DHCR7 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from HepG2 cells, using DHCR7 Antibody.

FIG 1 (A) Cholesterol synthesis pathway.

Product:	DHCR7 Antibody
Catalog:	DF13577
Description:	Rabbit polyclonal antibody to DHCR7
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	54kDa; 54kD(Calculated).
Uniprot:	Q9UBM7
RRID:	AB_2846596

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

DHCR7 Antibody detects endogenous levels of total DHCR7.

RRID:

AB_2846596
Cite Format: Affinity Biosciences Cat# DF13577, RRID:AB_2846596.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; SLOS; Sterol delta 7 reductase; Sterol Delta;

Immunogens

Immunogen:

Uniprot:

Q9UBM7(DHCR7_HUMAN) >>Visit HPA database.

Gene(ID):

DHCR7(1717)

Expression:

Q9UBM7 DHCR7_HUMAN:

Widely expressed. Most abundant in adrenal gland, liver, testis, and brain.

Sequence:

Q9UBM7 DHCR7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBM7 As Substrate

Q9UBM7

Site	PTM Type	Source
K4	Ubiquitination	Uniprot
S5	Phosphorylation	Uniprot
K13	Ubiquitination	Uniprot
S14	Phosphorylation	Uniprot
T19	Phosphorylation	Uniprot
S25	Phosphorylation	Uniprot
K88	Ubiquitination	Uniprot
T135	Phosphorylation	Uniprot
S205	Phosphorylation	Uniprot
K231	Ubiquitination	Uniprot
K358	Ubiquitination	Uniprot
K376	Ubiquitination	Uniprot
C380	S-Nitrosylation	Uniprot
Y382	Phosphorylation	Uniprot
S397	Phosphorylation	Uniprot
K454	Ubiquitination	Uniprot

Research Backgrounds

Q9UBM7_DHCR7_HUMAN

Function:

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Tissue Specificity:

Widely expressed. Most abundant in adrenal gland, liver, testis, and brain.

Subunit Structure:

Interacts with DHCR24; this interaction regulates DHCR7 activity.

Family&Domains:

Belongs to the ERG4/ERG24 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Augmentation of 3β-hydroxysteroid-Δ24 Reductase (DHCR24) Expression Induced by Bovine Viral Diarrhea Virus Infection Facilitates Viral Replication via Promoting Cholesterol Synthesis. Journal of Virology, 2022 (PubMed: 36468862) [IF=5.4]

Application: WB Species: bovine Sample: bovine cells

FIG 1 (A) Cholesterol synthesis pathway. (B) Heat map of the key genes (transcriptomic data) and corresponding proteins (proteomic data) involved in lipid metabolism in bovine viral diarrhea virus (BVDV)-infected bovine cells. (C, D) Identification of differentially expressed genes by quantitative reverse transcription-PCR (qRT-PCR) (C) and corresponding proteins by Western blotting (D). HMGCS1, 3- hydroxy-3-methylglutaryl-coenzyme A synthase 1; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; MVK, mevalonate kinase; PMVK, phosphomevalonate kinase; MVD, diphosphomevalonate decarboxylase; FDPS, farnesyl diphosphate synthase; FDFT1, farnesyl diphosphate farnesyltransferase 1; SQLE, squalene epoxidase; GGPS, geranylgeranyl pyrophosphate synthase; LSS, lanosterol synthase; D8D7I, 3β-hydroxysterol-Δ8,7-isomerase; C5SD, 3β-hydroxysterol-C5 desaturase; DHCR7, 7-dehydrocholesterol reductase; DHCR24, 3β-hydroxysteroid-Δ24 reductase; SCD1, stearoyl-CoA desaturase; FASN, fatty acid synthase; ACACA, acetyl coenzyme A carboxylase alpha.

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DHCR7 Antibody - #DF13577