SERBP1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from RT4, using SERBP1 Antibody.

Figure 1 SERPINE1 is upregulated in hemin-treated HT22 cells.

Product:	SERBP1 Antibody
Catalog:	DF13553
Description:	Rabbit polyclonal antibody to SERBP1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	44kDa; 45kD(Calculated).
Uniprot:	Q8NC51
RRID:	AB_2846572

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

SERBP1 Antibody detects endogenous levels of total SERBP1.

RRID:

AB_2846572
Cite Format: Affinity Biosciences Cat# DF13553, RRID:AB_2846572.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CGI 55; CHD3IP; Chromodomain helicase DNA binding protein 3 interacting protein; DKFZp564M2423; FLJ90489; HABP4L; PAI 1 mRNA binding protein; PAI RBP1; PAI-RBP1; PAI1 RNA-binding protein 1; PAIRB_HUMAN; PAIRBP1; Plasminogen activator inhibitor 1 RNA binding protein; Plasminogen activator inhibitor 1 RNA-binding protein; Serbp1; SERPINE1 mRNA binding protein 1; SERPINE1 mRNA-binding protein 1;

Immunogens

Immunogen:

Uniprot:

Q8NC51(PAIRB_HUMAN) >>Visit HPA database.

Gene(ID):

SERBP1(26135)

Expression:

Q8NC51 PAIRB_HUMAN:

Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.

Sequence:

Q8NC51 PAIRB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPGHLQEGFGCVVTNRFDQLFDDESDPFEVLKAAENKKKEAGGGGVGGPGAKSAAQAAAQTNSNAAGKQLRKESQKDRKNPLPPSVGVVDKKEETQPPVALKKEGIRRVGRRPDQQLQGEGKIIDRRPERRPPRERRFEKPLEEKGEGGEFSVDRPIIDRPIRGRGGLGRGRGGRGRGMGRGDGFDSRGKREFDRHSGSDRSSFSHYSGLKHEDKRGGSGSHNWGTVKDELTESPKYIQKQISYNYSDLDQSNVTEETPEGEEHHPVADTENKENEVEEVKEEGPKEMTLDEWKAIQNKDRAKVEFNIRKPNEGADGQWKKGFVLHKSKSEEAHAEDSVMDHHFRKPANDITSQLEINFGDLGRPGRGGRGGRGGRGRGGRPNRGSRTDKSSASAPDVDDPEAFPALA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Bovine

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NC51 As Substrate

Q8NC51

Site	PTM Type	Source
C11	S-Nitrosylation	Uniprot
S25	Phosphorylation	Uniprot
K32	Ubiquitination	Uniprot
K39	Ubiquitination	Uniprot
K52	Sumoylation	Uniprot
K52	Ubiquitination	Uniprot
S53	Phosphorylation	Uniprot
T61	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
K68	Acetylation	Uniprot
K68	Ubiquitination	Uniprot
S85	Phosphorylation	Uniprot
K92	Ubiquitination	Uniprot
T95	Phosphorylation	Uniprot
K102	Acetylation	Uniprot
K102	Sumoylation	Uniprot
K102	Ubiquitination	Uniprot
K122	Acetylation	Uniprot
K122	Ubiquitination	Uniprot
R126	Methylation	Uniprot
K140	Acetylation	Uniprot
K140	Ubiquitination	Uniprot
S152	Phosphorylation	Uniprot
R155	Methylation	Uniprot
R160	Methylation	Uniprot
R163	Methylation	Uniprot
R165	Methylation	Uniprot
R177	Methylation	Uniprot
R181	Methylation	Uniprot
R188	Methylation	Uniprot
R195	Methylation	Uniprot
S197	Phosphorylation	Uniprot
R201	Methylation	Uniprot
S202	Phosphorylation	Uniprot
S203	Phosphorylation	Uniprot
S205	Phosphorylation	Uniprot
Y207	Phosphorylation	Uniprot
S208	Phosphorylation	Uniprot
K211	Acetylation	Uniprot
K211	Ubiquitination	Uniprot
R216	Methylation	Uniprot
S219	Phosphorylation	Uniprot
S221	Phosphorylation	Uniprot
K228	Sumoylation	Uniprot
K228	Ubiquitination	Uniprot
T232	Phosphorylation	Uniprot
S234	Phosphorylation	Uniprot
K236	Ubiquitination	Uniprot
Y237	Phosphorylation	Uniprot
K240	Ubiquitination	Uniprot
S243	Phosphorylation	Uniprot
Y244	Phosphorylation	Uniprot
Y246	Phosphorylation	Uniprot
S247	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
T255	Phosphorylation	Uniprot
T258	Phosphorylation	Uniprot
K281	Sumoylation	Uniprot
K281	Ubiquitination	Uniprot
K286	Ubiquitination	Uniprot
K294	Ubiquitination	Uniprot
K303	Ubiquitination	Uniprot
K310	Ubiquitination	Uniprot
K320	Ubiquitination	Uniprot
K321	Acetylation	Uniprot
K321	Ubiquitination	Uniprot
K327	Methylation	Uniprot
K327	Ubiquitination	Uniprot
S328	Phosphorylation	Uniprot
S330	Phosphorylation	Uniprot
S338	Phosphorylation	Uniprot
K346	Ubiquitination	Uniprot
S353	Phosphorylation	Uniprot
R364	Methylation	Uniprot
R367	Methylation	Uniprot
R370	Methylation	Uniprot
R373	Methylation	Uniprot
T388	Phosphorylation	Uniprot
K390	Ubiquitination	Uniprot
S391	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot
S394	Phosphorylation	Uniprot

Research Backgrounds

Q8NC51_PAIRB_HUMAN

Function:

May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay. Seems to play a role in PML-nuclear bodies formation.

Subcellular Location:

Cytoplasm. Nucleus. Cytoplasm>Perinuclear region.

Tissue Specificity:

Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain.

Subunit Structure:

Interacts with SPIN1 (By similarity). Interacts with CHD3 and TDRD3. Interacts with ZDHHC17 (via ANK repeats).

References

1). Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling. Molecular Medicine Reports, 2020 (PubMed: 33174043) [IF=3.4]

Application: WB Species: Mice Sample: HEI-OC1 cells

Figure 5. FPKM values and expression of several DEGs in the three groups. (A-D) FPKM values of several random selected up- or downregulated DEGs with typical expression profiling in response to treatment with cisplatin alone or combined with Tet. (E) Expression of several protein products of DEGs. *P<0.05, vs. control. #P<0.05 vs. Cisplatin. The differences were analyzed by one-way ANOVA followed by Tukey's post hoc test. DEG, differentially expressed gene; FKPM, fragments per kilobase of exon model per million reads mapped.

2). Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy. Free radical research, 2023 (PubMed: 37897414) [IF=3.3]

3). TGF-β1-Mediated Activation of SERPINE1 is Involved in Hemin-Induced Apoptotic and Inflammatory Injury in HT22 Cells. Neuropsychiatric Disease and Treatment, 2021 (PubMed: 33603380) [IF=3.2]

Application: WB Species: Mice Sample: HT22 cells

Figure 1 SERPINE1 is upregulated in hemin-treated HT22 cells. (A) HT22 cells were treated with different doses (0, 10, 20, 40, 60, 80, 100, and 120 μM) of hemin for 24 h and then subjected to MTT assay. (B) HT22 cells were treated with 100 μM of hemin for 24 h and then assayed for SERPINE1 expression using qRT-PCR assay. (C) Representative image of Western blot analysis and quantification of SERPINE1 expression in HT22 cells stimulated with hemin. *p < 0.05, **p <0.01, and ***p <0.001.

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SERBP1 Antibody - #DF13553