IL1RAP Antibody - #DF13533

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Product: IL1RAP Antibody
Catalog: DF13533
Description: Rabbit polyclonal antibody to IL1RAP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 65kDa; 65kD(Calculated).
Uniprot: Q9NPH3
RRID: AB_2846552

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(86%)
Clonality:
Polyclonal
Specificity:
IL1RAP Antibody detects endogenous levels of total IL1RAP.
RRID:
AB_2846552
Cite Format: Affinity Biosciences Cat# DF13533, RRID:AB_2846552.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C3orf13; FLJ37788; IL 1 receptor accessory protein; IL 1R 3; IL 1R3; IL 1RAcP; IL 1RAP; IL-1 receptor accessory protein; IL-1R-3; IL-1R3; IL-1RAcP; IL-1RAP; IL1 receptor accessory protein; IL1AP_HUMAN; IL1R3; IL1RAcP; Il1rap; Interleukin 1 Accessory Protein; interleukin 1 receptor 3; Interleukin 1 receptor accessory protein; interleukin 1 receptor accessory protein beta; Interleukin-1 receptor 3; Interleukin-1 receptor accessory protein;

Immunogens

Immunogen:
Uniprot:

Q9NPH3(IL1AP_HUMAN) >>Visit HPA database.

Gene(ID):
IL1RAP(3556)
Expression:
Q9NPH3 IL1AP_HUMAN:

Detected in liver, skin, placenta, thymus and lung. Isoform 4 is predominantly expressed in brain. Overexpressed on candidate chronic myeloid leukemia (CML) stem cells, hematopoietic stem cells and mononuclear cells of patients with acute myeloid leukemia (AML). Overexpressed in patients with chronic obstructive pulmonary disease (COPD). Expressed in T-helper 1 (Th1) and T-helper 2 (Th2) cell subsets (PubMed:10653850).

Sequence:
MTLLWCVVSLYFYGILQSDASERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIALISNNGNYTCVVTYPENGRTFHLTRTLTVKVVGSPKNAVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDITIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAAKVKQKVPAPRYTVELACGFGATVLLVVILIVVYHVYWLEMVLFYRAHFGTDETILDGKEYDIYVSYARNAEEEEFVLLTLRGVLENEFGYKLCIFDRDSLPGGIVTDETLSFIQKSRRLLVVLSPNYVLQGTQALLELKAGLENMASRGNINVILVQYKAVKETKVKELKRAKTVLTVIKWKGEKSKYPQGRFWKQLQVAMPVKKSPRRSSSDEQGLSYSSLKNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Chicken
86
Pig
0
Bovine
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NPH3 As Substrate

Site PTM Type Enzyme
T104 Phosphorylation
N107 N-Glycosylation
N111 N-Glycosylation
Y112 Phosphorylation
T113 Phosphorylation
N118 N-Glycosylation
K134 Acetylation
K143 Acetylation
N209 N-Glycosylation
T293 Phosphorylation
S303 Phosphorylation
S305 Phosphorylation
T509 Phosphorylation
K518 Ubiquitination
K532 Ubiquitination
S551 Phosphorylation
S555 Phosphorylation
S556 Phosphorylation
S557 Phosphorylation
S563 Phosphorylation
Y564 Phosphorylation
S565 Phosphorylation
S566 Phosphorylation
K568 Ubiquitination

Research Backgrounds

Function:

Coreceptor for IL1RL2 in the IL-36 signaling system (By similarity). Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (By similarity). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (Probable).

Associates with secreted ligand-bound IL1R2 and increases the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Enhances the ability of secreted IL1R1 to inhibit IL-33 signaling (By similarity).

Unable to mediate canonical IL-1 signaling. Required for Src phosphorylation by IL1B. May be involved in IL1B-potentiated NMDA-induced calcium influx in neurons (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Secreted.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in liver, skin, placenta, thymus and lung. Isoform 4 is predominantly expressed in brain. Overexpressed on candidate chronic myeloid leukemia (CML) stem cells, hematopoietic stem cells and mononuclear cells of patients with acute myeloid leukemia (AML). Overexpressed in patients with chronic obstructive pulmonary disease (COPD). Expressed in T-helper 1 (Th1) and T-helper 2 (Th2) cell subsets.

Subunit Structure:

The interleukin-36 receptor complex is a heterodimer of IL1RL2 and IL1RAP; the association is inhibited by IL36RN (By similarity). The interleukin-1 receptor complex is a heterodimer of IL1R1 and IL1RAP. Associates with IL1R2 to form a non-signaling interleukin-1 receptor complex. Isoform 4 interacts with IL1R1 in an interleukin-1-dependent manner. Interacts with IL-33-bound IL1RL1 to form the minimal interleukin-33 signaling complex with a 1:1:1 stoichiometry. Interacts with KIT (independently of stimulation with KITLG/SCF). A mast cell-specific KITLG/SCF-induced interleukin-33 signaling complex contains IL1RL1, IL1RAP, KIT and MYD88 (By similarity). Interacts (via the first immunoglobilin domain) with PTPRD (via the third immunoglobilin domain); induces pre- and postsynaptic differentiation of neurons (By similarity).

Family&Domains:

The TIR domain mediates NAD(+) hydrolase (NADase) activity. Self-association of TIR domains is required for NADase activity.

Belongs to the interleukin-1 receptor family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

References

1). Circ_0007611 stimulates IL-1 receptor accessory protein to inhibit trophoblast cell proliferation and induce cell apoptosis. BIOLOGY OF REPRODUCTION, 2022 (PubMed: 35238896) [IF=3.6]

Application: WB    Species: Human    Sample: placental tissues

Figure 5 Circ_0007611 mediated IL1RAP expression through miR-558. (A) The binding sites of miR-558 and IL1RAP. (B) The interaction between miR-558 and IL1RAP was identified by a dual-luciferase reporter assay. (C and D) The effects of miR-558 mimics and inhibitors on IL1RAP protein expression were determined by Western blot. (E–G) IL1RAP expression was detected by qRT-PCR and Western blot in placental tissues of PE women and normal placental tissues. (H and I) Spearman correlation analysis was implemented to analyze the correlation between IL1RAP and miR-558 or circ_0007611 expression in the placental tissues of PE women. (J and K) The effects of circ_0007611 and miR-558 on IL1RAP protein expression were testified by Western blot analysis. IL-1 receptor accessory protein (IL1RAP); wild-type (WT); mutant (MUT); 3′-untranslated region (3’UTR); IL-1 receptor accessory protein (IL1RAP); microRNA-558 (miR-558); normal pregnant women (NP); preeclampsia (PE); circRNA 0007611 (circ_0007611); GAPDH.

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