GLUT5 Antibody - #DF13545

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Product: GLUT5 Antibody
Catalog: DF13545
Description: Rabbit polyclonal antibody to GLUT5
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Dog
Mol.Wt.: 50~70kDa; 55kD(Calculated).
Uniprot: P22732
RRID: AB_2846564

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Bovine(88%), Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Glucose Transporter 5 GLUT5 Antibody detects endogenous levels of total Glucose Transporter 5 GLUT5.
RRID:
AB_2846564
Cite Format: Affinity Biosciences Cat# DF13545, RRID:AB_2846564.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Facilitated glucose transporter member 5; Fructose transporter; Glucose transporte, kidney; Glucose Transporter 5 GLUT5; glucose transporter like protein 5; Glucose transporter type 5; Glucose transporter type 5 small intestine; GLUT 5; GLUT-5; GLUT5; GTR5_HUMAN; SLC 2A5; SLC2A5; small intestine; solute carrier family 2 (facilitated glucose/fructose transporter), member 5; Solute carrier family 2;

Immunogens

Immunogen:
Uniprot:

P22732(GTR5_HUMAN) >>Visit HPA database.

Gene(ID):
SLC2A5(6518)
Expression:
P22732 GTR5_HUMAN:

Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level) (PubMed:7619085). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

Sequence:
MEQQDQSMKEGRLTLVLALATLIAAFGSSFQYGYNVAAVNSPALLMQQFYNETYYGRTGEFMEDFPLTLLWSVTVSMFPFGGFIGSLLVGPLVNKFGRKGALLFNNIFSIVPAILMGCSRVATSFELIIISRLLVGICAGVSSNVVPMYLGELAPKNLRGALGVVPQLFITVGILVAQIFGLRNLLANVDGWPILLGLTGVPAALQLLLLPFFPESPRYLLIQKKDEAAAKKALQTLRGWDSVDREVAEIRQEDEAEKAAGFISVLKLFRMRSLRWQLLSIIVLMGGQQLSGVNAIYYYADQIYLSAGVPEEHVQYVTAGTGAVNVVMTFCAVFVVELLGRRLLLLLGFSICLIACCVLTAALALQDTVSWMPYISIVCVISYVIGHALGPSPIPALLITEIFLQSSRPSAFMVGGSVHWLSNFTVGLIFPFIQEGLGPYSFIVFAVICLLTTIYIFLIVPETKAKTFIEINQIFTKMNKVSEVYPEKEELKELPPVTSEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
88
Bovine
88
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P22732 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K9 Ubiquitination
R120 Methylation
T199 Phosphorylation
K477 Ubiquitination
K488 Ubiquitination
K492 Ubiquitination

Research Backgrounds

Function:

Functions as a fructose transporter that has only low activity with other monosaccharides. Can mediate the uptake of 2-deoxyglucose, but with low efficiency. Essential for fructose uptake in the small intestine (By similarity). Plays a role in the regulation of salt uptake and blood pressure in response to dietary fructose (By similarity). Required for the development of high blood pressure in response to high dietary fructose intake (By similarity).

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cell membrane>Sarcolemma.
Note: Localized on the apical membrane of jejunum villi, but also on lateral plasma membranes of the villi. Transport to the cell membrane is dependent on RAB11A.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in skeletal muscle, and in jejunum brush border membrane and basolateral membrane (at protein level). Expressed in small intestine, and at much lower levels in kidney, skeletal muscle, and adipose tissue.

Family&Domains:

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

Research Fields

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

References

1). Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice. JCI Insight, 2022 (PubMed: 35077394) [IF=8.0]

Application: WB    Species: Mice    Sample: Hippocampal tissues

Figure 5 RH reduced brain GLUT3-mediated glucose uptake in STZ-induced APP/PS1-DM mice. (A) Representative PET/CT images showing in vivo brain 18F-FDG uptake (coronal, sagittal, and transaxial sections, n = 6 mice for each group). Cor, Cortex; Hip, hippocampus; Mid, midbrain; HPOA, hypothalamus; BS, brain stem; Cer, cerebellum; OB, Olfactory Bulb; BF, basal forebrain; Tha, thalamus; SC, superior colliculi; CG, central gray; Str, striatum. (B) Quantification for SUV of 18F-FDG in brain region (n = 6 for mice for each group). (C) Western blot and quantitation for GLUT1, GLUT2, GLUT3, GLUT4, GLUT5, SGLT1, and SGLT2 in hippocampal homogenates (n = 3 mice for each group). The data are expressed as the mean ± SEM. Statistical significance was assessed using unpaired Student’s t test. *P < 0.05 and **P < 0.01, APP/PS1-DM versus APP/PS1; #P < 0.05 and ##P < 0.01, APP/PS1-DM-RH versus APP/PS1-DM; WT versus APP/PS1; NS, no significant difference.
2). The mTORC1/eIF4E/HIF-1α pathway mediates glycolysis to support brain hypoxia resistance in the Gansu zokor, Eospalax cansus. Frontiers in Physiology, 2021 (PubMed: 33708138) [IF=4.0]

Application: WB    Species: Rat    Sample: brain tissues

FIGURE 4 Translation level analysis of glycolysis-related transporters and enzymes in Sprague-Dawley rat and Gansu zokor. GLUT1 glucose transporter (A), PFK (B), GLUT5 glucose transporter (C), and KHK (D) protein expression. Representative images of at least three separate blots below each bar graph showing expression of the proteins. All data are presented as mean ± SEM. Statistical symbols: #P < 0.05 and ##P < 0.01 between two species under the same oxygen treatment; *P < 0.05 and **P < 0.01 between different oxygen treatments in the same species.

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