Product: TFAP4 Antibody
Catalog: DF13624
Description: Rabbit polyclonal antibody to TFAP4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: Q01664
RRID: AB_2846643

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
TFAP4 Antibody detects endogenous levels of total TFAP4.
RRID:
AB_2846643
Cite Format: Affinity Biosciences Cat# DF13624, RRID:AB_2846643.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Activating enhancer binding protein 4; Activating enhancer-binding protein 4; AP 4; AP4; bHLHc41; Class C basic helix-loop-helix protein 41; TFAP 4; TFAP4; TFAP4 protein; TFAP4_HUMAN; Transcription factor AP 4; Transcription factor AP-4; Transcription factor AP4;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01664 As Substrate

Site PTM Type Enzyme
Y3 Phosphorylation
T8 Phosphorylation
K10 Ubiquitination
S13 Phosphorylation
S30 Phosphorylation
T37 Phosphorylation
K103 Ubiquitination
K114 Ubiquitination
S121 Phosphorylation
S123 Phosphorylation
S124 Phosphorylation
K126 Ubiquitination
K133 Ubiquitination
S139 Phosphorylation
K147 Ubiquitination
K187 Ubiquitination
K189 Ubiquitination
K211 Ubiquitination
T282 Phosphorylation
K299 Ubiquitination
S332 Phosphorylation

Research Backgrounds

Function:

Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Homodimer.

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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