KCC2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562, using KCC2 Antibody.

Product:	KCC2 Antibody
Catalog:	DF13580
Description:	Rabbit polyclonal antibody to KCC2
Application:	WB
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	126kDa; 126kD(Calculated).
Uniprot:	Q9H2X9
RRID:	AB_2846599

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

KCC2 Antibody detects endogenous levels of total KCC2.

RRID:

AB_2846599
Cite Format: Affinity Biosciences Cat# DF13580, RRID:AB_2846599.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Electroneutral potassium chloride cotransporter 2; Electroneutral potassium-chloride cotransporter 2; Erythroid K Cl cotransporter 2; Furosemide sensitive K Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2; KIAA1176; Neuronal K Cl cotransporter; Neuronal K-Cl cotransporter; Potassium Chloride Cotransporter; Potassium chloride transporter 5; rKCC2; S12A5; S12A5_HUMAN; SLC12A5; Solute carrier family 12 (potassium chloride transporter) member 5; Solute carrier family 12 member 5;

Immunogens

Immunogen:

Uniprot:

Q9H2X9(S12A5_HUMAN) >>Visit HPA database.

Gene(ID):

SLC12A5(57468)

Expression:

Q9H2X9 S12A5_HUMAN:

Brain specific. Detected in neuronal cells.

Sequence:

Q9H2X9 S12A5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRRFTVTSLPPAGPARSPDPESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQDQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS

PTMs - Q9H2X9 As Substrate

Q9H2X9

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T6	Phosphorylation	Q9UEW8 (STK39)	Uniprot
S27	Phosphorylation		Uniprot
S54	Phosphorylation		Uniprot
T57	Phosphorylation		Uniprot
T77	Phosphorylation		Uniprot
S78	Phosphorylation		Uniprot
S82	Phosphorylation		Uniprot
T92	Phosphorylation		Uniprot
T501	Phosphorylation		Uniprot
Y617	Phosphorylation		Uniprot
S622	Phosphorylation		Uniprot
T821	Phosphorylation		Uniprot
T831	Phosphorylation		Uniprot
T929	Phosphorylation		Uniprot
S963	Phosphorylation		Uniprot
T1030	Phosphorylation		Uniprot
T1032	Phosphorylation	Q9UEW8 (STK39)	Uniprot
Y1110	Phosphorylation		Uniprot

Research Backgrounds

Q9H2X9_S12A5_HUMAN

Function:

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (By similarity). Involved in the regulation of dendritic spine formation and maturation.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Brain specific. Detected in neuronal cells.

Subunit Structure:

Homomultimer and heteromultimer with other K-Cl cotransporters. Interacts with AP2A1 (By similarity).

Family&Domains:

Belongs to the SLC12A transporter family.

Research Fields

· Organismal Systems > Nervous system > GABAergic synapse.

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KCC2 Antibody - #DF13580