Lefty Antibody - #DF13617

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Product: Lefty Antibody
Catalog: DF13617
Description: Rabbit polyclonal antibody to Lefty
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 40kDa; 41kD(Calculated).
Uniprot: O75610
RRID: AB_2846636

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Lefty Antibody detects endogenous levels of total Lefty.
RRID:
AB_2846636
Cite Format: Affinity Biosciences Cat# DF13617, RRID:AB_2846636.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI450052; Ebaf; Left right determination factor 1; Left right determination factor; Left right determination factor B; left-right determination factor; LEFT-RIGHT DETERMINATION FACTOR 1; LEFTY1; left-right determination, factor B; LEFT-RIGHT DETERMINATION, FACTOR B; LEFTY B; LEFTB; LEFTY 1; Lefty; lefty-1; LEFTY1; LEFTY1, MOUSE, HOMOLOG OF; LEFTYB; OTTHUMP00000035572; Protein lefty-1; Protein lefty-B; RGD1561867; Stimulated by retinoic acid gene 3 protein; STRA3; TGF beta 4; TGF-beta-4; Tgfb4; Transforming growth factor beta 4; Transforming growth factor beta-4;

Immunogens

Immunogen:
Uniprot:

O75610(LFTY1_HUMAN) >>Visit HPA database.

Gene(ID):
LEFTY1(10637)
Sequence:
MQPLWLCWALWVLPLASPGAALTGEQLLGSLLRQLQLKEVPTLDRADMEELVIPTHVRAQYVALLQRSHGDRSRGKRFSQSFREVAGRFLALEASTHLLVFGMEQRLPPNSELVQAVLRLFQEPVPKAALHRHGRLSPRSARARVTVEWLRVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWQQLSRPRQPLLLQVSVQREHLGPLASGAHKLVRFASQGAPAGLGEPQLELHTLDLGDYGAQGDCDPEAPMTEGTRCCRQEMYIDLQGMKWAENWVLEPPGFLAYECVGTCRQPPEALAFKWPFLGPRQCIASETDSLPMIVSIKEGGRTRPQVVSLPNMRVQKCSCASDGALVPRRLQP

PTMs - O75610 As Substrate

Site PTM Type Enzyme
S79 Phosphorylation
S81 Phosphorylation

Research Backgrounds

Function:

Required for left-right axis determination as a regulator of LEFTY2 and NODAL.

PTMs:

The processing of the protein may also occur at the second R-X-X-R site located at AA 132-135. Processing appears to be regulated in a cell-type specific manner.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

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