GPAT-2 Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from Human pancreas, using GPAT-2 Antibody.

Western blot analysis of extracts from Rat liver, using GPAT-2 Antibody.

Product:	GPAT-2 Antibody
Catalog:	DF13583
Description:	Rabbit polyclonal antibody to GPAT-2
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.:	87kDa; 88kD(Calculated).
Uniprot:	Q6NUI2
RRID:	AB_2846602

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

GPAT-2 Antibody detects endogenous levels of total GPAT-2.

RRID:

AB_2846602
Cite Format: Affinity Biosciences Cat# DF13583, RRID:AB_2846602.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glycerol-3-phosphate acyltransferase 2; GPAT-2; GPAT2; GPAT2_HUMAN; mitochondrial; xGPAT1;

Immunogens

Immunogen:

Uniprot:

Q6NUI2(GPAT2_HUMAN) >>Visit HPA database.

Gene(ID):

GPAT2(150763)

Sequence:

Q6NUI2 GPAT2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATMLEGRCQTQPRSSPSGREASLWSSGFGMKLEAVTPFLGKYRPFVGRCCQTCTPKSWESLFHRSITDLGFCNVILVKEENTRFRGWLVRRLCYFLWSLEQHIPPCQDVPQKIMESTGVQNLLSGRVPGGTGEGQVPDLVKKEVQRILGHIQAPPRPFLVRLFSWALLRFLNCLFLNVQLHKGQMKMVQKAAQAGLPLVLLSTHKTLLDGILLPFMLLSQGLGVLRVAWDSRACSPALRALLRKLGGLFLPPEASLSLDSSEGLLARAVVQAVIEQLLVSGQPLLIFLEEPPGALGPRLSALGQAWVGFVVQAVQVGIVPDALLVPVAVTYDLVPDAPCDIDHASAPLGLWTGALAVLRSLWSRWGCSHRICSRVHLAQPFSLQEYIVSARSCWGGRQTLEQLLQPIVLGQCTAVPDTEKEQEWTPITGPLLALKEEDQLLVRRLSCHVLSASVGSSAVMSTAIMATLLLFKHQKLLGEFSWLTEEILLRGFDVGFSGQLRSLLQHSLSLLRAHVALLRIRQGDLLVVPQPGPGLTHLAQLSAELLPVFLSEAVGACAVRGLLAGRVPPQGPWELQGILLLSQNELYRQILLLMHLLPQDLLLLKPCQSSYCYCQEVLDRLIQCGLLVAEETPGSRPACDTGRQRLSRKLLWKPSGDFTDSDSDDFGEADGRYFRLSQQSHCPDFFLFLCRLLSPLLKAFAQAAAFLRQGQLPDTELGYTEQLFQFLQATAQEEGIFECADPKLAISAVWTFRDLGVLQQTPSPAGPRLHLSPTFASLDNQEKLEQFIRQFICS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Rabbit

100

Pig

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6NUI2 As Substrate

Q6NUI2

Site	PTM Type	Source
K142	Ubiquitination	Uniprot
K143	Ubiquitination	Uniprot
K191	Ubiquitination	Uniprot
S203	Phosphorylation	Uniprot
T204	Phosphorylation	Uniprot
K245	Ubiquitination	Uniprot
K436	Ubiquitination	Uniprot
K650	Ubiquitination	Uniprot
K654	Ubiquitination	Uniprot
S695	Phosphorylation	Uniprot
K784	Ubiquitination	Uniprot

Research Backgrounds

Q6NUI2_GPAT2_HUMAN

Function:

Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. Required for primary processing step during piRNA biosynthesis. Molecular mechanisms by which it promotes piRNA biosynthesis are unclear and do not involve its acyltransferase activity.

Subcellular Location:

Mitochondrion outer membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with PIWIL2.

Family&Domains:

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.

Belongs to the GPAT/DAPAT family.

Research Fields

· Metabolism > Lipid metabolism > Glycerolipid metabolism.

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

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For Research Use Only.
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GPAT-2 Antibody - #DF13583