DNA Polymerase theta Antibody | Affinity Biosciences

Product:	DNA Polymerase theta Antibody
Catalog:	DF13563
Description:	Rabbit polyclonal antibody to DNA Polymerase theta
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	289kDa,150kD; 290kD(Calculated).
Uniprot:	O75417
RRID:	AB_2846582

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

DNA Polymerase theta Antibody detects endogenous levels of total DNA Polymerase theta.

RRID:

AB_2846582
Cite Format: Affinity Biosciences Cat# DF13563, RRID:AB_2846582.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp781A0112; DNA polymerase eta; DNA polymerase theta; DPOLQ_HUMAN; POLQ; Polymerase (DNA directed) theta; PRO0327;

Immunogens

Immunogen:

Uniprot:

O75417(DPOLQ_HUMAN) >>Visit HPA database.

Gene(ID):

POLQ(10721)

Expression:

O75417 DPOLQ_HUMAN:

Highly expressed in testis.

Sequence:

O75417 DPOLQ_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

PTMs - O75417 As Substrate

O75417

Site	PTM Type	Source
S15	Phosphorylation	Uniprot
S23	Phosphorylation	Uniprot
Y84	Phosphorylation	Uniprot
T117	Phosphorylation	Uniprot
Y171	Phosphorylation	Uniprot
S176	Phosphorylation	Uniprot
K308	Ubiquitination	Uniprot
K475	Ubiquitination	Uniprot
K484	Ubiquitination	Uniprot
K497	Ubiquitination	Uniprot
K513	Ubiquitination	Uniprot
T557	Phosphorylation	Uniprot
S750	Phosphorylation	Uniprot
K844	Ubiquitination	Uniprot
S925	Phosphorylation	Uniprot
K928	Ubiquitination	Uniprot
K950	Ubiquitination	Uniprot
S952	Phosphorylation	Uniprot
K961	Ubiquitination	Uniprot
K990	Acetylation	Uniprot
K998	Acetylation	Uniprot
K1000	Acetylation	Uniprot
K1009	Acetylation	Uniprot
T1018	Phosphorylation	Uniprot
S1020	Phosphorylation	Uniprot
K1034	Ubiquitination	Uniprot
K1248	Ubiquitination	Uniprot
S1260	Phosphorylation	Uniprot
K1277	Ubiquitination	Uniprot
S1289	Phosphorylation	Uniprot
K1294	Ubiquitination	Uniprot
T1295	Phosphorylation	Uniprot
T1299	Phosphorylation	Uniprot
T1300	Phosphorylation	Uniprot
K1341	Ubiquitination	Uniprot
K1345	Ubiquitination	Uniprot
T1347	Phosphorylation	Uniprot
S1363	Phosphorylation	Uniprot
S1414	Phosphorylation	Uniprot
S1587	Phosphorylation	Uniprot
S1651	Phosphorylation	Uniprot
S1660	Phosphorylation	Uniprot
S1667	Phosphorylation	Uniprot
K1671	Ubiquitination	Uniprot
S1683	Phosphorylation	Uniprot
T1687	Phosphorylation	Uniprot
S1745	Phosphorylation	Uniprot
K1866	Ubiquitination	Uniprot
T1940	Phosphorylation	Uniprot
T2161	Phosphorylation	Uniprot
T2179	Phosphorylation	Uniprot
S2267	Phosphorylation	Uniprot
S2311	Phosphorylation	Uniprot
S2313	Phosphorylation	Uniprot
S2397	Phosphorylation	Uniprot
Y2412	Phosphorylation	Uniprot
K2417	Ubiquitination	Uniprot
T2430	Phosphorylation	Uniprot
T2471	Phosphorylation	Uniprot
S2511	Phosphorylation	Uniprot

Research Backgrounds

O75417_DPOLQ_HUMAN

Function:

DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends. POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates. Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining. Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).

Subcellular Location:

Nucleus. Chromosome.
Note: Enriched in chromatin in response to ultaviolet (UV) light (PubMed:25642963). Binds to chromatin during early G1 (PubMed:24989122).

Tissue Specificity:

Highly expressed in testis.

Subunit Structure:

Homomultimer; forms dimers and multimers. Interacts with RAD51. Interacts with ORC2 and ORC4.

Family&Domains:

The loop 2 region is involved in the binding of the 2 ends of resected double-strand breaks and homomultimerization.

Belongs to the DNA polymerase type-A family.

References

1). ALDH1A1 promotes PARP inhibitor resistance by enhancing retinoic acid receptor-mediated DNA polymerase θ expression. npj Precision Oncology, 2023 (PubMed: 37429899) [IF=7.9]

Application: WB Species: Human Sample: PEO1 and OVCAR3 cells

Fig. 1 ALDH1A1 enhances Polθ expression. a Volcano plot depicting differentially expressed MMEJ-related genes in ALDH+ vs ALDH- cells isolated from SKOV3 cells. b Volcano plot depicting differentially expressed MMEJ-related genes in siALDH1A1 transfected vs siControl transfected PEO1 cells. c The POLQ mRNA level is higher in ALDH-br cells than that in ALDH-dim cells isolated from PEO1 and OVCAR3 cells. d Overexpression of ALDH1A1 increased the mRNA level of POLQ. e Overexpression of ALDH1A1 increased the protein level of Polθ. f Knockdown of ALDH1A1 decreased the mRNA level of POLQ. g Knockdown of ALDH1A1 decreased the protein level of Polθ. h Inhibition of the activity of ALDH1A1 with NCT-505 reduced the expression of Polθ. Measurements were taken from distinct samples. N = 3, bar: s.d., **P

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DNA Polymerase theta Antibody - #DF13563