQVQVVGTSSEVVAMTLETKPQRRDQRTVLCHMAGLQPGGHTAVGICPGLGARGAHMLLQNELFLNVGTKDFPDGELRGHVAALPYCGHSARHDTLPVPLAGALVLPPVKSQAAGHAWLSLDTHCHLHYEVLLAGLGGSEQGTVTAHLLGPPGTPGPRRLLKGFYGSEAQGVVKDLEPELLRHLAKGMASLMITTKGSPRGELRGQVHIANQCEVGGLRLEAAGAEGVRALGAPDTASAAPPVVPGLPALAPAKPGGPGRPRDPNTCFFEGQQRPHGARWAPNYDPLCSLCTCQRRTVICDPVVCPPPSCPHPVQAPDQCCPVCPEKQDVRDLPGLPRSRDPGEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGGTGEVHCEKVQCPRLACAQPVRVNPTDCCKQCPVGSGAHPQLGDPMQADGPRGCRFAGQWFPESQSWHPSVPPFGEMSCITCRCGAGVPHCERDDCSLPLSCGSGKESRCCSRCTAHRRPAPETRTDPELEKEAEGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Zebrafish

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H2X0 As Substrate

Q9H2X0

Site	PTM Type	Source
T91	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
S131	Phosphorylation	Uniprot
S132	Phosphorylation	Uniprot
T942	Phosphorylation	Uniprot

Research Backgrounds

Q9H2X0_CHRD_HUMAN

Function:

Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes (By similarity).

PTMs:

Cleaved by tolloid proteases; cleavage participates in dorsoventral patterning during early development.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed at the highest level in liver.

Subunit Structure:

Interacts with TWSG1 and/or BMP4.

Family&Domains:

Belongs to the chordin family.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

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For Research Use Only.
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Chordin Antibody - #DF13681