Steroid sulfatase Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Human Placental, using Steroid sulfatase Antibody.

Product:	Steroid sulfatase Antibody
Catalog:	DF13557
Description:	Rabbit polyclonal antibody to Steroid sulfatase
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Bovine, Sheep
Mol.Wt.:	65kDa; 65kD(Calculated).
Uniprot:	P08842
RRID:	AB_2846576

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Bovine(100%), Sheep(88%)

Clonality:

Polyclonal

Specificity:

Steroid sulfatase Antibody detects endogenous levels of total Steroid sulfatase.

RRID:

AB_2846576
Cite Format: Affinity Biosciences Cat# DF13557, RRID:AB_2846576.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ARSC; ARSC1; Arylsulfatase C; Arylsulfatase C isozyme S; ASC; EC 3.1.6.2; ES; Estrone sulfatase; SSDD; Steroid sulfatase (microsomal); Steroid sulfatase (microsomal) arylsulfatase C isozyme S; Steroid sulfatase; Steryl sulfatase; Steryl sulfatase precursor; Steryl sulfate sulfohydrolase; Steryl-sulfatase; Steryl-sulfate sulfohydrolase; STS; STS_HUMAN;

Immunogens

Immunogen:

Uniprot:

P08842(STS_HUMAN) >>Visit HPA database.

Gene(ID):

STS(412)

Sequence:

P08842 STS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPLRKMKIPFLLLFFLWEAESHAASRPNIILVMADDLGIGDPGCYGNKTIRTPNIDRLASGGVKLTQHLAASPLCTPSRAAFMTGRYPVRSGMASWSRTGVFLFTASSGGLPTDEITFAKLLKDQGYSTALIGKWHLGMSCHSKTDFCHHPLHHGFNYFYGISLTNLRDCKPGEGSVFTTGFKRLVFLPLQIVGVTLLTLAALNCLGLLHVPLGVFFSLLFLAALILTLFLGFLHYFRPLNCFMMRNYEIIQQPMSYDNLTQRLTVEAAQFIQRNTETPFLLVLSYLHVHTALFSSKDFAGKSQHGVYGDAVEEMDWSVGQILNLLDELRLANDTLIYFTSDQGAHVEEVSSKGEIHGGSNGIYKGGKANNWEGGIRVPGILRWPRVIQAGQKIDEPTSNMDIFPTVAKLAGAPLPEDRIIDGRDLMPLLEGKSQRSDHEFLFHYCNAYLNAVRWHPQNSTSIWKAFFFTPNFNPVGSNGCFATHVCFCFGSYVTHHDPPLLFDISKDPRERNPLTPASEPRFYEILKVMQEAADRHTQTLPEVPDQFSWNNFLWKPWLQLCCPSTGLSCQCDREKQDKRLSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

Pig

Dog

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08842 As Substrate

P08842

Site	PTM Type	Source
N47	N-Glycosylation	Uniprot
K123	Ubiquitination	Uniprot
N259	N-Glycosylation	Uniprot
K393	Ubiquitination	Uniprot
K409	Ubiquitination	Uniprot

Research Backgrounds

P08842_STS_HUMAN

Function:

Catalyzes the conversion of sulfated steroid precursors, such as dehydroepiandrosterone sulfate (DHEA-S) and estrone sulfate to the free steroid.

PTMs:

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

Subcellular Location:

Cytoplasmic vesicle>Secretory vesicle>Microneme membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the sulfatase family.

Research Fields

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Steroid sulfatase Antibody - #DF13557