RBM14 Antibody - #DF13645

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Product: RBM14 Antibody
Catalog: DF13645
Description: Rabbit polyclonal antibody to RBM14
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 69kDa; 69kD(Calculated).
Uniprot: Q96PK6
RRID: AB_2846664

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
RBM14 Antibody detects endogenous levels of total RBM14.
RRID:
AB_2846664
Cite Format: Affinity Biosciences Cat# DF13645, RRID:AB_2846664.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CoAA; Coactivator Activator; RBM 14; RBM14; RNA binding motif protein 14; RNA binding protein 14; RRM containing coactivator activator/modulator; SIP; Synaptotagmin interacting protein; SYT interacting protein 1; SYT interacting protein; SYT interacting protein SIP; SYTIP 1; SYTIP1;

Immunogens

Immunogen:
Uniprot:

Q96PK6(RBM14_HUMAN) >>Visit HPA database.

Gene(ID):
RBM14(100526737)
Expression:
Q96PK6 RBM14_HUMAN:

Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.

Sequence:
MKIFVGNVDGADTTPEELAALFAPYGTVMSCAVMKQFAFVHMRENAGALRAIEALHGHELRPGRALVVEMSRPRPLNTWKIFVGNVSAACTSQELRSLFERRGRVIECDVVKDYAFVHMEKEADAKAAIAQLNGKEVKGKRINVELSTKGQKKGPGLAVQSGDKTKKPGAGDTAFPGTGGFSATFDYQQAFGNSTGGFDGQARQPTPPFFGRDRSPLRRSPPRASYVAPLTAQPATYRAQPSVSLGAAYRAQPSASLGVGYRTQPMTAQAASYRAQPSVSLGAPYRGQLASPSSQSAAASSLGPYGGAQPSASALSSYGGQAAAASSLNSYGAQGSSLASYGNQPSSYGAQAASSYGVRAAASSYNTQGAASSLGSYGAQAASYGAQSAASSLAYGAQAASYNAQPSASYNAQSAPYAAQQAASYSSQPAAYVAQPATAAAYASQPAAYAAQATTPMAGSYGAQPVVQTQLNSYGAQASMGLSGSYGAQSAAAATGSYGAAAAYGAQPSATLAAPYRTQSSASLAASYAAQQHPQAAASYRGQPGNAYDGAGQPSAAYLSMSQGAVANANSTPPPYERTRLSPPRASYDDPYKKAVAMSKRYGSDRRLAELSDYRRLSESQLSFRRSPTKSSLDYRRLPDAHSDYARYSGSYNDYLRAAQMHSGYQRRM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
63
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96PK6 As Substrate

Site PTM Type Enzyme
S92 Phosphorylation
K112 Acetylation
K112 Ubiquitination
Y114 Phosphorylation
K121 Ubiquitination
K126 Sumoylation
K126 Ubiquitination
K135 Acetylation
K135 Sumoylation
K135 Ubiquitination
K138 Sumoylation
S147 Phosphorylation
K149 Sumoylation
K149 Ubiquitination
K152 Ubiquitination
K153 Sumoylation
K153 Ubiquitination
S161 Phosphorylation
K164 Acetylation
K164 Ubiquitination
T165 Phosphorylation
K167 Ubiquitination
T206 Phosphorylation
S215 Phosphorylation
S220 Phosphorylation
S225 Phosphorylation
Y226 Phosphorylation
T231 Phosphorylation
T236 Phosphorylation
Y237 Phosphorylation
R238 Methylation
S242 Phosphorylation
S244 O-Glycosylation
S244 Phosphorylation
Y249 Phosphorylation
R250 Methylation
S254 O-Glycosylation
S254 Phosphorylation
S256 O-Glycosylation
S256 Phosphorylation
Y261 Phosphorylation
T263 Phosphorylation
T267 Phosphorylation
S272 Phosphorylation
Y273 Phosphorylation
R274 Methylation
S278 Phosphorylation
S280 O-Glycosylation
S280 Phosphorylation
Y285 Phosphorylation
R286 Methylation
S291 Phosphorylation
Y365 Phosphorylation
T518 Phosphorylation
S520 Phosphorylation
S521 Phosphorylation
S523 Phosphorylation
S527 Phosphorylation
Y528 Phosphorylation
S539 Phosphorylation
Y548 Phosphorylation
S555 Phosphorylation
Y558 Phosphorylation
S560 Phosphorylation
S562 Phosphorylation
S571 Phosphorylation
T572 Phosphorylation
Y576 Phosphorylation
T579 Phosphorylation
S582 Phosphorylation
S587 Phosphorylation
Y588 Phosphorylation
Y592 Phosphorylation
K593 Acetylation
K593 Ubiquitination
K594 Sumoylation
K594 Ubiquitination
K600 Sumoylation
K600 Ubiquitination
S612 Phosphorylation
Y614 Phosphorylation
R615 Methylation
S618 Phosphorylation O14757 (CHEK1)
S620 Phosphorylation
S623 Phosphorylation
S627 Phosphorylation O14757 (CHEK1)
T629 Phosphorylation O14757 (CHEK1)
S631 Phosphorylation
S632 Phosphorylation
Y635 Phosphorylation
S643 Phosphorylation
Y645 Phosphorylation
R647 Methylation
Y648 Phosphorylation
S649 Phosphorylation Q96GD4 (AURKB)
S651 Phosphorylation
Y652 Phosphorylation
Y655 Phosphorylation
R657 Methylation
Y665 Phosphorylation

Research Backgrounds

Function:

Isoform 1 may function as a nuclear receptor coactivator, enhancing transcription through other coactivators such as NCOA6 and CITED1. Isoform 2, functions as a transcriptional repressor, modulating transcriptional activities of coactivators including isoform 1, NCOA6 and CITED1. Regulates centriole biogenesis by suppressing the formation of aberrant centriolar protein complexes in the cytoplasm and thus preserving mitotic spindle integrity. Prevents the formation of the STIL-CENPJ complex (which can induce the formation of aberrant centriolar protein complexes) by interfering with the interaction of STIL with CENPJ. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Cytoplasm.
Note: In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (PubMed:11790299). Cytoplasmic localization is crucial for its function in suppressing the formation of aberrant centriolar protein complexes (PubMed:25385835).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues tested, including brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes.

Subunit Structure:

Isoform 1: Interacts with NCOA6, CITED1 and XRCC5/KU86. Isoform 1: Interacts with SS18 isoform 1. Isoform 1: Interacts with SS18 isoform 2. Interacts with STIL and interferes with its interaction with CENPJ. Interacts with gamma-tubulin.Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA.

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