Product: PGP Antibody
Catalog: DF13507
Description: Rabbit polyclonal antibody to PGP
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 34kDa; 34kD(Calculated).
Uniprot: A6NDG6
RRID: AB_2846526

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
PGP Antibody detects endogenous levels of total PGP.
RRID:
AB_2846526
Cite Format: Affinity Biosciences Cat# DF13507, RRID:AB_2846526.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PGP; PGP_HUMAN; PGPase; Phosphoglycolate phosphatase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
A6NDG6 PGP_HUMAN:

Detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle.

Sequence:
MAAAEAGGDDARCVRLSAERAQALLADVDTLLFDCDGVLWRGETAVPGAPEALRALRARGKRLGFITNNSSKTRAAYAEKLRRLGFGGPAGPGASLEVFGTAYCTALYLRQRLAGAPAPKAYVLGSPALAAELEAVGVASVGVGPEPLQGEGPGDWLHAPLEPDVRAVVVGFDPHFSYMKLTKALRYLQQPGCLLVGTNMDNRLPLENGRFIAGTGCLVRAVEMAAQRQADIIGKPSRFIFDCVSQEYGINPERTVMVGDRLDTDILLGATCGLKTILTLTGVSTLGDVKNNQESDCVSKKKMVPDFYVDSIADLLPALQG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
86
Zebrafish
86
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - A6NDG6 As Substrate

Site PTM Type Enzyme
S71 Phosphorylation
K72 Ubiquitination
T73 Phosphorylation
K80 Ubiquitination
Y187 Phosphorylation
K235 Ubiquitination
T264 Phosphorylation
T276 Phosphorylation
T279 Phosphorylation
T285 Phosphorylation
K290 Ubiquitination
S295 Phosphorylation
S299 Phosphorylation
K300 Acetylation

Research Backgrounds

Function:

Glycerol-3-phosphate phosphatase hydrolyzing glycerol-3-phosphate into glycerol. Thereby, regulates the cellular levels of glycerol-3-phosphate a metabolic intermediate of glucose, lipid and energy metabolism. Was also shown to have a 2-phosphoglycolate phosphatase activity and a tyrosine-protein phosphatase activity. However, their physiological relevance is unclear. In vitro, has also a phosphatase activity toward ADP, ATP, GDP and GTP (By similarity).

Tissue Specificity:

Detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The highest activities occur in skeletal muscle and cardiac muscle.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family.

Research Fields

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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