PRDM2 Antibody - #DF13607

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Product: PRDM2 Antibody
Catalog: DF13607
Description: Rabbit polyclonal antibody to PRDM2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine
Mol.Wt.: 188kDa; 189kD(Calculated).
Uniprot: Q13029
RRID: AB_2846626

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%)
Clonality:
Polyclonal
Specificity:
PRDM2 Antibody detects endogenous levels of total PRDM2.
RRID:
AB_2846626
Cite Format: Affinity Biosciences Cat# DF13607, RRID:AB_2846626.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GATA 3 binding protein G3B; GATA-3-binding protein G3B; HUMHOXY1; Lysine N methyltransferase 8; Lysine N-methyltransferase 8; MTB ZF; MTB-ZF; MTE binding protein; MTE-binding protein; PR domain containing 2 with ZNF domain; PR domain containing protein 2; PR domain zinc finger protein 2; PR domain-containing protein 2; PRDM2; PRDM2_HUMAN; Retinoblastoma protein binding zinc finger protein; Retinoblastoma protein interacting zinc- inger protein; Retinoblastoma protein-interacting zinc finger protein; RIZ; RIZ-LSB; RIZ1; RIZ2; Zinc finger DNA binding protein; Zinc finger protein RIZ;

Immunogens

Immunogen:
Uniprot:

Q13029(PRDM2_HUMAN) >>Visit HPA database.

Gene(ID):
PRDM2(7799)
Expression:
Q13029 PRDM2_HUMAN:

Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.

Sequence:
MNQNTTEPVAATETLAEVPEHVLRGLPEEVRLFPSAVDKTRIGVWATKPILKGKKFGPFVGDKKKRSQVKNNVYMWEVYYPNLGWMCIDATDPEKGNWLRYVNWACSGEEQNLFPLEINRAIYYKTLKPIAPGEELLVWYNGEDNPEIAAAIEEERASARSKRSSPKSRKGKKKSQENKNKGNKIQDIQLKTSEPDFTSANMRDSAEGPKEDEEKPSASALEQPATLQEVASQEVPPELATPAPAWEPQPEPDERLEAAACEVNDLGEEEEEEEEEDEEEEEDDDDDELEDEGEEEASMPNENSVKEPEIRCDEKPEDLLEEPKTTSEETLEDCSEVTPAMQIPRTKEEANGDVFETFMFPCQHCERKFTTKQGLERHMHIHISTVNHAFKCKYCGKAFGTQINRRRHERRHEAGLKRKPSQTLQPSEDLADGKASGENVASKDDSSPPSLGPDCLIMNSEKASQDTINSSVVEENGEVKELHPCKYCKKVFGTHTNMRRHQRRVHERHLIPKGVRRKGGLEEPQPPAEQAQATQNVYVPSTEPEEEGEADDVYIMDISSNISENLNYYIDGKIQTNNNTSNCDVIEMESASADLYGINCLLTPVTVEITQNIKTTQVPVTEDLPKEPLGSTNSEAKKRRTASPPALPKIKAETDSDPMVPSCSLSLPLSISTTEAVSFHKEKSVYLSSKLKQLLQTQDKLTPAGISATEIAKLGPVCVSAPASMLPVTSSRFKRRTSSPPSSPQHSPALRDFGKPSDGKAAWTDAGLTSKKSKLESHSDSPAWSLSGRDERETVSPPCFDEYKMSKEWTASSAFSSVCNQQPLDLSSGVKQKAEGTGKTPVQWESVLDLSVHKKHCSDSEGKEFKESHSVQPTCSAVKKRKPTTCMLQKVLLNEYNGIDLPVENPADGTRSPSPCKSLEAQPDPDLGPGSGFPAPTVESTPDVCPSSPALQTPSLSSGQLPPLLIPTDPSSPPPCPPVLTVATPPPPLLPTVPLPAPSSSASPHPCPSPLSNATAQSPLPILSPTVSPSPSPIPPVEPLMSAASPGPPTLSSSSSSSSSSSSFSSSSSSSSPSPPPLSAISSVVSSGDNLEASLPMISFKQEELENEGLKPREEPQSAAEQDVVVQETFNKNFVCNVCESPFLSIKDLTKHLSIHAEEWPFKCEFCVQLFKDKTDLSEHRFLLHGVGNIFVCSVCKKEFAFLCNLQQHQRDLHPDKVCTHHEFESGTLRPQNFTDPSKAHVEHMQSLPEDPLETSKEEEELNDSSEELYTTIKIMASGIKTKDPDVRLGLNQHYPSFKPPPFQYHHRNPMGIGVTATNFTTHNIPQTFTTAIRCTKCGKGVDNMPELHKHILACASASDKKRYTPKKNPVPLKQTVQPKNGVVVLDNSGKNAFRRMGQPKRLNFSVELSKMSSNKLKLNALKKKNQLVQKAILQKNKSAKQKADLKNACESSSHICPYCNREFTYIGSLNKHAAFSCPKKPLSPPKKKVSHSSKKGGHSSPASSDKNSNSNHRRRTADAEIKMQSMQTPLGKTRARSSGPTQVPLPSSSFRSKQNVKFAASVKSKKPSSSSLRNSSPIRMAKITHVEGKKPKAVAKNHSAQLSSKTSRSLHVRVQKSKAVLQSKSTLASKKRTDRFNIKSRERSGGPVTRSLQLAAAADLSENKREDGSAKQELKDFSYSLRLASRCSPPAAPYITRQYRKVKAPAAAQFQGPFFKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Xenopus
75
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13029 As Substrate

Site PTM Type Enzyme
T47 Phosphorylation
K63 Ubiquitination
T198 Phosphorylation
S199 Phosphorylation
S421 Phosphorylation
T423 Phosphorylation
S427 Phosphorylation
S447 Phosphorylation
S471 Phosphorylation
R508 Methylation
R516 Methylation
K626 Ubiquitination
T641 Phosphorylation
S643 Phosphorylation
K700 Ubiquitination
T702 Phosphorylation
S720 Phosphorylation
T737 Phosphorylation
S738 Phosphorylation
S739 Phosphorylation
S742 Phosphorylation
S743 Phosphorylation
S747 Phosphorylation
K774 Ubiquitination
S781 Phosphorylation
S785 Phosphorylation
S796 Phosphorylation
S846 Phosphorylation
S876 Phosphorylation
T885 Phosphorylation
Y896 Phosphorylation
T910 Phosphorylation
S912 Phosphorylation
S914 Phosphorylation
S1054 Phosphorylation
S1055 Phosphorylation
S1056 Phosphorylation
S1057 Phosphorylation
S1118 Phosphorylation
S1141 Phosphorylation
T1255 Phosphorylation
S1256 Phosphorylation
S1265 Phosphorylation
S1266 Phosphorylation
Y1295 Phosphorylation
S1297 Phosphorylation
S1359 Phosphorylation
S1414 Phosphorylation
K1425 Acetylation
K1431 Acetylation
S1484 Phosphorylation
S1491 Phosphorylation
S1509 Phosphorylation
S1511 Phosphorylation
T1529 Phosphorylation
S1571 Phosphorylation
T1585 Phosphorylation
K1597 Ubiquitination
K1606 Ubiquitination
S1641 Phosphorylation
S1645 Phosphorylation
S1652 Phosphorylation

Research Backgrounds

Function:

S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in retinoblastoma cell lines and in brain tumors. Also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3.

Subunit Structure:

Binds to the retinoblastoma protein (RB). Interacts with GATA3.

Family&Domains:

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Research Fields

· Metabolism > Amino acid metabolism > Lysine degradation.

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