NMNAT2 Antibody - #DF13581

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Product: NMNAT2 Antibody
Catalog: DF13581
Description: Rabbit polyclonal antibody to NMNAT2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: Q9BZQ4
RRID: AB_2846600

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
NMNAT2 Antibody detects endogenous levels of total NMNAT2.
RRID:
AB_2846600
Cite Format: Affinity Biosciences Cat# DF13581, RRID:AB_2846600.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C1orf15; KIAA0479; MGC2756; NaMN adenylyltransferase 2; Nicotinamide mononucleotide adenylyltransferase 2; Nicotinamide nucleotide adenylyltransferase 2; Nicotinate-nucleotide adenylyltransferase 2; NMN adenylyltransferase 2; NMNA2_HUMAN; NMNAT 2; Nmnat2; PNAT 2; PNAT2; Pyridine nucleotide adenylyltransferase 2;

Immunogens

Immunogen:
Uniprot:

Q9BZQ4(NMNA2_HUMAN) >>Visit HPA database.

Gene(ID):
NMNAT2(23057)
Expression:
Q9BZQ4 NMNA2_HUMAN:

Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.

Sequence:
MTETTKTHVILLACGSFNPITKGHIQMFERARDYLHKTGRFIVIGGIVSPVHDSYGKQGLVSSRHRLIMCQLAVQNSDWIRVDPWECYQDTWQTTCSVLEHHRDLMKRVTGCILSNVNTPSMTPVIGQPQNETPQPIYQNSNVATKPTAAKILGKVGESLSRICCVRPPVERFTFVDENANLGTVMRYEEIELRILLLCGSDLLESFCIPGLWNEADMEVIVGDFGIVVVPRDAADTDRIMNHSSILRKYKNNIMVVKDDINHPMSVVSSTKSRLALQHGDGHVVDYLSQPVIDYILKSQLYINASG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BZQ4 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation
T21 Phosphorylation
K155 Ubiquitination
Y250 Phosphorylation
K272 Ubiquitination
Y287 Phosphorylation
Y295 Phosphorylation

Research Backgrounds

Function:

Nicotinamide/nicotinate-nucleotide adenylyltransferase that acts as an axon maintenance factor (By similarity). Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate but with a lower efficiency. Cannot use triazofurin monophosphate (TrMP) as substrate. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity prefers NAD(+), NADH and NaAD as substrates and degrades nicotinic acid adenine dinucleotide phosphate (NHD) less effectively. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NaADP(+). Axon survival factor required for the maintenance of healthy axons: acts by delaying Wallerian axon degeneration, an evolutionarily conserved process that drives the loss of damaged axons (By similarity).

PTMs:

Degraded in response to injured neurite. Degradation is probably caused by ubiquitination by MYCBP2 (By similarity). Ubiquitinated on threonine and/or serine residues by MYCBP2; consequences of threonine and/or serine ubiquitination are however unclear.

Palmitoylated; palmitoylation is required for membrane association.

Subcellular Location:

Golgi apparatus membrane>Lipid-anchor. Cytoplasmic vesicle membrane>Lipid-anchor. Cytoplasm. Cell projection>Axon.
Note: Delivered to axons with Golgi-derived cytoplasmic vesicles.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain, in particular in cerebrum, cerebellum, occipital lobe, frontal lobe, temporal lobe and putamen. Also found in the heart, skeletal muscle, pancreas and islets of Langerhans.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the eukaryotic NMN adenylyltransferase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast. Redox Biology, 2022 (PubMed: 35926314) [IF=11.4]

Application: WB    Species: Mouse    Sample:

Fig. 4. NAD + concentration and bio-synthesis changed in different subcellular compartments after DEHP treatment. Pregnant mice were administrated by DEHP (0, 5, 50 and 200 mg/kg/d) during GD 0–15. NAD+ content was measured in placental (A–D) nucleus, (E–H) cytoplasm, and (I–L) mitochondria, respectively. (M) NMNAT1, NMNAT2, and NMNAT3 representative images of Western blot. Quantifications of (N) NMNAT1, (O) NMNAT2, and (P) NMNAT3 expression in mice placenta based on band intensity were shown on the right side of the blots. All data are expressed as mean ± standard errors. (N = 6). * indicate a statistically significant difference (P < 0.05).
2). Colonic mechanism of serum NAD+ depletion induced by DEHP during pregnancy. Science of The Total Environment, 2023 (PubMed: 36781136) [IF=9.8]

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