TIM 4 Antibody | Affinity Biosciences

Product:	TIM 4 Antibody
Catalog:	DF13636
Description:	Rabbit polyclonal antibody to TIM 4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	41kDa; 42kD(Calculated).
Uniprot:	Q96H15
RRID:	AB_2846655

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

TIM 4 Antibody detects endogenous levels of total TIM 4.

RRID:

AB_2846655
Cite Format: Affinity Biosciences Cat# DF13636, RRID:AB_2846655.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

SMUCKLER; Spleen, mucin-containing, knockout of lymphotoxin protein; T cell immunoglobulin and mucin domain containing protein 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing molecule; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin and mucin domains-containing protein 4; T-cell membrane protein 4; TIM-4; Tim4; TIMD 4; TIMD-4; Timd4; TIMD4_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q96H15(TIMD4_HUMAN) >>Visit HPA database.

Gene(ID):

TIMD4(91937)

Sequence:

Q96H15 TIMD4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSKEPLILWLMIEFWWLYLTPVTSETVVTEVLGHRVTLPCLYSSWSHNSNSMCWGKDQCPYSGCKEALIRTDGMRVTSRKSAKYRLQGTIPRGDVSLTILNPSESDSGVYCCRIEVPGWFNDVKINVRLNLQRASTTTHRTATTTTRRTTTTSPTTTRQMTTTPAALPTTVVTTPDLTTGTPLQMTTIAVFTTANTCLSLTPSTLPEEATGLLTPEPSKEGPILTAESETVLPSDSWSSVESTSADTVLLTSKESKVWDLPSTSHVSMWKTSDSVSSPQPGASDTAVPEQNKTTKTGQMDGIPMSMKNEMPISQLLMIIAPSLGFVLFALFVAFLLRGKLMETYCSQKHTRLDYIGDSKNVLNDVQHGREDEDGLFTL

PTMs - Q96H15 As Substrate

Q96H15

Site	PTM Type	Source
T77	Phosphorylation	Uniprot
S135	Phosphorylation	Uniprot
T149	Phosphorylation	Uniprot
Y354	Phosphorylation	Uniprot
S358	Phosphorylation	Uniprot

Research Backgrounds

Q96H15_TIMD4_HUMAN

Function:

Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1 (By similarity).

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Family&Domains:

Recognizes phosphatidyl serine via its immunoglobulin domain.

Belongs to the immunoglobulin superfamily. TIM family.

References

1). Anti-Tim4 Grafting Strongly Hydrophilic Metal–Organic Frameworks Immunoaffinity Flake for High-Efficiency Capture and Separation of Exosomes. ANALYTICAL CHEMISTRY, 2021 (PubMed: 33851819) [IF=7.4]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TIM 4 Antibody - #DF13636