PARN Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat, using PARN Antibody.

Product:	PARN Antibody
Catalog:	DF13623
Description:	Rabbit polyclonal antibody to PARN
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	73kDa; 73kD(Calculated).
Uniprot:	O95453
RRID:	AB_2846642

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Zebrafish(100%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PARN Antibody detects endogenous levels of total PARN.

RRID:

AB_2846642
Cite Format: Affinity Biosciences Cat# DF13623, RRID:AB_2846642.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DAN; Deadenylating nuclease; Deadenylation nuclease; PARN; PARN_HUMAN; Poly A specific ribonuclease; Poly(A) specific ribonuclease; Poly(A)-specific ribonuclease PARN; Polyadenylate specific ribonuclease; Polyadenylate-specific ribonuclease;

Immunogens

Immunogen:

Uniprot:

O95453(PARN_HUMAN) >>Visit HPA database.

Gene(ID):

PARN(5073)

Expression:

O95453 PARN_HUMAN:

Ubiquitous.

Sequence:

O95453 PARN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKIHVSARSKLIEPFFNKLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVKIAVNTSKYAESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTAPSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEPLSEGRKKAKKLKRMKKELSPAGSISKNSPATLFEVPDTW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Zebrafish

100

Xenopus

100

Chicken

100

Pig

Bovine

Sheep

Dog

Rabbit

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95453 As Substrate

O95453

Site	PTM Type	Enzyme	Source
K9	Ubiquitination		Uniprot
S61	Phosphorylation		Uniprot
K82	Ubiquitination		Uniprot
S151	Phosphorylation		Uniprot
Y161	Phosphorylation		Uniprot
S163	Phosphorylation		Uniprot
T166	Phosphorylation		Uniprot
S167	Phosphorylation		Uniprot
K168	Ubiquitination		Uniprot
T172	Phosphorylation		Uniprot
K179	Ubiquitination		Uniprot
K198	Ubiquitination		Uniprot
K220	Acetylation		Uniprot
K223	Ubiquitination		Uniprot
Y238	Phosphorylation		Uniprot
S242	Phosphorylation		Uniprot
K243	Acetylation		Uniprot
K250	Acetylation		Uniprot
K259	Ubiquitination		Uniprot
K326	Ubiquitination		Uniprot
S330	Phosphorylation		Uniprot
T341	Phosphorylation		Uniprot
S342	Phosphorylation		Uniprot
K348	Ubiquitination		Uniprot
S409	Phosphorylation		Uniprot
K413	Ubiquitination		Uniprot
K421	Ubiquitination		Uniprot
T451	Phosphorylation		Uniprot
K499	Acetylation		Uniprot
K499	Ubiquitination		Uniprot
S530	Phosphorylation		Uniprot
K532	Ubiquitination		Uniprot
Y552	Phosphorylation		Uniprot
S557	Phosphorylation	P49137 (MAPKAPK2)	Uniprot
S562	Phosphorylation		Uniprot
K566	Acetylation		Uniprot
K566	Ubiquitination		Uniprot
S570	Phosphorylation		Uniprot
S572	Phosphorylation		Uniprot
S583	Phosphorylation		Uniprot
S587	Phosphorylation		Uniprot
T589	Phosphorylation		Uniprot
S619	Phosphorylation		Uniprot
S623	Phosphorylation		Uniprot
S625	Phosphorylation		Uniprot
S628	Phosphorylation		Uniprot
T631	Phosphorylation		Uniprot
T638	Phosphorylation		Uniprot

Research Backgrounds

O95453_PARN_HUMAN

Function:

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. Also able to recognize and trim poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability.

PTMs:

Phosphorylation by MAPKAPK2, preventing GADD45A mRNA degradation after genotoxic stress.

Subcellular Location:

Nucleus. Cytoplasm. Nucleus>Nucleolus.
Note: Some nuclear fraction is nucleolar.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Homodimer. Found in a mRNA decay complex with RENT1, RENT2 and RENT3B. Interacts with KHSRP. Interacts with CELF1/CUGBP1. Interacts with ZC3HAV1 in an RNA-independent manner. Interacts with DHX36.

Family&Domains:

Belongs to the CAF1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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PARN Antibody - #DF13623