Filaggrin Antibody - #DF13653

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Product: Filaggrin Antibody
Catalog: DF13653
Description: Rabbit polyclonal antibody to Filaggrin
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Mol.Wt.: 30~120kDa; 435kD(Calculated).
Uniprot: P20930
RRID: AB_2846672

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Clonality:
Polyclonal
Specificity:
Filaggrin Antibody detects endogenous levels of total Filaggrin.
RRID:
AB_2846672
Cite Format: Affinity Biosciences Cat# DF13653, RRID:AB_2846672.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATOD2; Epidermal filaggrin; FILA_HUMAN; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin;

Immunogens

Immunogen:
Uniprot:

P20930(FILA_HUMAN) >>Visit HPA database.

Gene(ID):
FLG(2312)
Expression:
P20930 FILA_HUMAN:

Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland (PubMed:19384417). In the skin, mainly expressed in stratum granulosum of the epidermis (PubMed:1429717) (PubMed:19384417).

Sequence:
MSTLLENIFAIINLFKQYSKKDKNTDTLSKKELKELLEKEFRQILKNPDDPDMVDVFMDHLDIDHNKKIDFTEFLLMVFKLAQAYYESTRKENLPISGHKHRKHSHHDKHEDNKQEENKENRKRPSSLERRNNRKGNKGRSKSPRETGGKRHESSSEKKERKGYSPTHREEEYGKNHHNSSKKEKNKTENTRLGDNRKRLSERLEEKEDNEEGVYDYENTGRMTQKWIQSGHIATYYTIQDEAYDTTDSLLEENKIYERSRSSDGKSSSQVNRSRHENTSQVPLQESRTRKRRGSRVSQDRDSEGHSEDSERHSGSASRNHHGSAWEQSRDGSRHPRSHDEDRASHGHSADSSRQSGTRHAETSSRGQTASSHEQARSSPGERHGSGHQQSADSSRHSATGRGQASSAVSDRGHRGSSGSQASDSEGHSENSDTQSVSGHGKAGLRQQSHQESTRGRSGERSGRSGSSLYQVSTHEQPDSAHGRTGTSTGGRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHHEQSVNRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRNEEQSGDGTRHSGSRHHEASSQADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSADSSRKSGTRHTQNSSSGQAASSHEQARSSAGERHGSRHQLQSADSSRHSGTGHGQASSAVRDSGHRGSSGSQATDSEGHSEDSDTQSVSGHGQAGHHQQSHQESARDRSGERSRRSGSFLYQVSTHKQSESSHGWTGPSTGVRQGSHHEQARDNSRHSASQDGQDTIRGHPGSSRRGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSRDGSRHSGSRHHEASSHADISRHSQAGQGQSEGSRTSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSAQEQSRHGSRHPRSHHEDRAGHGHSADSSRQSGTPHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRRQASSAVRDSGHWGSSGSQASDSEGHSEESDTQSVSGHGQDGPHQQSHQESARDWSGGRSGRSGSFIYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRAHPGSRRGGRQGSHHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRQTRKDKQSGDGSRHSGSRHHEAASWADSSRHSQVGQEQSSGSRTSRHQGSSVSQDSDSERHSDDSERLSGSASRNHHGSSREQSRDGSRHPGFHQEDRASHGHSADSSRQSGTHHTESSSHGQAVSSHEQARSSPGERHGSRHQQSADSSRHSGIGHRQASSAVRDSGHRGSSGSQVTNSEGHSEDSDTQSVSAHGQAGPHQQSHKESARGQSGESSGRSRSFLYQVSSHEQSESTHGQTAPSTGGRQGSRHEQARNSSRHSASQDGQDTIRGHPGSSRGGRQGSYHEQSVDRSGHSGYHHSHTTPQGRSDASHGQSGPRSASRQTRNEEQSGDGSRHSGSRHHEPSTRAGSSRHSQVGQGESAGSKTSRRQGSSVSQDRDSEGHSEDSERRSESASRNHYGSAREQSRHGSRNPRSHQEDRASHGHSAESSRQSGTRHAETSSGGQAASSQEQARSSPGERHGSRHQQSADSSTDSGTGRRQDSSVVGDSGNRGSSGSQASDSEGHSEESDTQSVSAHGQAGPHQQSHQESTRGQSGERSGRSGSFLYQVSTHEQSESAHGRTGPSTGGRQRSRHEQARDSSRHSASQEGQDTIRGHPGSSRGGRQGSHYEQSVDSSGHSGSHHSHTTSQERSDVSRGQSGSRSVSRQTRNEKQSGDGSRHSGSRHHEASSRADSSRHSQVGQGQSSGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHLGSAWEQSRDGSRHPGSHHEDRAGHGHSADSSRQSGTRHTESSSRGQAASSHEQARSSAGERHGSHHQLQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDSEGHSEDSDTQSVSAQGKAGPHQQSHKESARGQSGESSGRSGSFLYQVSTHEQSESTHGQSAPSTGGRQGSHYDQAQDSSRHSASQEGQDTIRGHPGPSRGGRQGSHQEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRKTYDKEQSGDGSRHSGSHHHEASSWADSSRHSLVGQGQSSGPRTSRPRGSSVSQDSDSEGHSEDSERRSGSASRNHHGSAQEQSRDGSRHPRSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRTGTSTGGRQGSHHKQARDSSRHSTSQEGQDTIHGHPGSSSGGRQGSHYEQLVDRSGHSGSHHSHTTSQGRSDASHGHSGSRSASRQTRNDEQSGDGSRHSGSRHHEASSRADSSGHSQVGQGQSEGPRTSRNWGSSFSQDSDSQGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQDGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDGSRHSGSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHHGSAQEQLRDGSRHPRSHQEDRAGHGHSADSSRQSGTRHTQTSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGYSGSQASDNEGHSEDSDTQSVSAHGQAGSHQQSHQESARGRSGETSGHSGSFLYQVSTHEQSESSHGWTGPSTRGRQGSRHEQAQDSSRHSASQYGQDTIRGHPGSSRGGRQGYHHEHSVDSSGHSGSHHSHTTSQGRSDASRGQSGSRSASRTTRNEEQSGDSSRHSVSRHHEASTHADISRHSQAVQGQSEGSRRSRRQGSSVSQDSDSEGHSEDSERWSGSASRNHRGSVQEQSRHGSRHPRSHHEDRAGHGHSADRSRQSGTRHAETSSGGQAASSHEQARSSPGERHGSRHQQSADSSRHSGIPRGQASSAVRDSRHWGSSGSQASDSEGHSEESDTQSVSGHGQAGPHQQSHQESARDRSGGRSGRSGSFLYQVSTHEQSESAHGRTRTSTGRRQGSHHEQARDSSRHSASQEGQDTIRGHPGSSRRGRQGSHYEQSVDRSGHSGSHHSHTTSQGRSDASRGQSGSRSASRQTRNDEQSGDGSRHSWSHHHEASTQADSSRHSQSGQGQSAGPRTSRNQGSSVSQDSDSQGHSEDSERWSGSASRNHRGSAQEQSRDGSRHPTSHHEDRAGHGHSAESSRQSGTHHAENSSGGQAASSHEQARSSAGERHGSHHQQSADSSRHSGIGHGQASSAVRDSGHRGSSGSQASDSEGHSEDSDTQSVSAHGQAGPHQQSHQESTRGRSAGRSGRSGSFLYQVSTHEQSESAHGRAGPSTGGRQGSRHEQARDSSRHSASQEGQDTIRGHPGSRRGGRQGSYHEQSVDRSGHSGSHHSHTTSQGRSDASHGQSGSRSASRETRNEEQSGDGSRHSGSRHHEASTQADSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE

PTMs - P20930 As Substrate

Site PTM Type Enzyme
Y18 Phosphorylation
Y85 Phosphorylation
Y86 Phosphorylation
S127 Phosphorylation
T147 Phosphorylation
Y164 Phosphorylation
S165 Phosphorylation
T167 Phosphorylation
Y215 Phosphorylation
Y217 Phosphorylation
Y236 Phosphorylation
S295 Phosphorylation
S303 Phosphorylation
S307 Phosphorylation
S314 Phosphorylation
S316 Phosphorylation
S318 Phosphorylation
R343 Methylation
S345 Phosphorylation
S352 Phosphorylation
S353 Phosphorylation
S432 Phosphorylation
T434 Phosphorylation
Y470 Phosphorylation
T515 Phosphorylation
S535 Phosphorylation
S542 Phosphorylation
S547 Phosphorylation
S562 Phosphorylation
T581 Phosphorylation
S586 Phosphorylation
S622 Phosphorylation
S625 Phosphorylation
S638 Phosphorylation
S640 Phosphorylation
S642 Phosphorylation
S648 Phosphorylation
S702 Phosphorylation
S716 Phosphorylation
Y795 Phosphorylation
S847 Phosphorylation
S848 Phosphorylation
S944 Phosphorylation
S945 Phosphorylation
S950 Phosphorylation
S956 Phosphorylation
S959 Phosphorylation
S982 Phosphorylation
T1012 Phosphorylation
S1013 Phosphorylation
S1014 Phosphorylation
S1020 Phosphorylation
S1021 Phosphorylation
S1055 Phosphorylation
S1114 Phosphorylation
Y1119 Phosphorylation
S1122 Phosphorylation
T1123 Phosphorylation
T1164 Phosphorylation
S1191 Phosphorylation
S1196 Phosphorylation
S1211 Phosphorylation
S1250 Phosphorylation
S1283 Phosphorylation
T1331 Phosphorylation
S1344 Phosphorylation
R1437 Methylation
Y1443 Phosphorylation
S1535 Phosphorylation
T1586 Phosphorylation
S1587 Phosphorylation
S1630 Phosphorylation
S1642 Phosphorylation
S1691 Phosphorylation
S1703 Phosphorylation
S1762 Phosphorylation
Y1767 Phosphorylation
S1770 Phosphorylation
T1771 Phosphorylation
T1812 Phosphorylation
S1827 Phosphorylation
S1894 Phosphorylation
S1895 Phosphorylation
S1919 Phosphorylation
S1922 Phosphorylation
S1954 Phosphorylation
S1999 Phosphorylation
S2013 Phosphorylation
S2034 Phosphorylation
S2047 Phosphorylation
R2086 Methylation
S2087 Phosphorylation
Y2092 Phosphorylation
T2137 Phosphorylation
S2199 Phosphorylation
S2203 Phosphorylation
S2208 Phosphorylation
S2241 Phosphorylation
S2242 Phosphorylation
S2247 Phosphorylation
S2253 Phosphorylation
S2256 Phosphorylation
S2260 Phosphorylation
S2262 Phosphorylation
S2264 Phosphorylation
S2270 Phosphorylation
S2324 Phosphorylation
S2411 Phosphorylation
Y2416 Phosphorylation
S2419 Phosphorylation
T2420 Phosphorylation
S2476 Phosphorylation
S2514 Phosphorylation
T2517 Phosphorylation
R2528 Methylation
S2530 Phosphorylation
S2538 Phosphorylation
S2544 Phosphorylation
T2559 Phosphorylation
S2560 Phosphorylation
S2566 Phosphorylation
S2568 Phosphorylation
S2571 Phosphorylation
S2594 Phosphorylation
S2648 Phosphorylation
S2737 Phosphorylation
Y2740 Phosphorylation
S2851 Phosphorylation
S2854 Phosphorylation
S2856 Phosphorylation
S2889 Phosphorylation
S2890 Phosphorylation
S2895 Phosphorylation
S2901 Phosphorylation
S2904 Phosphorylation
S2918 Phosphorylation
S2972 Phosphorylation
S3061 Phosphorylation
Y3064 Phosphorylation
S3213 Phosphorylation
S3214 Phosphorylation
S3219 Phosphorylation
S3225 Phosphorylation
S3228 Phosphorylation
S3251 Phosphorylation
S3274 Phosphorylation
T3281 Phosphorylation
S3282 Phosphorylation
S3283 Phosphorylation
S3289 Phosphorylation
S3290 Phosphorylation
S3316 Phosphorylation
S3383 Phosphorylation
Y3388 Phosphorylation
S3391 Phosphorylation
T3392 Phosphorylation
T3433 Phosphorylation
S3510 Phosphorylation
T3511 Phosphorylation
S3515 Phosphorylation
S3516 Phosphorylation
S3540 Phosphorylation
S3543 Phosphorylation
S3620 Phosphorylation
S3707 Phosphorylation
Y3712 Phosphorylation
S3715 Phosphorylation
T3716 Phosphorylation
S3722 Phosphorylation
S3737 Phosphorylation
T3757 Phosphorylation
S3784 Phosphorylation
S3789 Phosphorylation
S3804 Phosphorylation
T3835 Phosphorylation
S3843 Phosphorylation
S3850 Phosphorylation
S3853 Phosphorylation
S3861 Phosphorylation
S3915 Phosphorylation
S3938 Phosphorylation
Y3940 Phosphorylation
S3952 Phosphorylation
Y3958 Phosphorylation
Y3984 Phosphorylation
Y4001 Phosphorylation
S4018 Phosphorylation
Y4031 Phosphorylation

Research Backgrounds

Function:

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

PTMs:

Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.

Undergoes deimination of some arginine residues (citrullination).

Subcellular Location:

Cytoplasmic granule.
Note: In the stratum granulosum of the epidermis, localized within keratohyalin granules (PubMed:1429717). In granular keratinocytes and in lower corneocytes, colocalizes with calpain-1/CAPN1 (PubMed:21531719).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal gland. In the skin, mainly expressed in stratum granulosum of the epidermis.

Family&Domains:

Belongs to the S100-fused protein family.

References

1). Therapeutic effects of quinine in a mouse model of atopic dermatitis. Molecular Medicine Reports, 2021 (PubMed: 34240224) [IF=3.4]

Application: WB    Species: Mice    Sample: skin tissue

Figure 4. Effects of quinine on protein and mRNA expression in AD-like mice. (A) Protein expression of FLG and KLK7 evaluated via western blotting. (B) Relative ratio of FLG/GAPDH. (C) Relative ratio of KLK7/GAPDH. (D) Protein expression of FLG evaluated via immunohistochemistry. (E) Protein expression of KLK7 evaluated via immunohistochemistry. mRNA expression of (F) IκBα, (G) IKKα and (H) NF-κB determined via reverse transcription-quantitative PCR analysis. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.001 vs. control; #P<0.05, ##P<0.001 vs. AD. AD, atopic dermatitis; AD + Q, AD group treated with quinine; C + Q, control group treated with quinine; FLG, filaggrin; KLK7, kallikrein-7; IKKα, IκB kinase α.

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