LOXL4 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from A549, using LOXL4 Antibody.

Product:	LOXL4 Antibody
Catalog:	DF13661
Description:	Rabbit polyclonal antibody to LOXL4
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	84kDa; 84kD(Calculated).
Uniprot:	Q96JB6
RRID:	AB_2846680

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

LOXL4 Antibody detects endogenous levels of total LOXL4.

RRID:

AB_2846680
Cite Format: Affinity Biosciences Cat# DF13661, RRID:AB_2846680.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

4833426I20Rik; FLJ21889; LOX L4; LOXC; LOXL 4; LOXL4; LOXL4_HUMAN; Lysyl oxidase homolog 4; Lysyl oxidase like 4; Lysyl oxidase like protein 4; Lysyl oxidase related C; Lysyl oxidase related protein C; Lysyl oxidase-like protein 4; Lysyl oxidase-related protein C;

Immunogens

Immunogen:

Uniprot:

Q96JB6(LOXL4_HUMAN) >>Visit HPA database.

Gene(ID):

LOXL4(84171)

Expression:

Q96JB6 LOXL4_HUMAN:

Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage.

Sequence:

Q96JB6 LOXL4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAWSPPATLFLFLLLLGQPPPSRPQSLGTTKLRLVGPESKPEEGRLEVLHQGQWGTVCDDNFAIQEATVACRQLGFEAALTWAHSAKYGQGEGPIWLDNVRCVGTESSLDQCGSNGWGVSDCSHSEDVGVICHPRRHRGYLSETVSNALGPQGRRLEEVRLKPILASAKQHSPVTEGAVEVKYEGHWRQVCDQGWTMNNSRVVCGMLGFPSEVPVDSHYYRKVWDLKMRDPKSRLKSLTNKNSFWIHQVTCLGTEPHMANCQVQVAPARGKLRPACPGGMHAVVSCVAGPHFRPPKTKPQRKGSWAEEPRVRLRSGAQVGEGRVEVLMNRQWGTVCDHRWNLISASVVCRQLGFGSAREALFGARLGQGLGPIHLSEVRCRGYERTLSDCPALEGSQNGCQHENDAAVRCNVPNMGFQNQVRLAGGRIPEEGLLEVQVEVNGVPRWGSVCSENWGLTEAMVACRQLGLGFAIHAYKETWFWSGTPRAQEVVMSGVRCSGTELALQQCQRHGPVHCSHGGGRFLAGVSCMDSAPDLVMNAQLVQETAYLEDRPLSQLYCAHEENCLSKSADHMDWPYGYRRLLRFSTQIYNLGRTDFRPKTGRDSWVWHQCHRHYHSIEVFTHYDLLTLNGSKVAEGHKASFCLEDTNCPTGLQRRYACANFGEQGVTVGCWDTYRHDIDCQWVDITDVGPGNYIFQVIVNPHYEVAESDFSNNMLQCRCKYDGHRVWLHNCHTGNSYPANAELSLEQEQRLRNNLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Horse

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96JB6 As Substrate

Q96JB6

Site	PTM Type	Source
S172	Phosphorylation	Uniprot
T175	Phosphorylation	Uniprot
Y183	Phosphorylation	Uniprot
Y383	Phosphorylation	Uniprot
Y475	Phosphorylation	Uniprot

Research Backgrounds

Q96JB6_LOXL4_HUMAN

Function:

May modulate the formation of a collagenous extracellular matrix.

PTMs:

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.

Subcellular Location:

Secreted>Extracellular space.

Tissue Specificity:

Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage.

Family&Domains:

Belongs to the lysyl oxidase family.

References

1). Pathological changes and expression of lysine oxidases and matrix metalloproteinases‐1,‐2, and‐3 in ligaments of patients with haemophilic arthritis. HAEMOPHILIA, 2022 (PubMed: 34697874) [IF=3.9]

Application: WB Species: human Sample: cruciate ligaments

Figure 5.|LOXs and MMP-1,-2,and -3protein expression levels in the cruciate ligaments of patients with OA or HA

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LOXL4 Antibody - #DF13661