Hemoglobin subunit beta Antibody - #DF13595

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Product: Hemoglobin subunit beta Antibody
Catalog: DF13595
Description: Rabbit polyclonal antibody to Hemoglobin subunit beta
Application: WB IHC
Reactivity: Human, Mouse, Monkey
Prediction: Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 15kDa; 16kD(Calculated).
Uniprot: P68871
RRID: AB_2846614

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Monkey
Prediction:
Pig(100%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
Hemoglobin subunit beta Antibody detects endogenous levels of total Hemoglobin subunit beta.
RRID:
AB_2846614
Cite Format: Affinity Biosciences Cat# DF13595, RRID:AB_2846614.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ba1; Beta 1 globin; beta globin; beta globin chain; BETA GLOBIN TYPE; BETA THALASSEMIAS; Beta-A1-globin; CD113t C; CD113t-C; ERYTHREMIA; HBB; Hemoglobin beta 1 chain; hemoglobin beta chain; hemoglobin beta chain complex; Hemoglobin beta chain, major form; HEMOGLOBIN BETA LOCUS; Hemoglobin beta-1 chain; Hemoglobin subunit beta-1; METHEMOGLOBINEMIA;

Immunogens

Immunogen:
Uniprot:

P68871(HBB_HUMAN) >>Visit HPA database.

Gene(ID):
HBB(3043)
Expression:
P68871 HBB_HUMAN:

Red blood cells.

Sequence:
MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
100
Chicken
88
Horse
86
Bovine
86
Dog
75
Sheep
71
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P68871 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
K9 Acetylation
S10 Phosphorylation
T13 Phosphorylation
K18 Acetylation
Y36 Phosphorylation
T39 Phosphorylation
S45 Phosphorylation
S50 O-Glycosylation
S50 Phosphorylation
T51 Phosphorylation
K60 Acetylation
K62 Acetylation
K66 Acetylation
S73 O-Glycosylation
S73 Phosphorylation
K83 Acetylation
T85 O-Glycosylation
T88 Phosphorylation
S90 Phosphorylation
C94 S-Nitrosylation
C113 S-Nitrosylation
K121 Acetylation
T124 Phosphorylation
Y131 Phosphorylation
K133 Acetylation
K145 Acetylation
Y146 Phosphorylation

Research Backgrounds

Function:

Involved in oxygen transport from the lung to the various peripheral tissues.

LVV-hemorphin-7 potentiates the activity of bradykinin, causing a decrease in blood pressure.

functions as an endogenous inhibitor of enkephalin-degrading enzymes such as DPP3, and as a selective antagonist of the P2RX3 receptor which is involved in pain signaling, these properties implicate it as a regulator of pain and inflammation.

PTMs:

Glucose reacts non-enzymatically with the N-terminus of the beta chain to form a stable ketoamine linkage. This takes place slowly and continuously throughout the 120-day life span of the red blood cell. The rate of glycation is increased in patients with diabetes mellitus.

S-nitrosylated; a nitric oxide group is first bound to Fe(2+) and then transferred to Cys-94 to allow capture of O(2).

Acetylated on Lys-60, Lys-83 and Lys-145 upon aspirin exposure.

Tissue Specificity:

Red blood cells.

Subunit Structure:

Heterotetramer of two alpha chains and two beta chains in adult hemoglobin A (HbA). Heterotetramer of two zeta chains and two beta chains in hemoglobin Portland-2, detected in fetuses and neonates with homozygous alpha-thalassemia.

Family&Domains:

Belongs to the globin family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

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