Product: CARD12 Antibody
Catalog: DF13673
Description: Rabbit polyclonal antibody to CARD12
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit
Mol.Wt.: 116kDa; 116kD(Calculated).
Uniprot: Q9NPP4
RRID: AB_2846692

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
CARD12 Antibody detects endogenous levels of total CARD12.
RRID:
AB_2846692
Cite Format: Affinity Biosciences Cat# DF13673, RRID:AB_2846692.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

and NACHT-containing protein; CARD 12; CARD; CARD LRR and NACHT containing protein 1; CARD LRR and NACHT containing protein; CARD12; Caspase recruitment domain containing protein 12; Caspase recruitment domain family member 12; Caspase recruitment domain-containing protein 12; CLAN 1; CLAN A; CLAN; CLAN B; CLAN C; CLAN D; Clan protein; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR 2.1; CLR2.1; ICE protease activating factor; Ice protease-activating factor; Ipaf; LRR; NLR family CARD domain containing 4; NLR family CARD domain-containing protein 4; NLRC 4; NLRC4; NLRC4_HUMAN; NOD like receptor C4; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 4; UNQ6189/PRO20215;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9NPP4 NLRC4_HUMAN:

Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

Sequence:
MNFIKDNSRALIQRMGMTVIKQITDDLFVWNVLNREEVNIICCEKVEQDAARGIIHMILKKGSESCNLFLKSLKEWNYPLFQDLNGQSLFHQTSEGDLDDLAQDLKDLYHTPSFLNFYPLGEDIDIIFNLKSTFTEPVLWRKDQHHHRVEQLTLNGLLQALQSPCIIEGESGKGKSTLLQRIAMLWGSGKCKALTKFKFVFFLRLSRAQGGLFETLCDQLLDIPGTIRKQTFMAMLLKLRQRVLFLLDGYNEFKPQNCPEIEALIKENHRFKNMVIVTTTTECLRHIRQFGALTAEVGDMTEDSAQALIREVLIKELAEGLLLQIQKSRCLRNLMKTPLFVVITCAIQMGESEFHSHTQTTLFHTFYDLLIQKNKHKHKGVAASDFIRSLDHCGDLALEGVFSHKFDFELQDVSSVNEDVLLTTGLLCKYTAQRFKPKYKFFHKSFQEYTAGRRLSSLLTSHEPEEVTKGNGYLQKMVSISDITSTYSSLLRYTCGSSVEATRAVMKHLAAVYQHGCLLGLSIAKRPLWRQESLQSVKNTTEQEILKAININSFVECGIHLYQESTSKSALSQEFEAFFQGKSLYINSGNIPDYLFDFFEHLPNCASALDFIKLDFYGGAMASWEKAAEDTGGIHMEEAPETYIPSRAVSLFFNWKQEFRTLEVTLRDFSKLNKQDIRYLGKIFSSATSLRLQIKRCAGVAGSLSLVLSTCKNIYSLMVEASPLTIEDERHITSVTNLKTLSIHDLQNQRLPGGLTDSLGNLKNLTKLIMDNIKMNEEDAIKLAEGLKNLKKMCLFHLTHLSDIGEGMDYIVKSLSSEPCDLEEIQLVSCCLSANAVKILAQNLHNLVKLSILDLSENYLEKDGNEALHELIDRMNVLEQLTALMLPWGCDVQGSLSSLLKHLEEVPQLVKLGLKNWRLTDTEIRILGAFFGKNPLKNFQQLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPDPALVRKLSQVLSKLTFLQEARLVGWQFDDDDLSVITGAFKLVTA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Sheep
100
Bovine
88
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPP4 As Substrate

Site PTM Type Enzyme
K5 Methylation
K315 Acetylation
S533 Phosphorylation Q05655 (PRKCD)
T736 Phosphorylation

Research Backgrounds

Function:

Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria (By similarity).

PTMs:

Phosphorylated at Ser-533 following infection of macrophages with S.typhimurium (Salmonella). Phosphorylation is essential for NLRC4 inflammasome function to promote caspase-1 activation and pyroptosis. PRKCD phosphorylates Ser-533 in vitro.

Subcellular Location:

Cytoplasm. Cytoplasm>Cytosol.
Note: Cytoplasmic filaments.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain.

Subunit Structure:

Homooligomer. Homooligomerizes following activation of Naip proteins by pathogenic proteins such as S.typhimurium (Salmonella) flagellin or PrgJ. Component of the NLRC4 inflammasome, at least composed of NLRC4, caspase-1 (CASP1) and some NAIP family member (By similarity). Interacts with ASC, pro-caspase-1, NOD2, BCL10 and NALP1 (NAC) by CARD-CARD interaction. Interacts with EIF2AK2/PKR.

Family&Domains:

In an autoinhibited form the C-terminal leucine-rich repeat (LRR) domain is positioned to sterically occlude one side of the NBD domain and consequently sequester NLRC4 in a monomeric state. An ADP-mediated interaction between the NBD and the WHD also contributes to the autoinhibition.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). Pyroptosis relates to tumor microenvironment remodeling and prognosis: A pan-cancer perspective. Frontiers in Immunology, 2022 (PubMed: 36605187) [IF=7.3]

Application: IHC    Species: Human    Sample:

Figure 8 Expression of selected PRGs in clinical samples. (A) Representative images of expression (brown, cell cytoplasmic/nucleus stain) of GSDMC, GSDMD, GSDME, NLRP3, NLRC4, and IL1B in the clinical samples of colon adenocarcinoma (COAD), head & neck squamous cell carcinoma (HNSC), kidney renal cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC). (B) Expression level (IHC quantification) of PRGs in the clinical samples of colon adenocarcinoma (COAD), head &. Neck squamous cell carcinoma (HNSC), and kidney renal cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC). (C) Differential expression (IHC quantification) of PRGs in the clinical samples of colon adenocarcinoma (COAD), head &. Neck squamous cell carcinoma (HNSC), and kidney renal cell carcinoma (KIRC), liver hepatocellular carcinoma (LIHC), lung adenocarcinoma (LUAD), and lung squamous cell carcinoma (LUSC).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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