Filamin B Antibody - #DF13572

(2)  
Product: Filamin B Antibody
Catalog: DF13572
Description: Rabbit polyclonal antibody to Filamin B
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 278kDa; 278kD(Calculated).
Uniprot: O75369
RRID: AB_2846591

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Filamin B Antibody detects endogenous levels of total Filamin B.
RRID:
AB_2846591
Cite Format: Affinity Biosciences Cat# DF13572, RRID:AB_2846591.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABP 278; ABP 280 homolog; ABP-278; ABP-280 homolog; ABP278; Actin binding like protein; Actin binding protein 278; Actin-binding-like protein; AOI; Beta filamin; Beta-filamin; DKFZp686A1668; DKFZp686O033; Fh1; Filamin 1 actin binding protein 280 like; Filamin 3; Filamin B; Filamin B beta actin binding protein 278; Filamin homolog 1; Filamin-3; Filamin-B; FLN B; FLN-B; FLN1L; FLN3; FLNB; FLNB_HUMAN; LRS1; SCT; TABP; TAP; Thyroid autoantigen; Truncated ABP; Truncated actin binding protein; Truncated actin-binding protein;

Immunogens

Immunogen:
Uniprot:

O75369(FLNB_HUMAN) >>Visit HPA database.

Gene(ID):
FLNB(2317)
Expression:
O75369 FLNB_HUMAN:

Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Sequence:
MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMYRKYHQRPTFRQMQLENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQTPKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPQKPVDNAREAMQQADDWLGVPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVDTISAGQGDVMVFVEDPEGNKEEAQVTPDSDKNKTYSVEYLPKVTGLHKVTVLFAGQHISKSPFEVSVDKAQGDASKVTAKGPGLEAVGNIANKPTYFDIYTAGAGVGDIGVEVEDPQGKNTVELLVEDKGNQVYRCVYKPMQPGPHVVKIFFAGDTIPKSPFVVQVGEACNPNACRASGRGLQPKGVRIRETTDFKVDTKAAGSGELGVTMKGPKGLEELVKQKDFLDGVYAFEYYPSTPGRYSIAITWGGHHIPKSPFEVQVGPEAGMQKVRAWGPGLHGGIVGRSADFVVESIGSEVGSLGFAIEGPSQAKIEYNDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDSPYMAFIHPATGGYNPDLVRAYGPGLEKSGCIVNNLAEFTVDPKDAGKAPLKIFAQDGEGQRIDIQMKNRMDGTYACSYTPVKAIKHTIAVVWGGVNIPHSPYRVNIGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCTEAGEGDVSVGIKCDARVLSEDEEDVDFDIIHNANDTFTVKYVPPAAGRYTIKVLFASQEIPASPFRVKVDPSHDASKVKAEGPGLSKAGVENGKPTHFTVYTKGAGKAPLNVQFNSPLPGDAVKDLDIIDNYDYSHTVKYTPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSKIKLNGLENRVEVGKDQEFTVDTRGAGGQGKLDVTILSPSRKVVPCLVTPVTGRENSTAKFIPREEGLYAVDVTYDGHPVPGSPYTVEASLPPDPSKVKAHGPGLEGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPGSPFKADIEMPFDPSKVVASGPGLEHGKVGEAGLLSVDCSEAGPGALGLEAVSDSGTKAEVSIQNNKDGTYAVTYVPLTAGMYTLTMKYGGELVPHFPARVKVEPAVDTSRIKVFGPGIEGKDVFREATTDFTVDSRPLTQVGGDHIKAHIANPSGASTECFVTDNADGTYQVEYTPFEKGLHVVEVTYDDVPIPNSPFKVAVTEGCQPSRVQAQGPGLKEAFTNKPNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYIPFAPGDYDVNITYGGAHIPGSPFRVPVKDVVDPSKVKIAGPGLGSGVRARVLQSFTVDSSKAGLAPLEVRVLGPRGLVEPVNVVDNGDGTHTVTYTPSQEGPYMVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSSYGVPASLPVDFAIDARDAGEGLLAVQITDQEGKPKRAIVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDDIPLSPYRIRATQTGDASKCLATGPGIASTVKTGEEVGFVVDAKTAGKGKVTCTVLTPDGTEAEADVIENEDGTYDIFYTAAKPGTYVIYVRFGGVDIPNSPFTVMATDGEVTAVEEAPVNACPPGFRPWVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEITGEVHMPSGKTATPEIVDNKDGTVTVRYAPTEVGLHEMHIKYMGSHIPESPLQFYVNYPNSGSVSAYGPGLVYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPGSPFTAKITDDSRRCSQVKLGSAADFLLDISETDLSSLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREVGEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGRGLSEGRTFEMSDFIVDTRDAGYGGISLAVEGPSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVKESITRTSRAPSVATVGSICDLNLKIPEINSSDMSAHVTSPSGRVTEAEIVPMGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPFQFTVGPLGEGGAHKVRAGGPGLERGEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVSYIAQEPGNYEVSIKFNDEHIPESPYLVPVIAPSDDARRLTVMSLQESGLKVNQPASFAIRLNGAKGKIDAKVHSPSGAVEECHVSELEPDKYAVRFIPHENGVHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYGTGLEGGTTGIQSEFFINTTRAGPGTLSVTIEGPSKVKMDCQETPEGYKVMYTPMAPGNYLISVKYGGPNHIVGSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKASSDASKVTSKGAGLSKAFVGQKSSFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75369 As Substrate

Site PTM Type Enzyme
K6 Ubiquitination
K15 Acetylation
K15 Methylation
K15 Ubiquitination
K16 Ubiquitination
T21 Phosphorylation
T23 Phosphorylation
K31 Ubiquitination
R36 Methylation
S58 Phosphorylation
K60 Ubiquitination
K93 Ubiquitination
S96 Phosphorylation
K142 Acetylation
K169 Acetylation
K193 Ubiquitination
T216 Phosphorylation
K249 Ubiquitination
R262 Methylation
T267 Phosphorylation
K272 Ubiquitination
S316 Phosphorylation
Y319 Phosphorylation
S339 Phosphorylation
K340 Ubiquitination
S341 Phosphorylation
S346 Phosphorylation
K349 Ubiquitination
Y376 Phosphorylation
T436 Phosphorylation
S440 Phosphorylation
C450 S-Nitrosylation
T472 Phosphorylation
T473 Phosphorylation
K480 Acetylation
S484 Phosphorylation
K492 Acetylation
K492 Ubiquitination
K495 Ubiquitination
K502 Acetylation
K502 Ubiquitination
S518 Phosphorylation
T519 Phosphorylation
T528 Phosphorylation
S537 Phosphorylation
K551 Ubiquitination
S567 Phosphorylation
Y596 Phosphorylation
S603 Phosphorylation
C604 S-Nitrosylation
K607 Ubiquitination
S630 Phosphorylation
Y632 Phosphorylation
T640 Phosphorylation
Y651 Phosphorylation
K657 Ubiquitination
S658 Phosphorylation
C660 S-Nitrosylation
K677 Acetylation
K681 Acetylation
K681 Ubiquitination
K697 Ubiquitination
Y704 Phosphorylation
S707 Phosphorylation
Y708 Phosphorylation
T709 Phosphorylation
S730 Phosphorylation
Y732 Phosphorylation
S740 Phosphorylation
K746 Ubiquitination
S755 Phosphorylation
C769 S-Nitrosylation
S789 Phosphorylation
S827 Phosphorylation
S833 Phosphorylation
S886 Phosphorylation
Y902 Phosphorylation
Y904 Phosphorylation
T907 Phosphorylation
T911 Phosphorylation
S932 Phosphorylation
S945 Phosphorylation
K946 Acetylation
R955 Methylation
K960 Ubiquitination
T965 Phosphorylation
T968 Phosphorylation
R969 Methylation
T980 Phosphorylation
S983 Phosphorylation
S985 Phosphorylation
T994 Phosphorylation
T997 Phosphorylation
Y1020 Phosphorylation
S1028 Phosphorylation
S1035 Phosphorylation
K1057 Ubiquitination
C1081 S-Nitrosylation
S1121 Phosphorylation
K1147 Ubiquitination
S1172 Phosphorylation
S1174 Phosphorylation
S1181 Phosphorylation
T1189 Phosphorylation
T1198 Phosphorylation
T1203 Phosphorylation
K1221 Ubiquitination
K1241 Ubiquitination
T1259 Phosphorylation
S1277 Phosphorylation
T1307 Phosphorylation
S1316 Phosphorylation
C1326 S-Nitrosylation
S1329 Phosphorylation
S1369 Phosphorylation
K1372 Ubiquitination
S1409 Phosphorylation
K1416 Acetylation
S1422 Phosphorylation
S1433 Phosphorylation
R1438 Methylation
S1442 Phosphorylation
S1447 Phosphorylation
S1448 Phosphorylation
K1449 Ubiquitination
S1505 Phosphorylation
K1508 Ubiquitination
S1528 Phosphorylation
S1529 Phosphorylation
Y1530 Phosphorylation
S1535 Phosphorylation
Y1576 Phosphorylation
T1579 Phosphorylation
T1585 Phosphorylation
S1602 Phosphorylation
T1609 Phosphorylation
K1616 Acetylation
C1617 S-Nitrosylation
K1641 Acetylation
K1641 Ubiquitination
Y1684 Phosphorylation
Y1687 Phosphorylation
Y1733 Phosphorylation
K1757 Ubiquitination
T1773 Phosphorylation
K1780 Ubiquitination
T1783 Phosphorylation
T1785 Phosphorylation
T1791 Phosphorylation
S1810 Phosphorylation
S1823 Phosphorylation
S1825 Phosphorylation
Y1827 Phosphorylation
Y1833 Phosphorylation
K1872 Ubiquitination
T1883 Phosphorylation
S1889 Phosphorylation
K1897 Acetylation
K1897 Ubiquitination
S1902 Phosphorylation
T1905 Phosphorylation
S1912 Phosphorylation
S1916 Phosphorylation
K1919 Ubiquitination
S1922 Phosphorylation
S1937 Phosphorylation
K1943 Ubiquitination
K1955 Ubiquitination
S1988 Phosphorylation
S1991 Phosphorylation
S1997 Phosphorylation
R2010 Methylation
S2013 Phosphorylation
T2017 Phosphorylation
S2021 Phosphorylation
S2036 Phosphorylation
K2044 Ubiquitination
K2058 Ubiquitination
S2060 Phosphorylation
Y2061 Phosphorylation
S2083 Phosphorylation
T2086 Phosphorylation
K2088 Ubiquitination
K2096 Ubiquitination
S2098 Phosphorylation
T2100 Phosphorylation
T2102 Phosphorylation
S2107 Phosphorylation
T2110 Phosphorylation
S2113 Phosphorylation
S2126 Phosphorylation
S2130 Phosphorylation
T2134 Phosphorylation
S2135 Phosphorylation
K2150 Ubiquitination
T2166 Phosphorylation
S2168 Phosphorylation
K2170 Acetylation
K2170 Ubiquitination
R2172 Methylation
T2177 Phosphorylation
S2179 Phosphorylation
R2197 Methylation
S2227 Phosphorylation
T2239 Phosphorylation
Y2260 Phosphorylation
S2274 Phosphorylation
Y2276 Phosphorylation
T2291 Phosphorylation
S2294 Phosphorylation
S2298 Phosphorylation
S2307 Phosphorylation
S2325 Phosphorylation
S2327 Phosphorylation
S2336 Phosphorylation
K2342 Acetylation
K2342 Ubiquitination
Y2343 Phosphorylation
S2364 Phosphorylation
S2369 Phosphorylation
S2403 Phosphorylation
T2410 Phosphorylation
S2418 Phosphorylation
S2425 Phosphorylation
T2434 Phosphorylation
T2443 Phosphorylation
S2453 Phosphorylation
S2465 Phosphorylation
K2468 Ubiquitination
T2472 Phosphorylation
S2478 Phosphorylation
S2481 Phosphorylation
T2485 Phosphorylation
S2487 Phosphorylation
S2492 Phosphorylation
T2494 Phosphorylation
T2500 Phosphorylation
C2501 S-Nitrosylation
Y2502 Phosphorylation
S2503 Phosphorylation
S2510 Phosphorylation
S2517 Phosphorylation
K2518 Ubiquitination
S2523 Phosphorylation
K2524 Acetylation
K2524 Ubiquitination
S2531 Phosphorylation
S2532 Phosphorylation

Research Backgrounds

Function:

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.

PTMs:

ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling.

Ubiquitination by a SCF-like complex containing ASB2 isoform 2 leads to proteasomal degradation which promotes muscle differentiation.

Subcellular Location:

Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cytoplasm>Myofibril>Sarcomere>Z line.
Note: In differentiating myotubes, isoform 1, isoform 2 and isoform 3 are localized diffusely throughout the cytoplasm with regions of enrichment at the longitudinal actin stress fiber. In differentiated tubes, isoform 1 is also detected within the Z-lines.

Cytoplasm>Cytoskeleton>Stress fiber.

Cytoplasm>Cytoskeleton>Stress fiber.

Cytoplasm>Cytoskeleton.
Note: Polarized at the periphery of myotubes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Subunit Structure:

Homodimer. Interacts with MICALL2 (By similarity). Interacts with RFLNA and RFLNB (By similarity). Isoform 1 interacts with FBLP1, FLNA, FLNC, GP1BA, INPPL1, ITGB1A, PSEN1 and PSEN2. Isoform 3 interacts with ITGB1A, ITGB1D, ITGB3 and ITGB6. Interacts with MYOT and MYOZ1. Interacts with HBV capsid protein.

Family&Domains:

Comprised of a NH2-terminal actin-binding domain, 24 internally homologous repeats and two hinge regions. Repeat 24 and the second hinge domain are important for dimer formation. The first hinge region prevents binding to ITGA and ITGB subunits.

Belongs to the filamin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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