IRF8 Antibody - #DF13627

(3)  
Product: IRF8 Antibody
Catalog: DF13627
Description: Rabbit polyclonal antibody to IRF8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 48kDa; 48kD(Calculated).
Uniprot: Q02556
RRID: AB_2846646

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(88%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
IRF8 Antibody detects endogenous levels of total IRF8.
RRID:
AB_2846646
Cite Format: Affinity Biosciences Cat# DF13627, RRID:AB_2846646.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H ICSBP; H-ICSBP; HGNC:5358; HICSBP; ICSBP 1; ICSBP; ICSBP1; Interferon consensus sequence binding protein 1; Interferon consensus sequence binding protein; Interferon consensus sequence-binding protein; Interferon regulatory factor 8; IRF 8; IRF-8; Irf8; IRF8_HUMAN; MYLS;

Immunogens

Immunogen:
Uniprot:

Q02556(IRF8_HUMAN) >>Visit HPA database.

Gene(ID):
IRF8(3394)
Expression:
Q02556 IRF8_HUMAN:

Predominantly expressed in lymphoid tissues.

Sequence:
MCDRNGGRRLRQWLIEQIDSSMYPGLIWENEEKSMFRIPWKHAGKQDYNQEVDASIFKAWAVFKGKFKEGDKAEPATWKTRLRCALNKSPDFEEVTDRSQLDISEPYKVYRIVPEEEQKCKLGVATAGCVNEVTEMECGRSEIDELIKEPSVDDYMGMIKRSPSPPEACRSQLLPDWWAQQPSTGVPLVTGYTTYDAHHSAFSQMVISFYYGGKLVGQATTTCPEGCRLSLSQPGLPGTKLYGPEGLELVRFPPADAIPSERQRQVTRKLFGHLERGVLLHSSRQGVFVKRLCQGRVFCSGNAVVCKGRPNKLERDEVVQVFDTSQFFRELQQFYNSQGRLPDGRVVLCFGEEFPDMAPLRSKLILVQIEQLYVRQLAEEAGKSCGAGSVMQAPEEPPPDQVFRMFPDICASHQRSFFRENQQITV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Horse
88
Xenopus
71
Chicken
63
Bovine
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02556 As Substrate

Site PTM Type Enzyme
Y107 Phosphorylation
Y110 Phosphorylation
S162 Phosphorylation
S164 Phosphorylation
Y211 Phosphorylation
K240 Ubiquitination
K269 Methylation
Y373 Phosphorylation

Research Backgrounds

Function:

Plays a role as a transcriptional activator or repressor. Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (By similarity). Positively regulates macroautophagy in dendritic cells.

PTMs:

Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (By similarity). Ubiquitination leads to its degradation by the proteasome.

Sumoylated with SUMO3. Desumoylated by SENP1.

Subcellular Location:

Nucleus. Cytoplasm.
Note: In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in lymphoid tissues.

Subunit Structure:

Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with COPS2.

Family&Domains:

Belongs to the IRF family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

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