Surfactant protein D Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/4

Western blot analysis of extracts from 3T3, using Surfactant protein D Antibody.

Western blot analysis of extracts from Rat lung, using Surfactant protein D Antibody.

DF13601 staining HepG2 cells by IF/ICC.

Fig.

Product:	Surfactant protein D Antibody
Catalog:	DF13601
Description:	Rabbit polyclonal antibody to Surfactant protein D
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	38kDa; 38kD(Calculated).
Uniprot:	P35247
RRID:	AB_2846620

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Surfactant protein D Antibody detects endogenous levels of total Surfactant protein D.

RRID:

AB_2846620
Cite Format: Affinity Biosciences Cat# DF13601, RRID:AB_2846620.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COLEC 7; COLEC7; Collectin-7; Collectin7; Lung surfactant protein D; PSP D; PSP-D; PSP-D Surfactant protein D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; Pulmonary surfactant associated protein PSP-D; Pulmonary surfactant-associated protein D; SFTP 4; SFTP4; SFTPD; SFTPD_HUMAN; SP D; SP-D; Surfactant associated protein pulmonary 4; Surfactant protein D; Surfactant pulmonary associated protein D;

Immunogens

Immunogen:

Uniprot:

P35247(SFTPD_HUMAN) >>Visit HPA database.

Gene(ID):

SFTPD(6441)

Expression:

P35247 SFTPD_HUMAN:

Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

Sequence:

P35247 SFTPD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLFLLSALVLLTQPLGYLEAEMKTYSHRTMPSACTLVMCSSVESGLPGRDGRDGREGPRGEKGDPGLPGAAGQAGMPGQAGPVGPKGDNGSVGEPGPKGDTGPSGPPGPPGVPGPAGREGPLGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSAGARGLAGPKGERGVPGERGVPGNTGAAGSAGAMGPQGSPGARGPPGLKGDKGIPGDKGAKGESGLPDVASLRQQVEALQGQVQHLQAAFSQYKKVELFPNGQSVGEKIFKTAGFVKPFTEAQLLCTQAGGQLASPRSAAENAALQQLVVAKNEAAFLSMTDSKTEGKFTYPTGESLVYSNWAPGEPNDDGGSEDCVEIFTNGKWNDRACGEKRLVVCEF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35247 As Substrate

P35247

Site	PTM Type	Source
T30	Phosphorylation	Uniprot
S33	Phosphorylation	Uniprot
C35	S-Nitrosylation	Uniprot
C40	S-Nitrosylation	Uniprot

Research Backgrounds

P35247_SFTPD_HUMAN

Function:

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

PTMs:

The N-terminus is blocked.

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix. Secreted>Extracellular space>Surface film.

Tissue Specificity:

Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

Subunit Structure:

Oligomeric complex of 4 set of homotrimers.

Family&Domains:

Belongs to the SFTPD family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

References

1). GTS-21 attenuates ACE/ACE2 ratio and glycocalyx shedding in lipopolysaccharide-induced acute lung injury by targeting macrophage polarization derived ADAM-17. International immunopharmacology, 2024 (PubMed: 38310766) [IF=5.6]

Application: WB Species: Mouse Sample: lung tissue

Fig. 2. GTS-21 regulated the ACE/ACE2 ratio and glycocalyx shedding in LPS-ALI mice A-D ELISA kits were used to quantify the concentrations of major renin-angiotensin system (RAS) regulating enzymes (ACE, Ang II, ACE2, and Ang 1-7) in the lung tissue (n = 6 per group). E Protein levels of important RAS-regulating enzymes (ACE, AT1R, ACE2, and MasR) (n = 3 per group). The relative protein levels are displayed. F Levels of NF-κB phosphoproteins (p-P65 and p-IκB) (n = 3 per group). The relative protein levels are displayed. The immunofluorescence of HS, SDC-1, and surfactant protein D in the lungs is shown in G in representative images and densitometry (scar bar, 20 mm, n = 3 per group). H Evans Blue staining tested lung permeability (n = 3 for each group). Using ELISA, we assessed the BALF concentrations of HS I and HPA J; SP-D in serum K and BALF L (n = 5 for each group). M Relative protein levels of SDC-1, HPA, EXT-1, and SP-D in The lung tissues (n = 3 for each group). All data were shown as mean ± SD.

2). Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats. JOURNAL OF ETHNOPHARMACOLOGY, 2023 (PubMed: 36535334) [IF=5.4]

3). PC (16:0/14:0) ameliorates hyperoxia-induced bronchopulmonary dysplasia by upregulating claudin-1 and promoting alveolar type II cell repair. The international journal of biochemistry & cell biology, 2024 (PubMed: 38740281) [IF=4.0]

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Surfactant protein D Antibody - #DF13601