Product: DNA Polymerase theta Antibody
Catalog: DF13563
Description: Rabbit polyclonal antibody to DNA Polymerase theta
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 289kDa,150kD; 290kD(Calculated).
Uniprot: O75417
RRID: AB_2846582

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
DNA Polymerase theta Antibody detects endogenous levels of total DNA Polymerase theta.
RRID:
AB_2846582
Cite Format: Affinity Biosciences Cat# DF13563, RRID:AB_2846582.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp781A0112; DNA polymerase eta; DNA polymerase theta; DPOLQ_HUMAN; POLQ; Polymerase (DNA directed) theta; PRO0327;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O75417 DPOLQ_HUMAN:

Highly expressed in testis.

Sequence:
MNLLRRSGKRRRSESGSDSFSGSGGDSSASPQFLSGSVLSPPPGLGRCLKAAAAGECKPTVPDYERDKLLLANWGLPKAVLEKYHSFGVKKMFEWQAECLLLGQVLEGKNLVYSAPTSAGKTLVAELLILKRVLEMRKKALFILPFVSVAKEKKYYLQSLFQEVGIKVDGYMGSTSPSRHFSSLDIAVCTIERANGLINRLIEENKMDLLGMVVVDELHMLGDSHRGYLLELLLTKICYITRKSASCQADLASSLSNAVQIVGMSATLPNLELVASWLNAELYHTDFRPVPLLESVKVGNSIYDSSMKLVREFEPMLQVKGDEDHVVSLCYETICDNHSVLLFCPSKKWCEKLADIIAREFYNLHHQAEGLVKPSECPPVILEQKELLEVMDQLRRLPSGLDSVLQKTVPWGVAFHHAGLTFEERDIIEGAFRQGLIRVLAATSTLSSGVNLPARRVIIRTPIFGGRPLDILTYKQMVGRAGRKGVDTVGESILICKNSEKSKGIALLQGSLKPVRSCLQRREGEEVTGSMIRAILEIIVGGVASTSQDMHTYAACTFLAASMKEGKQGIQRNQESVQLGAIEACVMWLLENEFIQSTEASDGTEGKVYHPTHLGSATLSSSLSPADTLDIFADLQRAMKGFVLENDLHILYLVTPMFEDWTTIDWYRFFCLWEKLPTSMKRVAELVGVEEGFLARCVKGKVVARTERQHRQMAIHKRFFTSLVLLDLISEVPLREINQKYGCNRGQIQSLQQSAAVYAGMITVFSNRLGWHNMELLLSQFQKRLTFGIQRELCDLVRVSLLNAQRARVLYASGFHTVADLARANIVEVEVILKNAVPFKSARKAVDEEEEAVEERRNMRTIWVTGRKGLTEREAAALIVEEARMILQQDLVEMGVQWNPCALLHSSTCSLTHSESEVKEHTFISQTKSSYKKLTSKNKSNTIFSDSYIKHSPNIVQDLNKSREHTSSFNCNFQNGNQEHQTCSIFRARKRASLDINKEKPGASQNEGKTSDKKVVQTFSQKTKKAPLNFNSEKMSRSFRSWKRRKHLKRSRDSSPLKDSGACRIHLQGQTLSNPSLCEDPFTLDEKKTEFRNSGPFAKNVSLSGKEKDNKTSFPLQIKQNCSWNITLTNDNFVEHIVTGSQSKNVTCQATSVVSEKGRGVAVEAEKINEVLIQNGSKNQNVYMKHHDIHPINQYLRKQSHEQTSTITKQKNIIERQMPCEAVSSYINRDSNVTINCERIKLNTEENKPSHFQALGDDISRTVIPSEVLPSAGAFSKSEGQHENFLNISRLQEKTGTYTTNKTKNNHVSDLGLVLCDFEDSFYLDTQSEKIIQQMATENAKLGAKDTNLAAGIMQKSLVQQNSMNSFQKECHIPFPAEQHPLGATKIDHLDLKTVGTMKQSSDSHGVDILTPESPIFHSPILLEENGLFLKKNEVSVTDSQLNSFLQGYQTQETVKPVILLIPQKRTPTGVEGECLPVPETSLNMSDSLLFDSFSDDYLVKEQLPDMQMKEPLPSEVTSNHFSDSLCLQEDLIKKSNVNENQDTHQQLTCSNDESIIFSEMDSVQMVEALDNVDIFPVQEKNHTVVSPRALELSDPVLDEHHQGDQDGGDQDERAEKSKLTGTRQNHSFIWSGASFDLSPGLQRILDKVSSPLENEKLKSMTINFSSLNRKNTELNEEQEVISNLETKQVQGISFSSNNEVKSKIEMLENNANHDETSSLLPRKESNIVDDNGLIPPTPIPTSASKLTFPGILETPVNPWKTNNVLQPGESYLFGSPSDIKNHDLSPGSRNGFKDNSPISDTSFSLQLSQDGLQLTPASSSSESLSIIDVASDQNLFQTFIKEWRCKKRFSISLACEKIRSLTSSKTATIGSRFKQASSPQEIPIRDDGFPIKGCDDTLVVGLAVCWGGRDAYYFSLQKEQKHSEISASLVPPSLDPSLTLKDRMWYLQSCLRKESDKECSVVIYDFIQSYKILLLSCGISLEQSYEDPKVACWLLDPDSQEPTLHSIVTSFLPHELPLLEGMETSQGIQSLGLNAGSEHSGRYRASVESILIFNSMNQLNSLLQKENLQDVFRKVEMPSQYCLALLELNGIGFSTAECESQKHIMQAKLDAIETQAYQLAGHSFSFTSSDDIAEVLFLELKLPPNREMKNQGSKKTLGSTRRGIDNGRKLRLGRQFSTSKDVLNKLKALHPLPGLILEWRRITNAITKVVFPLQREKCLNPFLGMERIYPVSQSHTATGRITFTEPNIQNVPRDFEIKMPTLVGESPPSQAVGKGLLPMGRGKYKKGFSVNPRCQAQMEERAADRGMPFSISMRHAFVPFPGGSILAADYSQLELRILAHLSHDRRLIQVLNTGADVFRSIAAEWKMIEPESVGDDLRQQAKQICYGIIYGMGAKSLGEQMGIKENDAACYIDSFKSRYTGINQFMTETVKNCKRDGFVQTILGRRRYLPGIKDNNPYRKAHAERQAINTIVQGSAADIVKIATVNIQKQLETFHSTFKSHGHREGMLQSDQTGLSRKRKLQGMFCPIRGGFFILQLHDELLYEVAEEDVVQVAQIVKNEMESAVKLSVKLKVKVKIGASWGELKDFDV

PTMs - O75417 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
S23 Phosphorylation
Y84 Phosphorylation
T117 Phosphorylation
Y171 Phosphorylation
S176 Phosphorylation
K308 Ubiquitination
K475 Ubiquitination
K484 Ubiquitination
K497 Ubiquitination
K513 Ubiquitination
T557 Phosphorylation
S750 Phosphorylation
K844 Ubiquitination
S925 Phosphorylation
K928 Ubiquitination
K950 Ubiquitination
S952 Phosphorylation
K961 Ubiquitination
K990 Acetylation
K998 Acetylation
K1000 Acetylation
K1009 Acetylation
T1018 Phosphorylation
S1020 Phosphorylation
K1034 Ubiquitination
K1248 Ubiquitination
S1260 Phosphorylation
K1277 Ubiquitination
S1289 Phosphorylation
K1294 Ubiquitination
T1295 Phosphorylation
T1299 Phosphorylation
T1300 Phosphorylation
K1341 Ubiquitination
K1345 Ubiquitination
T1347 Phosphorylation
S1363 Phosphorylation
S1414 Phosphorylation
S1587 Phosphorylation
S1651 Phosphorylation
S1660 Phosphorylation
S1667 Phosphorylation
K1671 Ubiquitination
S1683 Phosphorylation
T1687 Phosphorylation
S1745 Phosphorylation
K1866 Ubiquitination
T1940 Phosphorylation
T2161 Phosphorylation
T2179 Phosphorylation
S2267 Phosphorylation
S2311 Phosphorylation
S2313 Phosphorylation
S2397 Phosphorylation
Y2412 Phosphorylation
K2417 Ubiquitination
T2430 Phosphorylation
T2471 Phosphorylation
S2511 Phosphorylation

Research Backgrounds

Function:

DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends. POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ. The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates. Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining. Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).

Subcellular Location:

Nucleus. Chromosome.
Note: Enriched in chromatin in response to ultaviolet (UV) light (PubMed:25642963). Binds to chromatin during early G1 (PubMed:24989122).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis.

Subunit Structure:

Homomultimer; forms dimers and multimers. Interacts with RAD51. Interacts with ORC2 and ORC4.

Family&Domains:

The loop 2 region is involved in the binding of the 2 ends of resected double-strand breaks and homomultimerization.

Belongs to the DNA polymerase type-A family.

References

1). ALDH1A1 promotes PARP inhibitor resistance by enhancing retinoic acid receptor-mediated DNA polymerase θ expression. npj Precision Oncology, 2023 (PubMed: 37429899) [IF=7.9]

Application: WB    Species: Human    Sample: PEO1 and OVCAR3 cells

Fig. 1 ALDH1A1 enhances Polθ expression. a Volcano plot depicting differentially expressed MMEJ-related genes in ALDH+ vs ALDH- cells isolated from SKOV3 cells. b Volcano plot depicting differentially expressed MMEJ-related genes in siALDH1A1 transfected vs siControl transfected PEO1 cells. c The POLQ mRNA level is higher in ALDH-br cells than that in ALDH-dim cells isolated from PEO1 and OVCAR3 cells. d Overexpression of ALDH1A1 increased the mRNA level of POLQ. e Overexpression of ALDH1A1 increased the protein level of Polθ. f Knockdown of ALDH1A1 decreased the mRNA level of POLQ. g Knockdown of ALDH1A1 decreased the protein level of Polθ. h Inhibition of the activity of ALDH1A1 with NCT-505 reduced the expression of Polθ. Measurements were taken from distinct samples. N = 3, bar: s.d., **P 

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