SAP155 Antibody | Affinity Biosciences

Product:	SAP155 Antibody
Catalog:	DF13635
Description:	Rabbit polyclonal antibody to SAP155
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	145kDa; 146kD(Calculated).
Uniprot:	O75533
RRID:	AB_2846654

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SAP155 Antibody detects endogenous levels of total SAP155.

RRID:

AB_2846654
Cite Format: Affinity Biosciences Cat# DF13635, RRID:AB_2846654.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Hsh 155; MDS; Pre mRNA processing 10; Pre mRNA splicing factor SF3b 155 kDa subunit; PRP 10; PRP10; PRPF 10; PRPF10; SAP 155; SAP155; SF3B 1; SF3b155; Spliceosome associated protein 155; Splicing factor 3b subunit 1 155kDa; Splicing factor 3B subunit 1;

Immunogens

Immunogen:

Uniprot:

O75533(SF3B1_HUMAN) >>Visit HPA database.

Gene(ID):

SF3B1(23451)

Sequence:

O75533 SF3B1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75533 As Substrate

O75533

Site	PTM Type	Enzyme	Source
K6	Ubiquitination		Uniprot
K21	Ubiquitination		Uniprot
K22	Ubiquitination		Uniprot
T36	Phosphorylation		Uniprot
Y38	Phosphorylation		Uniprot
Y39	Phosphorylation		Uniprot
Y44	Phosphorylation		Uniprot
T61	Phosphorylation		Uniprot
Y70	Phosphorylation		Uniprot
S72	Phosphorylation		Uniprot
S73	Phosphorylation		Uniprot
S75	Phosphorylation		Uniprot
K81	Ubiquitination		Uniprot
Y84	Phosphorylation		Uniprot
Y101	Phosphorylation		Uniprot
Y119	Phosphorylation		Uniprot
T125	Phosphorylation		Uniprot
S129	Phosphorylation		Uniprot
K141	Acetylation		Uniprot
K141	Ubiquitination		Uniprot
T142	Phosphorylation	P24941 (CDK2)	Uniprot
K146	Ubiquitination		Uniprot
Y152	Phosphorylation		Uniprot
K175	Ubiquitination		Uniprot
K182	Ubiquitination		Uniprot
S190	Phosphorylation		Uniprot
S194	Phosphorylation		Uniprot
K195	Acetylation		Uniprot
K195	Methylation		Uniprot
K195	Ubiquitination		Uniprot
T203	Phosphorylation		Uniprot
T207	Phosphorylation		Uniprot
T211	Phosphorylation	P24941 (CDK2)	Uniprot
K213	Ubiquitination		Uniprot
K214	Sumoylation		Uniprot
K214	Ubiquitination		Uniprot
S216	Phosphorylation		Uniprot
S217	Phosphorylation		Uniprot
T223	Phosphorylation		Uniprot
T227	Phosphorylation		Uniprot
S229	Phosphorylation		Uniprot
R231	Methylation		Uniprot
T235	Phosphorylation		Uniprot
K240	Ubiquitination		Uniprot
S242	Phosphorylation		Uniprot
T244	Phosphorylation	P24941 (CDK2)	Uniprot
T248	Phosphorylation	P24941 (CDK2)	Uniprot
S251	Phosphorylation		Uniprot
T257	Phosphorylation		Uniprot
S259	Phosphorylation	P24941 (CDK2)	Uniprot
T261	Phosphorylation	P24941 (CDK2)	Uniprot
T267	Phosphorylation	P24941 (CDK2)	Uniprot
T273	Phosphorylation	Q13627 (DYRK1A)	Uniprot
T278	Phosphorylation		Uniprot
T285	Phosphorylation		Uniprot
S286	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
T296	Phosphorylation		Uniprot
T299	Phosphorylation		Uniprot
T303	Phosphorylation		Uniprot
S308	Phosphorylation		Uniprot
T313	Phosphorylation	P24941 (CDK2)	Uniprot
T316	Phosphorylation		Uniprot
R318	Methylation		Uniprot
S322	Phosphorylation		Uniprot
T326	Phosphorylation		Uniprot
T328	Phosphorylation		Uniprot
S332	Phosphorylation		Uniprot
K333	Acetylation		Uniprot
K333	Ubiquitination		Uniprot
T341	Phosphorylation		Uniprot
S344	Phosphorylation		Uniprot
S349	Phosphorylation		Uniprot
T350	Phosphorylation		Uniprot
T354	Phosphorylation	P24941 (CDK2)	Uniprot
T362	Phosphorylation		Uniprot
T369	Phosphorylation		Uniprot
T371	Phosphorylation		Uniprot
S377	Phosphorylation		Uniprot
T379	Phosphorylation		Uniprot
S400	Phosphorylation		Uniprot
K413	Sumoylation		Uniprot
K413	Ubiquitination		Uniprot
Y421	Phosphorylation		Uniprot
T426	Phosphorylation	P24941 (CDK2)	Uniprot
T432	Phosphorylation	P24941 (CDK2)	Uniprot
T434	Phosphorylation	Q9Y463 (DYRK1B) , P24941 (CDK2) , Q13627 (DYRK1A)	Uniprot
T436	Phosphorylation	P24941 (CDK2)	Uniprot
T442	Phosphorylation		Uniprot
K454	Ubiquitination		Uniprot
K468	Sumoylation		Uniprot
K468	Ubiquitination		Uniprot
Y474	Phosphorylation		Uniprot
K477	Ubiquitination		Uniprot
S488	Phosphorylation		Uniprot
K493	Ubiquitination		Uniprot
K499	Sumoylation		Uniprot
K499	Ubiquitination		Uniprot
T508	Phosphorylation		Uniprot
R517	Methylation		Uniprot
S541	Phosphorylation		Uniprot
K554	Acetylation		Uniprot
K554	Ubiquitination		Uniprot
R558	Methylation		Uniprot
K562	Acetylation		Uniprot
K700	Ubiquitination		Uniprot
K733	Ubiquitination		Uniprot
Y761	Phosphorylation		Uniprot
Y764	Phosphorylation		Uniprot
K785	Ubiquitination		Uniprot
K790	Ubiquitination		Uniprot
K793	Ubiquitination		Uniprot
T808	Phosphorylation		Uniprot
K816	Ubiquitination		Uniprot
S851	Phosphorylation		Uniprot
K858	Ubiquitination		Uniprot
K866	Ubiquitination		Uniprot
K874	Ubiquitination		Uniprot
K926	Ubiquitination		Uniprot
K963	Ubiquitination		Uniprot
K1008	Ubiquitination		Uniprot
K1014	Ubiquitination		Uniprot
T1021	Phosphorylation		Uniprot
K1025	Ubiquitination		Uniprot
K1030	Ubiquitination		Uniprot
R1045	Methylation		Uniprot
C1059	S-Nitrosylation		Uniprot
K1067	Acetylation		Uniprot
K1102	Ubiquitination		Uniprot
Y1136	Phosphorylation		Uniprot
T1170	Phosphorylation		Uniprot
R1180	Methylation		Uniprot
Y1288	Phosphorylation		Uniprot
K1292	Ubiquitination		Uniprot
Y1295	Phosphorylation		Uniprot
Y1302	Phosphorylation		Uniprot

Research Backgrounds

O75533_SF3B1_HUMAN

Function:

Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

PTMs:

Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.

Citrullinated by PADI4.

Subcellular Location:

Nucleus. Nucleus speckle.
Note: During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.

Subunit Structure:

Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Identified in the spliceosome C complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts directly with the splicing factor U2AF. Within the SF3B complex interacts directly (via HEAT domain) with SF3B3, SF3B5 and (via HEAT domain) with PHF5A. Interacts directly with SF3B6 Ref.36,. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Phosphorylated form interacts with PPP1R8. Interacts with PQBP1. Interacts with RBM17. Interacts with RBM39. Interacts with SETX. Interacts with RBM15.

Family&Domains:

Belongs to the SF3B1 family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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SAP155 Antibody - #DF13635