Repulsive Guidance Molecule A Antibody

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from Rat Brain, using Repulsive Guidance Molecule A Antibody.

Western blot analysis of extracts from HepG2 cells, using Repulsive Guidance Molecule A Antibody.

DF13622 staining Hela cells by IF/ICC.

Figure 8 A RhoA inhibitor prevents the PTBP1 knockdown induced reprogramming.

Product:	Repulsive Guidance Molecule A Antibody
Catalog:	DF13622
Description:	Rabbit polyclonal antibody to Repulsive Guidance Molecule A
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	49kDa; 49kD(Calculated).
Uniprot:	Q96B86
RRID:	AB_2846641

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

Repulsive Guidance Molecule A Antibody detects endogenous levels of total Repulsive Guidance Molecule A.

RRID:

AB_2846641
Cite Format: Affinity Biosciences Cat# DF13622, RRID:AB_2846641.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA; RGMA_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q96B86(RGMA_HUMAN) >>Visit HPA database.

Gene(ID):

RGMA(56963)

Sequence:

Q96B86 RGMA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQPPRERLVVTGRAGWMGMGRGAGRSALGFWPTLAFLLCSFPAATSPCKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYDRTRDLPGRAAAGLPLAPRPLLGALVPLLALLPVFC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Zebrafish

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96B86 As Substrate

Q96B86

Site	PTM Type	Enzyme	Source
T128	Phosphorylation		Uniprot
Y276	Phosphorylation		Uniprot

Research Backgrounds

Q96B86_RGMA_HUMAN

Function:

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

PTMs:

Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.

Subcellular Location:

Cell membrane>Lipid-anchor.

Subunit Structure:

Interacts with NEO1, BMP2 and BMP4.

Family&Domains:

Belongs to the repulsive guidance molecule (RGM) family.

References

1). PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor. Theranostics, 2022 (PubMed: 35664063) [IF=12.4]

Application: WB Species: Human Sample: U251 cells

Figure 8 A RhoA inhibitor prevents the PTBP1 knockdown induced reprogramming. (A-C) Western blot study of NTN4 and RGMA protein in U251 cells infected with sh-Luci and sh-PTBP1 for 3 and 7 days. (n = 3). GAPDH was used as an internal reference protein. (D) RhoA activity measured by G-LISA. (n = 3). (E-G) TUJ1 and KI67 positive rates of five distinct groups constituted of lentivirus (sh-Luci or sh-PTBP1) and Rhosin (vehicle, 10 µM, and 30 µM; nine random fields from triplicate samples were recorded for quantification; TUJ1+ (%) = TUJ1+ M-cherry+/M-cherry+; KI67+ (%) = KI67+ M-cherry+/M-cherry+; M-cherry+ cells = 286-538 for each condition). (H) Pathways involved in PTBP1 knockdown-induced reprogramming. The data are presented as mean ± SD. **P < 0.01, *** P < 0.001 vs. sh-Luci-3d or sh-PTBP1 + vehicle group. Dpi (d): days post infection; ND: not detected; NS: no significance. Scale: 100 µm.

2). miR-106b-5p upregulation is associated with microglial activation and inflammation in the mouse hippocampus following status epilepticus. EXPERIMENTAL BRAIN RESEARCH, 2021 (PubMed: 34476536) [IF=2.0]

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Repulsive Guidance Molecule A Antibody - #DF13622