GABA A receptor pi Antibody | Affinity Biosciences

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Western blot analysis of extracts from Jurkat, using GABA A receptor pi Antibody.

Product:	GABA A receptor pi Antibody
Catalog:	DF13621
Description:	Rabbit polyclonal antibody to GABA A receptor pi
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine
Mol.Wt.:	50kDa; 51kD(Calculated).
Uniprot:	O00591
RRID:	AB_2846640

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%)

Clonality:

Polyclonal

Specificity:

GABA A receptor pi Antibody detects endogenous levels of total GABA A receptor pi.

RRID:

AB_2846640
Cite Format: Affinity Biosciences Cat# DF13621, RRID:AB_2846640.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GABA(A) receptor subunit pi; GABRP; Gamma aminobutyric acid (GABA) A receptor pi; Gamma aminobutyric acid A receptor pi; Gamma aminobutyric acid receptor pi subunit; Gamma aminobutyric acid receptor subunit pi; Gamma-aminobutyric acid receptor subunit pi; GBRP_HUMAN; MGC126386; MGC126387;

Immunogens

Immunogen:

Uniprot:

O00591(GBRP_HUMAN) >>Visit HPA database.

Gene(ID):

GABRP(2568)

Expression:

O00591 GBRP_HUMAN:

Most abundant in the uterus, also expressed in lung, thymus and prostate.

Sequence:

O00591 GBRP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNYSLHLAFVCLSLFTERMCIQGSQFNVEVGRSDKLSLPGFENLTAGYNKFLRPNFGGEPVQIALTLDIASISSISESNMDYTATIYLRQRWMDQRLVFEGNKSFTLDARLVEFLWVPDTYIVESKKSFLHEVTVGNRLIRLFSNGTVLYALRITTTVACNMDLSKYPMDTQTCKLQLESWGYDGNDVEFTWLRGNDSVRGLEHLRLAQYTIERYFTLVTRSQQETGNYTRLVLQFELRRNVLYFILETYVPSTFLVVLSWVSFWISLDSVPARTCIGVTTVLSMTTLMIGSRTSLPNTNCFIKAIDVYLGICFSFVFGALLEYAVAHYSSLQQMAAKDRGTTKEVEEVSITNIINSSISSFKRKISFASIEISSDNVDYSDLTMKTSDKFKFVFREKMGRIVDYFTIQNPSNVDHYSKLLFPLIFMLANVFYWAYYMYF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00591 As Substrate

O00591

Site	PTM Type	Source
S78	Phosphorylation	Uniprot
Y82	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
Y244	Phosphorylation	Uniprot
S367	Phosphorylation	Uniprot
Y380	Phosphorylation	Uniprot

Research Backgrounds

O00591_GBRP_HUMAN

Function:

GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. In the uterus, the function of the receptor appears to be related to tissue contractility. The binding of this pI subunit with other GABA(A) receptor subunits alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone.

Subcellular Location:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Most abundant in the uterus, also expressed in lung, thymus and prostate.

Subunit Structure:

Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and epsilon. A sixth class of subunit: Rho form homomeric GABA receptors that do not appear to coexist with GABA(A) receptor subunits but with GABA(C) receptor subunits. Subunit Pi can also bind this complex.

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Substance dependence > Morphine addiction.

· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

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GABA A receptor pi Antibody - #DF13621