DBT Antibody - #DF13569

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Product: DBT Antibody
Catalog: DF13569
Description: Rabbit polyclonal antibody to DBT
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 53kDa; 53kD(Calculated).
Uniprot: P11182
RRID: AB_2846588

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
DBT Antibody detects endogenous levels of total DBT.
RRID:
AB_2846588
Cite Format: Affinity Biosciences Cat# DF13569, RRID:AB_2846588.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCATE2; BCKAD E2 subunit; Branched chain acyltransferase, E2 component; Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease); Dihydrolipoamide branched chain transacylase; Dihydrolipoamide branched chain transacylase E2; Dihydrolipoyl transacylase; Dihydrolipoyllysine residue (2 methylpropanoyl)transferase; E2; E2 component of branched chain alpha keto acid dehydrogenase complex; E2B; Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex; Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b);

Immunogens

Immunogen:
Uniprot:

P11182(ODB2_HUMAN) >>Visit HPA database.

Gene(ID):
DBT(1629)
Sequence:
MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
86
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11182 As Substrate

Site PTM Type Enzyme
T56 Phosphorylation
T57 Phosphorylation
T80 Phosphorylation
T153 Phosphorylation
T171 Phosphorylation
K202 Ubiquitination
T213 Phosphorylation
S220 Phosphorylation
K289 Methylation
K295 Acetylation
T392 Phosphorylation
S403 Phosphorylation
K435 Acetylation
K435 Ubiquitination
K440 Acetylation
S447 Phosphorylation
S469 Phosphorylation

Research Backgrounds

Function:

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a 24-polypeptide structural core with octahedral symmetry.

Family&Domains:

Belongs to the 2-oxoacid dehydrogenase family.

Research Fields

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). A comprehensive analysis of the prognostic value and immune infiltration of low expression DBT in clear cell renal cell carcinoma. Frontiers in Pharmacology, 2022 (PubMed: 36299888) [IF=5.6]

Application: WB    Species: Human    Sample: HK-2 cells

FIGURE 2 DBT protein expression is low in ccRCC. (A) The histogram obtained from the analysis of data from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) on the UALCAN website shows significantly lower expression of the DBT protein in ccRCC tissues versus normal tissues (p = 1.31015316430268E-57). (B) The results of western blotting of A498, 786-O, ACHN, Caki-1, and OSRC-2 cell lysates showed lower protein expression of DBT in ccRCC cells compared to HK-2 cells, and the histogram quantifying the results also indicated a statistically significant difference. (C) Similarly, DBT protein expression was lower in renal clear cell carcinoma tissues than in matched pairs of paraneoplastic normal tissues, and the histogram quantification confirmed this finding. (D) Immunohistochemical results from the HPA database showed that DBT expression in ccRCC was mainly in the nucleus, whereas in normal renal tissue, it was in normal tubular epithelial cells. (E) This result was also validated by the immunohistochemical results of 2 pairs of ccRCC tissues from our study centre.

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