BASP1 Antibody - #DF13578

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Product: BASP1 Antibody
Catalog: DF13578
Description: Rabbit polyclonal antibody to BASP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish
Mol.Wt.: 22kDa; 23kD(Calculated).
Uniprot: P80723
RRID: AB_2846597

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%)
Clonality:
Polyclonal
Specificity:
BASP1 Antibody detects endogenous levels of total BASP1.
RRID:
AB_2846597
Cite Format: Affinity Biosciences Cat# DF13578, RRID:AB_2846597.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

22 kDa neuronal tissue enriched acidic protein; BASP 1; BASP1 protein; Brain abundant membrane attached signal protein 1; Brain Abundant Signal Protein Membrane Attached 1; Brain Acid Soluble Protein 1; CAP 23; CAP23; MGC8555; NAP 22; NAP22; Neuronal axonal membrane protein NAP 22; Neuronal tissue enriched acidic protein;

Immunogens

Immunogen:
Uniprot:

P80723(BASP1_HUMAN) >>Visit HPA database.

Gene(ID):
BASP1(10409)
Expression:
P80723 BASP1_HUMAN:

Brain.

Sequence:
MGGKLSKKKKGYNVNDEKAKEKDKKAEGAATEEEGTPKESEPQAAAEPAEAKEGKEKPDQDAEGKAEEKEGEKDAAAAKEEAPKAEPEKTEGAAEAKAEPPKAPEQEQAAPGPAAGGEAPKAAEAAAAPAESAAPAAGEEPSKEEGEPKKTEAPAAPAAQETKSDGAPASDSKPGSSEAAPSSKETPAATEAPSSTPKAQGPAASAEEPKPVEAPAANSDQTVTVKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Pig
67
Horse
67
Bovine
67
Xenopus
67
Chicken
67
Sheep
0
Dog
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P80723 As Substrate

Site PTM Type Enzyme
G2 Myristoylation
K10 Ubiquitination
Y12 Phosphorylation
K18 Ubiquitination
K25 Ubiquitination
T31 Phosphorylation
T36 Phosphorylation
K38 Ubiquitination
S40 Phosphorylation
K52 Ubiquitination
K55 Ubiquitination
K57 Ubiquitination
K65 Ubiquitination
K73 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K89 Ubiquitination
K97 Acetylation
K97 Ubiquitination
K102 Ubiquitination
K121 Ubiquitination
S132 Phosphorylation
S142 Phosphorylation
K143 Sumoylation
K143 Ubiquitination
K150 Ubiquitination
T162 Phosphorylation
K163 Sumoylation
K163 Ubiquitination
S164 Phosphorylation
S170 Phosphorylation
S172 Phosphorylation
K173 Ubiquitination
S176 Phosphorylation
S177 Phosphorylation
S182 Phosphorylation
S183 Phosphorylation
K184 Ubiquitination
T186 Phosphorylation
T190 Phosphorylation
S194 Phosphorylation
S195 Phosphorylation
T196 Phosphorylation
K198 Ubiquitination
S205 Phosphorylation
K210 Acetylation
K210 Ubiquitination
S219 Phosphorylation
K226 Ubiquitination

Research Backgrounds

Subcellular Location:

Cell membrane>Lipid-anchor. Cell projection>Growth cone.
Note: Associated with the membranes of growth cones that form the tips of elongating axons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain.

Family&Domains:

Belongs to the BASP1 family.

References

1). High BASP1 Expression is Associated with Poor Prognosis and Promotes Tumor Progression in Human Lung Adenocarcinoma. CANCER INVESTIGATION, 2021 (PubMed: 33813994) [IF=2.4]

Application: WB    Species: Human    Sample: lung adenocarcinoma cell

Figure 6. BASP1 expression in lung adenocarcinoma cell lines. (A) BASP1 protein expression level was assessed in lung adenocarcinoma cell lines using Western blot. (B) Quantitative visualization using ImageJ analysis. (C) RT-qPCR detected mRNA level of BASP1 in LUAD cell lines. (D, E) The suppression of BASP1 in the A549 cell was confirmed at both the protein and (F) mRNA levels. (G, H) The overexpression of BASP1 in H1650 was confirmed by western blot, and (I) RT-qPCR.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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