PPP2R5C Antibody - #DF13668

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Product: PPP2R5C Antibody
Catalog: DF13668
Description: Rabbit polyclonal antibody to PPP2R5C
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Rabbit
Mol.Wt.: 61kDa; 61kD(Calculated).
Uniprot: Q13362
RRID: AB_2846687

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Rabbit(88%)
Clonality:
Polyclonal
Specificity:
PPP2R5C Antibody detects endogenous levels of total PPP2R5C.
RRID:
AB_2846687
Cite Format: Affinity Biosciences Cat# DF13668, RRID:AB_2846687.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2A5G_HUMAN; B' alpha regulatory subunit; B56G; KIAA0044; MGC23064; PP2A B subunit B' gamma isoform; PP2A B subunit B56 gamma isoform; PP2A B subunit isoform B' gamma; PP2A B subunit isoform B''-gamma; PP2A B subunit isoform B56-gamma; PP2A B subunit isoform PR61-gamma; PP2A B subunit isoform R5-gamma; PP2A B subunit PR61 gamma isoform; PP2A B subunit R5 gamma isoform; Ppp2r5c; PR61G; Protein phosphatase 2 regulatory subunit B (B56) gamma isoform; Protein phosphatase 2 regulatory subunit B' gamma; Protein phosphatase 2, regulatory subunit B', gamma isoform; Renal carcinoma antigen NY REN 29; Renal carcinoma antigen NY-REN-29; Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit gamma isoform; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform;

Immunogens

Immunogen:
Uniprot:

Q13362(2A5G_HUMAN) >>Visit HPA database.

Gene(ID):
PPP2R5C(5527)
Expression:
Q13362 2A5G_HUMAN:

Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.

Sequence:
MLTCNKAGSRMVVDAANSNGPFQPVVLLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKRAALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDSTLTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEKLKMKEREEAWVKIENLAKANPQYTVYSQASTMSIPVAMETDGPLFEDVQMLRKTVKDEAHQAQKDPKKDRPLARRKSELPQDPHTKKALEAHCRADELASQDGR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Horse
75
Bovine
75
Sheep
75
Pig
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13362 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K6 Ubiquitination
S9 Phosphorylation
K45 Ubiquitination
Y79 Phosphorylation
T88 Phosphorylation
K156 Ubiquitination
K183 Ubiquitination
K192 Ubiquitination
Y209 Phosphorylation
K256 Ubiquitination
K258 Ubiquitination
S298 Phosphorylation Q13131 (PRKAA1)
K333 Ubiquitination
S336 Phosphorylation Q13131 (PRKAA1)
S337 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
Y348 Phosphorylation
Y349 Phosphorylation
Y354 Phosphorylation
S373 Phosphorylation
Y375 Phosphorylation
T385 Phosphorylation
K403 Ubiquitination
K413 Ubiquitination
K432 Ubiquitination
K487 Acetylation
S497 Phosphorylation
S520 Phosphorylation Q13315 (ATM)

Research Backgrounds

Function:

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.

PTMs:

Isoform Gamma-3 is phosphorylated on serine residues. Isoform Gamma-1 phosphorylation by ERK2 is IER3-dependent and inhibits ERK dephosphorylation by PP2A-PPP2R5C.

Subcellular Location:

Nucleus. Chromosome>Centromere.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest levels in heart, skeletal muscle and brain. Lower levels in pancreas, kidney, lung and placenta. Very low levels in liver.

Subunit Structure:

PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with PPP2CA AND PPP2R1A; the interaction is direct. Interacts with SGO1; the interaction is direct. Isoform 1 and isoform 2 interact with TP53 (phosphorylated at Ser-15 by ATM); increased upon DNA damage it drives PP2A-mediated dephosphorylation of TP53 at Thr-55. Interacts with IER3 and/or ERK kinases; regulates ERK dephosphorylation. Interacts with CIP2A; this interaction stabilizes CIP2A.

Family&Domains:

Belongs to the phosphatase 2A regulatory subunit B56 family.

Research Fields

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

Restrictive clause

 

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