TFAP4 Antibody | Affinity Biosciences

Product:	TFAP4 Antibody
Catalog:	DF13624
Description:	Rabbit polyclonal antibody to TFAP4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	39kDa; 39kD(Calculated).
Uniprot:	Q01664
RRID:	AB_2846643

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TFAP4 Antibody detects endogenous levels of total TFAP4.

RRID:

AB_2846643
Cite Format: Affinity Biosciences Cat# DF13624, RRID:AB_2846643.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activating enhancer binding protein 4; Activating enhancer-binding protein 4; AP 4; AP4; bHLHc41; Class C basic helix-loop-helix protein 41; TFAP 4; TFAP4; TFAP4 protein; TFAP4_HUMAN; Transcription factor AP 4; Transcription factor AP-4; Transcription factor AP4;

Immunogens

Immunogen:

Uniprot:

Q01664(TFAP4_HUMAN) >>Visit HPA database.

Gene(ID):

TFAP4(7023)

Sequence:

Q01664 TFAP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01664 As Substrate

Q01664

Site	PTM Type	Source
Y3	Phosphorylation	Uniprot
T8	Phosphorylation	Uniprot
K10	Ubiquitination	Uniprot
S13	Phosphorylation	Uniprot
S30	Phosphorylation	Uniprot
T37	Phosphorylation	Uniprot
K103	Ubiquitination	Uniprot
K114	Ubiquitination	Uniprot
S121	Phosphorylation	Uniprot
S123	Phosphorylation	Uniprot
S124	Phosphorylation	Uniprot
K126	Ubiquitination	Uniprot
K133	Ubiquitination	Uniprot
S139	Phosphorylation	Uniprot
K147	Ubiquitination	Uniprot
K187	Ubiquitination	Uniprot
K189	Ubiquitination	Uniprot
K211	Ubiquitination	Uniprot
T282	Phosphorylation	Uniprot
K299	Ubiquitination	Uniprot
S332	Phosphorylation	Uniprot

Research Backgrounds

Q01664_TFAP4_HUMAN

Function:

Transcription factor that activates both viral and cellular genes by binding to the symmetrical DNA sequence 5'-CAGCTG-3'.

Subcellular Location:

Nucleus.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Homodimer.

Research Fields

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). circ_0006988 promotes gastric cancer cell proliferation, migration and invasion through miRNA-92a-2-5p/TFAP4 axis. Epigenomics, 2024 (PubMed: 39400106) [IF=3.0]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TFAP4 Antibody - #DF13624