Product: PFD6 Antibody
Catalog: DF13522
Description: Rabbit polyclonal antibody to PFD6
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 14kDa; 15kD(Calculated).
Uniprot: O15212
RRID: AB_2846541

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
PFD6 Antibody detects endogenous levels of total PFD6.
RRID:
AB_2846541
Cite Format: Affinity Biosciences Cat# DF13522, RRID:AB_2846541.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

H2 KE2; H2KE2; HKE2; HLA class II region expressed gene KE2; KE 2; KE2; KE2 protein; KE2, mouse, homolog of; PFD6_HUMAN; Pfdn6; Prefoldin subunit 6; Protein Ke2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAELIQKKLQGEVEKYQQLQKDLSKSMSGRQKLEAQLTENNIVKEELALLDGSNVVFKLLGPVLVKQELGEARATVGKRLDYITAEIKRYESQLRDLERQSEQQRETLAQLQQEFQRAQAAKAGAPGKA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Zebrafish
88
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15212 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K8 Ubiquitination
K15 Acetylation
K15 Ubiquitination
Y16 Phosphorylation
K21 Acetylation
K21 Ubiquitination
K25 Ubiquitination
K32 Ubiquitination
T38 Phosphorylation
K44 Ubiquitination
K58 Ubiquitination
K66 Acetylation
K66 Sumoylation
K66 Ubiquitination
Y82 Phosphorylation
T84 Phosphorylation
K88 Ubiquitination
Y90 Phosphorylation
K122 Ubiquitination
K128 Acetylation

Research Backgrounds

Function:

Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.

Subunit Structure:

Heterohexamer of two PFD-alpha type and four PFD-beta type subunits.

Family&Domains:

Belongs to the prefoldin subunit beta family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.