XAF1 Antibody - #DF13614
| Product: | XAF1 Antibody |
| Catalog: | DF13614 |
| Description: | Rabbit polyclonal antibody to XAF1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Mol.Wt.: | 34kDa; 35kD(Calculated). |
| Uniprot: | Q6GPH4 |
| RRID: | AB_2846633 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF13614, RRID:AB_2846633.
Fold/Unfold
BIRC 4 binding protein; BIRC4 binding protein; BIRC4-binding protein; BIRC4BP; HSXIAPAF 1; HSXIAPAF1; XAF 1; XAF1; XAF1_HUMAN; XIAP associated factor 1; XIAP-associated factor 1; XIAPAF1;
Immunogens
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
- Q6GPH4 XAF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGDFSVCRNCKRHVVSANFTLHEAYCLRFLVLCPECEEPVPKETMEEHCKLEHQQVGCTMCQQSMQKSSLEFHKANECQERPVECKFCKLDMQLSKLELHESYCGSRTELCQGCGQFIMHRMLAQHRDVCRSEQAQLGKGERISAPEREIYCHYCNQMIPENKYFHHMGKCCPDSEFKKHFPVGNPEILPSSLPSQAAENQTSTMEKDVRPKTRSINRFPLHSESSSKKAPRSKNKTLDPLLMSEPKPRTSSPRGDKAAYDILRRCSQCGILLPLPILNQHQEKCRWLASSKGKQVRNFS
PTMs - Q6GPH4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S70 | Phosphorylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| Y104 | Phosphorylation | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| Y155 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| Y261 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot |
Research Backgrounds
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
Cytoplasm. Nucleus. Mitochondrion.
Note: Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment.
Nucleus.
Nucleus.
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level).
Interacts with BIRC4; the interaction is not detected in. Interacts with BIRC1, BIRC2, BIRC3, BIRC7 and BIRC8. Part of an complex consisting of BIRC4, XAF1 and BIRC5; the complex formation requires IFN-beta stimulation. Interacts with RNF114, the interaction increases XAF1 stability and proapoptotic effects, and may regulate IFN signaling.
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