BIN1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Mouse kidney, using BIN1 Antibody.

Product:	BIN1 Antibody
Catalog:	DF13520
Description:	Rabbit polyclonal antibody to BIN1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Rabbit, Dog
Mol.Wt.:	64kDa; 65kD(Calculated).
Uniprot:	O00499
RRID:	AB_2846539

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(88%), Rabbit(88%), Dog(100%)

Clonality:

Polyclonal

Specificity:

BIN1 Antibody detects endogenous levels of total BIN1.

RRID:

AB_2846539
Cite Format: Affinity Biosciences Cat# DF13520, RRID:AB_2846539.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AMPH 2; AMPH2; Amphiphysin 2; Amphiphysin II; Amphiphysin like protein; amphiphysin-like; Amphiphysin-like protein; AMPHL; Bin1; BIN1_HUMAN; Box Dependant MYC Interacting Protein 1; Box-dependent myc-interacting protein 1; Bridging integrator 1; DKFZp547F068; MGC10367; MGC105358; Myc box dependent interacting protein 1; Myc box-dependent-interacting protein 1; SH3P9;

Immunogens

Immunogen:

Uniprot:

O00499(BIN1_HUMAN) >>Visit HPA database.

Gene(ID):

BIN1(274)

Expression:

O00499 BIN1_HUMAN:

Ubiquitous. Highest expression in the brain and muscle (PubMed:9182667). Expressed in oligodendrocytes (PubMed:27488240). Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter (PubMed:27488240). Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

Sequence:

O00499 BIN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLNECLQEVYEPDWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQWCQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEPAGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVPEISVTTPSQFEAPGPFSEQASLLDLDFDPLPPVTSPVKAPTPSGQSIPWDLWEPTESPAGSLPSGEPSAAEGTFAVSWPSQTAEPGPAQPAEASEVAGGTQPAAGAQEPGETAASEAASSSLPAVVVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Horse

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00499 As Substrate

O00499

Site	PTM Type	Source
A2	Acetylation	Uniprot
K28	Acetylation	Uniprot
K40	Ubiquitination	Uniprot
K53	Ubiquitination	Uniprot
R66	Methylation	Uniprot
S71	Phosphorylation	Uniprot
S79	Phosphorylation	Uniprot
K146	Ubiquitination	Uniprot
Y150	Phosphorylation	Uniprot
S152	Phosphorylation	Uniprot
K164	Ubiquitination	Uniprot
K258	Ubiquitination	Uniprot
K272	Ubiquitination	Uniprot
S276	Phosphorylation	Uniprot
K282	Ubiquitination	Uniprot
S296	Phosphorylation	Uniprot
S298	Phosphorylation	Uniprot
S303	Phosphorylation	Uniprot
T307	Phosphorylation	Uniprot
T323	Phosphorylation	Uniprot
T327	Phosphorylation	Uniprot
S331	Phosphorylation	Uniprot
S333	Phosphorylation	Uniprot
T348	Phosphorylation	Uniprot
S488	Phosphorylation	Uniprot
S489	Phosphorylation	Uniprot
T534	Phosphorylation	Uniprot

Research Backgrounds

O00499_BIN1_HUMAN

Function:

Is a key player in the control of plasma membrane curvature, membrane shaping and membrane remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production. In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation. Has actin bundling activity and stabilizes actin filaments against depolymerization in vitro.

PTMs:

Phosphorylated by protein kinase C.

Subcellular Location:

Nucleus. Cytoplasm. Endosome. Cell membrane>Sarcolemma>T-tubule.

Cytoplasm.

Tissue Specificity:

Ubiquitous. Highest expression in the brain and muscle. Expressed in oligodendrocytes. Isoform IIA is expressed only in the brain, where it is detected in the gray matter, but not in the white matter. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

Subunit Structure:

Heterodimer with AMPH (By similarity). Binds SH3GLB1 (By similarity). Interacts (via SH3 domain) with DNM1. Interacts with SYNJ1 (By similarity). Interacts (via SH3 domain) with DNM2. Isoform IIA interacts with CLTC. Isoform IIB does not interact with CLTC. Isoform IIC1 does not interact with CLTC. Isoform IIC2 does not interact with CLTC. Interacts with AP2A2. Interacts with AP2B1 (By similarity). Interacts with MYC (via N-terminal transactivation domain); the interaction requires the integrity of the conserved MYC box regions 1 and 2 (By similarity). Interacts with BIN2. Interacts with SNX4. Interacts (via BAR domain) with BACE1. Binds (via BAR domain) F-actin.

(Microbial infection) Interacts (SH3 domain) with HCV NS5A.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

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BIN1 Antibody - #DF13520