Glucose 6 phosphate isomerase Antibody - #DF13660

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Product: Glucose 6 phosphate isomerase Antibody
Catalog: DF13660
Description: Rabbit polyclonal antibody to Glucose 6 phosphate isomerase
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 63kDa; 63kD(Calculated).
Uniprot: P06744
RRID: AB_2846679

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Glucose 6 phosphate isomerase Antibody detects endogenous levels of total Glucose 6 phosphate isomerase.
RRID:
AB_2846679
Cite Format: Affinity Biosciences Cat# DF13660, RRID:AB_2846679.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AMF; Aurocrine motility factor; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; G6PI_HUMAN; Glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; Gpi1; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; NLK; Oxoisomerase; PGI; PHI; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; Phosphosaccharomutase; SA 36; SA-36; SA36; Sperm antigen 36;

Immunogens

Immunogen:
Uniprot:

P06744(G6PI_HUMAN) >>Visit HPA database.

Gene(ID):
GPI(2821)
Sequence:
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06744 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K12 Acetylation
K12 Methylation
K12 Ubiquitination
Y17 Phosphorylation
S22 Phosphorylation
K34 Acetylation
K34 Ubiquitination
Y55 Phosphorylation
S56 Phosphorylation
T61 Phosphorylation
R66 Methylation
K73 Ubiquitination
S74 Phosphorylation
K89 Acetylation
K89 Ubiquitination
Y92 Phosphorylation
T93 Phosphorylation
R106 Methylation
S107 Phosphorylation
T109 Phosphorylation
K116 Acetylation
K116 Ubiquitination
K124 Acetylation
K124 Ubiquitination
K130 Ubiquitination
S131 Phosphorylation
S138 Phosphorylation
K142 Acetylation
K142 Ubiquitination
K147 Ubiquitination
S160 Phosphorylation
T168 Phosphorylation
K172 Ubiquitination
Y174 Phosphorylation
S176 Phosphorylation
Y183 Phosphorylation
S185 Phosphorylation P68400 (CSNK2A1)
K194 Ubiquitination
T195 Phosphorylation
S210 Phosphorylation
K211 Acetylation
K211 Ubiquitination
T214 Phosphorylation
T215 Phosphorylation
K226 Ubiquitination
K234 Acetylation
K234 Ubiquitination
S237 Phosphorylation
K241 Ubiquitination
S247 Phosphorylation
T248 Phosphorylation
T250 Phosphorylation
T251 Phosphorylation
K252 Acetylation
K252 Ubiquitination
S359 Phosphorylation
K362 Acetylation
K362 Ubiquitination
K366 Ubiquitination
C404 S-Nitrosylation
T412 Phosphorylation
K423 Ubiquitination
R438 Methylation
S441 Phosphorylation
K447 Ubiquitination
K454 Acetylation
K454 Ubiquitination
S455 Phosphorylation
K466 Acetylation
K466 Sumoylation
K466 Ubiquitination
K524 Ubiquitination
S532 Phosphorylation

Research Backgrounds

Function:

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.

PTMs:

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.

ISGylated.

Subcellular Location:

Cytoplasm. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; in the catalytically active form. Monomer in the secreted form.

Family&Domains:

Belongs to the GPI family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.

· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

References

1). Novel long non‐coding RNA CYB561‐5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non‐small cell lung cancer. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2022 (PubMed: 35064752) [IF=5.3]

Application: WB    Species: Human    Sample: H1299 cells

FIGURE 4 Lnc‐CYB561‐5 promotes aerobic glycolysis in vivo. (A) Cluster analysis of differentially expressed mRNAs in H1299 cells treated with the lnc‐CYB561‐5 knockdown. (B) The top 20 GO enrichment of significantly upregulated genes (fold change > 2, p < 0.05, top 100). (C) Heatmap depicting relative expression of known genes related to glycolysis. (D and E) Measurement of ECARO and CR in H1299 cells treated with the lnc‐CYB561‐5 knockdown. (F) Relative glycolysis rates in H1299 cells, as judged by Seahorse analyses. (G–I) Relative mRNA and protein expression levels of Pfk1, Hk1, Hk2, Eno1, G6pi and Glut1 in H1299 cells. *p < 0.05, **p < 0.01, ***p < 0.001, NS, p > 0.05 vs. the sh‐Ctrl group, n = 5. Data are presented as means ± SEM. Multiple group comparisons were performed using one‐way ANOVA followed by Tukey's post hoc test

Application: WB    Species: human    Sample: H1299 cells

FIGURE 4| Lnc-CYB561-5 promotes aerobic glycolysis in vivo. (G–I) Relative mRNA and protein expression levels of Pfk1, Hk1, Hk2, Eno1, G6pi and Glut1 in H1299 cells. *p < 0.05, **p < 0.01, ***p < 0.001,NS, p > 0.05 vs. the sh-Ctrl group, n = 5. Data are presented as means ± SEM. Multiple group comparisons were performed using one-way ANOVA followed by Tukey's post hoc test

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