AMH Antibody - #DF13527

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Product: AMH Antibody
Catalog: DF13527
Description: Rabbit polyclonal antibody to AMH
Application: WB
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Dog
Mol.Wt.: 59kDa; 59kD(Calculated).
Uniprot: P03971
RRID: AB_2846546

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
AMH Antibody detects endogenous levels of total AMH.
RRID:
AB_2846546
Cite Format: Affinity Biosciences Cat# DF13527, RRID:AB_2846546.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Anti muellerian hormone; MIF; MIS; Muellerian inhibiting factor; Mullerian inhibiting substance;

Immunogens

Immunogen:

A synthesized peptide derived from human AMH, corresponding to a region within C-terminal amino acids.

Uniprot:

P03971(MIS_HUMAN) >>Visit HPA database.

Gene(ID):
AMH(268)
Sequence:
MRDLPLTSLALVLSALGALLGTEALRAEEPAVGTSGLIFREDLDWPPGSPQEPLCLVALGGDSNGSSSPLRVVGALSAYEQAFLGAVQRARWGPRDLATFGVCNTGDRQAALPSLRRLGAWLRDPGGQRLVVLHLEEVTWEPTPSLRFQEPPPGGAGPPELALLVLYPGPGPEVTVTRAGLPGAQSLCPSRDTRYLVLAVDRPAGAWRGSGLALTLQPRGEDSRLSTARLQALLFGDDHRCFTRMTPALLLLPRSEPAPLPAHGQLDTVPFPPPRPSAELEESPPSADPFLETLTRLVRALRVPPARASAPRLALDPDALAGFPQGLVNLSDPAALERLLDGEEPLLLLLRPTAATTGDPAPLHDPTSAPWATALARRVAAELQAAAAELRSLPGLPPATAPLLARLLALCPGGPGGLGDPLRALLLLKALQGLRVEWRGRDPRGPGRAQRSAGATAADGPCALRELSVDLRAERSVLIPETYQANNCQGVCGWPQSDRNPRYGNHVVLLLKMQVRGAALARPPCCVPTAYAGKLLISLSEERISAHHVPNMVATECGCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

References

1). Anti-Müllerian hormone regulates ovarian granulosa cell growth in PCOS rats through SMAD4. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics, 2025 (PubMed: 39865361) [IF=2.6]
2). AMH regulates ovarian granulosa cell growth in PCOS rats through SMAD4. Research Square, 2024

Application: WB    Species: Rat    Sample:

Figure 1. Expression of AMH and SMAD4 in serum, ovarian tissue and granulosa cells of PCOS rats 1A Vaginal smears (Jimsa staining 200×); (A-D) control (E-H)DHEA group; A pre-motility (B,E-H) motility C late motility D inter-motility a nucleated epithelial cells bnon-nucleated keratinised cells c leukocytes. 1B HE staining (40×) of rat ovaries; (A-C) 10, 15, 20 days oil control group; (D-F) 10, 15, 20 days DHEA group;(G-I) 10, 15, 20 days blank control group: primary follicle (PF), secondary follicle (SF), mature follicle (MF), sinus follicle (An), atretic follicle (At), corpus luteum (CL). 1C On days 10, 15, and 20 of modeling, T, AMH, and SMAD4 levels in rat serum were measured by ELISA. 1D Localisation of AMH and SMAD4 proteins in rat ovarian tissues (400×); (A,D) Oil control group (B,E)PCOS group (C,F) Blank control group; White arrows show positive expression of granulosa cells: a granulosa cells b follicular membrane cells. 1E Western blotting to detect the expression levels of AMH and SMAD4 in blank control group, oil control group and DHEA group((NC)20-day blank control group (C) oil control group (U) DHEA group). 1F Identification of rat ovarian granulosa cells: (A,B) HE staining of cells; (C-E)FSHR immunofluorescence; A 200×; B 400×; CDAPI; D FSHR; E MERGE; white arrowheads show cell nucleus. 1G (A-E)Western blotting to detect the expression of AMH, SMAD4, PCNA, BAX in granulosa cells of PCOS group and control group. F mRNA levels of SMAD4 in granulosa cells of the oil control and PCOS groups were detected by RT-qPCR. All data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.

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