E1 Ubiquitin Activating Enzyme Antibody

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from A431 cells, using E1 Ubiquitin Activating Enzyme Antibody.

DF13630 at 1/100 staining Human colorectal cancer by IHC-P.

DF13630 at 1/100 staining Human kidney cancer by IHC-P.

DF13630 staining Hela cells by IF/ICC.

Product:	E1 Ubiquitin Activating Enzyme Antibody
Catalog:	DF13630
Description:	Rabbit polyclonal antibody to E1 Ubiquitin Activating Enzyme
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	117kDa; 118kD(Calculated).
Uniprot:	P22314
RRID:	AB_2846649

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

E1 Ubiquitin Activating Enzyme Antibody detects endogenous levels of total E1 Ubiquitin Activating Enzyme.

RRID:

AB_2846649
Cite Format: Affinity Biosciences Cat# DF13630, RRID:AB_2846649.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A1S9; A1S9 protein; A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; CFAP124; CTD-2522E6.1; GXP 1; GXP1; MGC4781; POC20; POC20 centriolar protein homolog; Protein A1S9; SMAX2; Uba1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1_HUMAN; UBA1A; UBE 1; UBE 1X; UBE1; UBE1X; Ubiquitin activating enzyme E1; Ubiquitin-activating enzyme E1; Ubiquitin-like modifier-activating enzyme 1;

Immunogens

Immunogen:

Uniprot:

P22314(UBA1_HUMAN) >>Visit HPA database.

Gene(ID):

UBA1(7317)

Expression:

P22314 UBA1_HUMAN:

Detected in erythrocytes (at protein level). Ubiquitous.

Sequence:

P22314 UBA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22314 As Substrate

P22314

Site	PTM Type	Enzyme	Source
S2	Acetylation		Uniprot
S3	Phosphorylation		Uniprot
S4	Phosphorylation	P06493 (CDK1)	Uniprot
S7	Phosphorylation		Uniprot
S13	Phosphorylation		Uniprot
S21	Phosphorylation		Uniprot
S24	Phosphorylation		Uniprot
S28	Phosphorylation		Uniprot
S31	Phosphorylation		Uniprot
S46	Phosphorylation		Uniprot
Y55	Phosphorylation		Uniprot
S56	Phosphorylation		Uniprot
Y60	Phosphorylation		Uniprot
K68	Acetylation		Uniprot
K68	Ubiquitination		Uniprot
S74	Phosphorylation		Uniprot
R81	Methylation		Uniprot
K89	Acetylation		Uniprot
K89	Ubiquitination		Uniprot
K97	Ubiquitination		Uniprot
R119	Methylation		Uniprot
S140	Phosphorylation		Uniprot
K185	Ubiquitination		Uniprot
T191	Phosphorylation		Uniprot
C234	S-Nitrosylation		Uniprot
Y273	Phosphorylation		Uniprot
T274	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
Y286	Phosphorylation		Uniprot
R288	Methylation		Uniprot
S293	Phosphorylation		Uniprot
K296	Ubiquitination		Uniprot
K304	Methylation		Uniprot
K304	Ubiquitination		Uniprot
S305	Phosphorylation		Uniprot
S309	Phosphorylation		Uniprot
T318	Phosphorylation		Uniprot
K322	Ubiquitination		Uniprot
R368	Methylation		Uniprot
R384	Methylation		Uniprot
K385	Ubiquitination		Uniprot
Y388	Phosphorylation		Uniprot
K411	Ubiquitination		Uniprot
K416	Ubiquitination		Uniprot
K443	Ubiquitination		Uniprot
S460	Phosphorylation		Uniprot
K465	Acetylation		Uniprot
K465	Ubiquitination		Uniprot
K468	Ubiquitination		Uniprot
K470	Acetylation		Uniprot
K470	Ubiquitination		Uniprot
C481	S-Nitrosylation		Uniprot
K526	Ubiquitination		Uniprot
K528	Sumoylation		Uniprot
K528	Ubiquitination		Uniprot
R551	Methylation		Uniprot
R558	Methylation		Uniprot
Y560	Phosphorylation		Uniprot
C588	S-Nitrosylation		Uniprot
Y590	Phosphorylation		Uniprot
K593	Acetylation		Uniprot
K593	Ubiquitination		Uniprot
T600	Phosphorylation		Uniprot
T603	Phosphorylation		Uniprot
K604	Acetylation		Uniprot
K604	Sumoylation		Uniprot
K604	Ubiquitination		Uniprot
T615	Phosphorylation		Uniprot
K627	Acetylation		Uniprot
K627	Ubiquitination		Uniprot
S628	Phosphorylation		Uniprot
C632	S-Nitrosylation		Uniprot
K635	Sumoylation		Uniprot
K635	Ubiquitination		Uniprot
K657	Ubiquitination		Uniprot
K671	Acetylation		Uniprot
K671	Ubiquitination		Uniprot
R678	Methylation		Uniprot
T682	Phosphorylation		Uniprot
K746	Ubiquitination		Uniprot
S781	Phosphorylation		Uniprot
T789	Phosphorylation		Uniprot
S793	Phosphorylation		Uniprot
T800	Phosphorylation		Uniprot
K802	Ubiquitination		Uniprot
S803	Phosphorylation		Uniprot
K806	Ubiquitination		Uniprot
S810	Phosphorylation		Uniprot
S816	Phosphorylation		Uniprot
S820	Phosphorylation		Uniprot
S824	Phosphorylation		Uniprot
K830	Ubiquitination		Uniprot
T832	Phosphorylation		Uniprot
S835	Phosphorylation	P06493 (CDK1)	Uniprot
K838	Acetylation		Uniprot
K838	Ubiquitination		Uniprot
K843	Ubiquitination		Uniprot
S855	Phosphorylation		Uniprot
S866	Phosphorylation		Uniprot
Y873	Phosphorylation		Uniprot
R880	Methylation		Uniprot
K889	Ubiquitination		Uniprot
K923	Ubiquitination		Uniprot
R957	Methylation		Uniprot
Y978	Phosphorylation		Uniprot
K980	Acetylation		Uniprot
K980	Ubiquitination		Uniprot
K1024	Acetylation		Uniprot
C1039	S-Nitrosylation		Uniprot
C1040	S-Nitrosylation		Uniprot
S1044	Phosphorylation		Uniprot

Research Backgrounds

P22314_UBA1_HUMAN

Function:

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.

PTMs:

ISGylated.

Subcellular Location:

Cytoplasm. Mitochondrion. Nucleus.

Nucleus.

Cytoplasm.

Tissue Specificity:

Detected in erythrocytes (at protein level). Ubiquitous.

Subunit Structure:

Monomer (By similarity). Interacts with GAN (via BTB domain).

Family&Domains:

The first 11 amino acids are essential for phosphorylation and exclusive nuclear localization.

Belongs to the ubiquitin-activating E1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

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E1 Ubiquitin Activating Enzyme Antibody - #DF13630