Rubicon Antibody - #DF13665

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Product: Rubicon Antibody
Catalog: DF13665
Description: Rabbit polyclonal antibody to Rubicon
Application: WB
Reactivity: Human
Prediction: Bovine, Horse, Rabbit, Dog
Mol.Wt.: 108kDa; 109kD(Calculated).
Uniprot: Q92622
RRID: AB_2846684

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(80%), Horse(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
Rubicon Antibody detects endogenous levels of total Rubicon.
RRID:
AB_2846684
Cite Format: Affinity Biosciences Cat# DF13665, RRID:AB_2846684.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Baron; Beclin 1 associated RUN domain containing protein; Beclin-1 associated RUN domain containing protein; hypothetical protein LOC9711; Kiaa0226; OTTHUMP00000208021; OTTHUMP00000208023; OTTHUMP00000208024; RUBIC_HUMAN; Rubicon; RUN domain and cysteine rich domain containing, Beclin 1 interacting protein; Run domain Beclin 1 interacting and cystein rich containing protein; Run domain Beclin-1 interacting and cysteine-rich containing protein; RUN domain protein as Beclin 1 interacting and cysteine rich containing;

Immunogens

Immunogen:
Uniprot:

Q92622(RUBIC_HUMAN) >>Visit HPA database.

Gene(ID):
RUBCN(9711)
Sequence:
MRPEGAGMELGGGEERLPEESRREHWQLLGNLKTTVEGLVSTNSPNVWSKYGGLERLCRDMQSILYHGLIRDQACRRQTDYWQFVKDIRWLSPHSALHVEKFISVHENDQSSADGASERAVAELWLQHSLQYHCLSAQLRPLLGDRQYIRKFYTDAAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKHESPLLVTKSQSLTALPSSTYTPPNSYAQHSYFGSFSSLHQSVPNNGSERRSTSFPLSGPPRKPQESRGHVSPAEDQTIQAPPVSVSALARDSPLTPNEMSSSTLTSPIEASWVSSQNDSPGDASEGPEYLAIGNLDPRGRTASCQSHSSNAESSSSNLFSSSSSQKPDSAASSLGDQEGGGESQLSSVLRRSSFSEGQTLTVTSGAKKSHIRSHSDTSIASRGAPESCNDKAKLRGPLPYSGQSSEVSTPSSLYMEYEGGRYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPMYQEAEHGSFRVTSSSSQFSSRDSAQLSDSGSADEVDEFEIQDADIRRNTASSSKSFVSSQSFSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMAIPSRVLRKWDFSKYYVSNFSKDLLIKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLCHMKNMFKTCRLAKELLDSFDTVPGHLTEDLHLYSLNDLTATRKGELGPRLAELTRAGATHVERCMLCQAKGFICEFCQNEDDIIFPFELHKCRTCEECKACYHKACFKSGSCPRCERLQARREALARQSLESYLSDYEEEPAEALALEAAVLEAT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Dog
88
Rabbit
88
Bovine
80
Pig
75
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92622 As Substrate

Site PTM Type Enzyme
S44 Phosphorylation
K50 Ubiquitination
S92 Phosphorylation
K194 Ubiquitination
S197 Phosphorylation
S246 Phosphorylation
T247 Phosphorylation
S248 Phosphorylation
S266 Phosphorylation
T272 Phosphorylation
S378 Phosphorylation
S387 Phosphorylation
S388 Phosphorylation
S390 Phosphorylation
K403 Ubiquitination
S408 Phosphorylation
S410 Phosphorylation
S416 Phosphorylation
T444 Phosphorylation
Y449 Phosphorylation
Y457 Phosphorylation
S469 Phosphorylation
S473 Phosphorylation
S479 Phosphorylation
S528 Phosphorylation
S562 Phosphorylation
S570 Phosphorylation
S577 Phosphorylation
S581 Phosphorylation
S583 Phosphorylation
S585 Phosphorylation
S609 Phosphorylation
S612 Phosphorylation
S615 Phosphorylation
K660 Ubiquitination
S671 Phosphorylation
K682 Ubiquitination
T709 Phosphorylation
K716 Ubiquitination
T728 Phosphorylation
Y732 Phosphorylation
K734 Ubiquitination
Y772 Phosphorylation
S774 Phosphorylation
S777 Phosphorylation
K778 Ubiquitination
K830 Ubiquitination
S851 Phosphorylation
T856 Phosphorylation
K860 Ubiquitination
K921 Ubiquitination
S952 Phosphorylation

Research Backgrounds

Function:

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation.

Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or DDX58/RIG-I-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes.

Subcellular Location:

Late endosome. Lysosome. Early endosome.
Note: Predominantly located in late endosomes/lysosomes, only partially detected in early endosome and not at all in the Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Associates with PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex II (PI3KC3-C2) in which the core composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 is associated with UVRAG; in the complex interacts directly with PI3KC3 and UVRAG. Interacts with Rab7 (RAB7A or RAB7B) (GTP-bound form); Rab7 and UVRAG compete for RUBCN binding; can interact simultaneously with Rab7 and the PI3K complex. Interacts with CYBA and CYBB; indicative for the association with the CYBA:CYBB NADPH oxidase heterodimer. Interacts with NOX4 and probably associates with the CYBA:NOX4 complex. Interacts with YWHAB and CARD9 in a competetive and stimulation-dependent manner; RUBCN exchanges interaction from YWHAB to CARD9 upon stimulation with beta-1,3-glucan.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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