KDM5C / Jarid1C / SMCX Antibody - #DF13631

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Product: KDM5C / Jarid1C / SMCX Antibody
Catalog: DF13631
Description: Rabbit polyclonal antibody to KDM5C / Jarid1C / SMCX
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 175kDa; 176kD(Calculated).
Uniprot: P41229
RRID: AB_2846650

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
KDM5C / Jarid1C / SMCX Antibody detects endogenous levels of total KDM5C / Jarid1C / SMCX.
RRID:
AB_2846650
Cite Format: Affinity Biosciences Cat# DF13631, RRID:AB_2846650.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DXS1272E; Histone demethylase JARID1C; JARID1C; JmjC domain containing protein SMCX; Jumonji AT rich interactive domain 1C; Jumonji, AT rich interactive domain 1C (RBP2 like); Jumonji/ARID domain-containing protein 1C; KDM5C; KDM5C_HUMAN; Lysine (K) specific demethylase 5C; Lysine-specific demethylase 5C; MRX13; MRXJ; MRXSCJ; MRXSJ; Protein SmcX; Protein Xe169; rbp2 like protein; Selected cDNA on X; SMCX; Smcx homolog X chromosome; SmcX protein; Smcy homolog X linked; XE169; Xe169 protein;

Immunogens

Immunogen:
Uniprot:

P41229(KDM5C_HUMAN) >>Visit HPA database.

Gene(ID):
KDM5C(8242)
Expression:
P41229 KDM5C_HUMAN:

Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.

Sequence:
MEPGSDDFLPPPECPVFEPSWAEFRDPLGYIAKIRPIAEKSGICKIRPPADWQPPFAVEVDNFRFTPRIQRLNELEAQTRVKLNYLDQIAKFWEIQGSSLKIPNVERRILDLYSLSKIVVEEGGYEAICKDRRWARVAQRLNYPPGKNIGSLLRSHYERIVYPYEMYQSGANLVQCNTRPFDNEEKDKEYKPHSIPLRQSVQPSKFNSYGRRAKRLQPDPEPTEEDIEKNPELKKLQIYGAGPKMMGLGLMAKDKTLRKKDKEGPECPPTVVVKEELGGDVKVESTSPKTFLESKEELSHSPEPCTKMTMRLRRNHSNAQFIESYVCRMCSRGDEDDKLLLCDGCDDNYHIFCLLPPLPEIPKGVWRCPKCVMAECKRPPEAFGFEQATREYTLQSFGEMADSFKADYFNMPVHMVPTELVEKEFWRLVNSIEEDVTVEYGADIHSKEFGSGFPVSDSKRHLTPEEEEYATSGWNLNVMPVLEQSVLCHINADISGMKVPWLYVGMVFSAFCWHIEDHWSYSINYLHWGEPKTWYGVPSLAAEHLEEVMKKLTPELFDSQPDLLHQLVTLMNPNTLMSHGVPVVRTNQCAGEFVITFPRAYHSGFNQGYNFAEAVNFCTADWLPAGRQCIEHYRRLRRYCVFSHEELICKMAACPEKLDLNLAAAVHKEMFIMVQEERRLRKALLEKGITEAEREAFELLPDDERQCIKCKTTCFLSALACYDCPDGLVCLSHINDLCKCSSSRQYLRYRYTLDELPAMLHKLKVRAESFDTWANKVRVALEVEDGRKRSLEELRALESEARERRFPNSELLQQLKNCLSEAEACVSRALGLVSGQEAGPHRVAGLQMTLTELRAFLDQMNNLPCAMHQIGDVKGVLEQVEAYQAEAREALASLPSSPGLLQSLLERGRQLGVEVPEAQQLQRQVEQARWLDEVKRTLAPSARRGTLAVMRGLLVAGASVAPSPAVDKAQAELQELLTIAERWEEKAHLCLEARQKHPPATLEAIIREAENIPVHLPNIQALKEALAKARAWIADVDEIQNGDHYPCLDDLEGLVAVGRDLPVGLEELRQLELQVLTAHSWREKASKTFLKKNSCYTLLEVLCPCADAGSDSTKRSRWMEKELGLYKSDTELLGLSAQDLRDPGSVIVAFKEGEQKEKEGILQLRRTNSAKPSPLASSSTASSTTSICVCGQVLAGAGALQCDLCQDWFHGRCVSVPRLLSSPRPNPTSSPLLAWWEWDTKFLCPLCMRSRRPRLETILALLVALQRLPVRLPEGEALQCLTERAISWQGRARQALASEDVTALLGRLAELRQRLQAEPRPEEPPNYPAAPASDPLREGSGKDMPKVQGLLENGDSVTSPEKVAPEEGSGKRDLELLSSLLPQLTGPVLELPEATRAPLEELMMEGDLLEVTLDENHSIWQLLQAGQPPDLERIRTLLELEKAERHGSRARGRALERRRRRKVDRGGEGDDPAREELEPKRVRSSGPEAEEVQEEEELEEETGGEGPPAPIPTTGSPSTQENQNGLEPAEGTTSGPSAPFSTLTPRLHLPCPQQPPQQQL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41229 As Substrate

Site PTM Type Enzyme
K33 Sumoylation
K33 Ubiquitination
K40 Ubiquitination
K45 Ubiquitination
T66 Phosphorylation
K82 Ubiquitination
K91 Ubiquitination
K101 Ubiquitination
K130 Ubiquitination
K188 Ubiquitination
K191 Sumoylation
K191 Ubiquitination
K205 Acetylation
K205 Ubiquitination
K235 Ubiquitination
Y239 Phosphorylation
S287 Phosphorylation
K289 Ubiquitination
K295 Ubiquitination
S299 Phosphorylation
S301 Phosphorylation
C305 S-Nitrosylation
T306 Phosphorylation
K307 Ubiquitination
S317 Phosphorylation
K370 Ubiquitination
K377 Ubiquitination
K459 Ubiquitination
K657 Ubiquitination
K682 Ubiquitination
K687 Ubiquitination
K776 Ubiquitination
S799 Phosphorylation
T849 Phosphorylation
S897 Phosphorylation
K935 Ubiquitination
T946 Phosphorylation
K996 Ubiquitination
K1087 Acetylation
K1092 Ubiquitination
K1114 Ubiquitination
K1121 Ubiquitination
K1127 Ubiquitination
K1151 Ubiquitination
K1156 Ubiquitination
K1241 Acetylation
T1257 Phosphorylation
Y1327 Phosphorylation
S1333 Phosphorylation
S1340 Phosphorylation
S1356 Phosphorylation
T1358 Phosphorylation
S1359 Phosphorylation
K1442 Ubiquitination

Research Backgrounds

Function:

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements. Represses the CLOCK-ARNTL/BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2 (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined. Highest levels found in brain and skeletal muscle.

Subunit Structure:

Part of two distinct complexes, one containing E2F6, and the other containing REST.

Family&Domains:

The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.

Both the JmjC domain and the JmjN domain are required for enzymatic activity.

Belongs to the JARID1 histone demethylase family.

References

1). Comprehensive transcriptomic analyses identify KDM genes-related subtypes with different TME infiltrates in gastric cancer. BMC cancer, 2023 (PubMed: 37202737) [IF=3.8]

Application: WB    Species: Human    Sample: GC

Fig. 2. Experimental validation of functional phenotypes of KDM5C in GC. A Boxplot showed the expression of KDM5C between tumor and normal samples of TCGA-STAD cohort. Statistical difference is identified by t-test, * indicating P value 

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