MBD4 Antibody - #DF13611

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Product: MBD4 Antibody
Catalog: DF13611
Description: Rabbit polyclonal antibody to MBD4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 66kDa; 66kD(Calculated).
Uniprot: O95243
RRID: AB_2846630

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%)
Clonality:
Polyclonal
Specificity:
MBD4 Antibody detects endogenous levels of total MBD4.
RRID:
AB_2846630
Cite Format: Affinity Biosciences Cat# DF13611, RRID:AB_2846630.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3 N(4) ethenocytosine glycosylase; G/5 fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; MBD 4; MBD4; MBD4_HUMAN; MED 1; MED1; Methyl CpG binding domain protein 4; Methyl CpG binding endonuclease 1; Methyl CpG binding protein MBD4; Methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch specific DNA N glycosylase; Mismatch-specific DNA N-glycosylase; Putative methyl CpG binding protein;

Immunogens

Immunogen:
Uniprot:

O95243(MBD4_HUMAN) >>Visit HPA database.

Gene(ID):
MBD4(8930)
Sequence:
MGTTGLESLSLGDRGAAPTVTSSERLVPDPPNDLRKEDVAMELERVGEDEEQMMIKRSSECNPLLQEPIASAQFGATAGTECRKSVPCGWERVVKQRLFGKTAGRFDVYFISPQGLKFRSKSSLANYLHKNGETSLKPEDFDFTVLSKRGIKSRYKDCSMAALTSHLQNQSNNSNWNLRTRSKCKKDVFMPPSSSSELQESRGLSNFTSTHLLLKEDEGVDDVNFRKVRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTGEKIFQEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
67
Rabbit
63
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95243 As Substrate

Site PTM Type Enzyme
K36 Ubiquitination
K56 Ubiquitination
Y109 Phosphorylation
S112 Phosphorylation
K117 Ubiquitination
K137 Ubiquitination
K148 Ubiquitination
S165 Phosphorylation P17252 (PRKCA)
K215 Ubiquitination
K232 Acetylation
K234 Acetylation
K239 Acetylation
K239 Sumoylation
K239 Ubiquitination
S253 Phosphorylation
S255 Phosphorylation
S262 Phosphorylation P17252 (PRKCA)
K270 Sumoylation
K281 Ubiquitination
S304 Phosphorylation
S308 Phosphorylation
S316 Phosphorylation
S318 Phosphorylation
S321 Phosphorylation
S325 Phosphorylation
T329 Phosphorylation
S330 Phosphorylation
K335 Ubiquitination
K368 Sumoylation
K368 Ubiquitination
K377 Ubiquitination
S387 Phosphorylation
K397 Ubiquitination
S415 Phosphorylation
S428 Phosphorylation
T439 Phosphorylation
K484 Ubiquitination
K504 Ubiquitination
K518 Ubiquitination
K526 Ubiquitination
K529 Ubiquitination
K554 Ubiquitination
K562 Ubiquitination
K576 Ubiquitination

Research Backgrounds

Function:

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with MLH1.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

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