Product: KCNN2 Antibody
Catalog: DF13499
Description: Rabbit polyclonal antibody to KCNN2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 63kDa; 64kD(Calculated).
Uniprot: Q9H2S1
RRID: AB_2846518

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
KCNN2 Antibody detects endogenous levels of total KCNN2.
RRID:
AB_2846518
Cite Format: Affinity Biosciences Cat# DF13499, RRID:AB_2846518.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9H2S1 KCNN2_HUMAN:

Expressed in atrial myocytes (at protein level). Widely expressed.

Sequence:
MSSCRYNGGVMRPLSNLSASRRNLHEMDSEAQPLQPPASVGGGGGASSPSAAAAAAAAVSSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIEAQMESYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS

PTMs - Q9H2S1 As Substrate

Site PTM Type Enzyme
Y6 Phosphorylation
S15 Phosphorylation
S18 Phosphorylation
T90 Phosphorylation
Y119 Phosphorylation
S135 Phosphorylation P17612 (PRKACA)
K293 Ubiquitination
K401 Ubiquitination
S464 Phosphorylation P17612 (PRKACA)
K466 Sumoylation
K484 Acetylation
T516 Phosphorylation
T531 Phosphorylation
S562 Phosphorylation
R566 Methylation
S567 Phosphorylation P17612 (PRKACA)
S568 Phosphorylation P17612 (PRKACA)
S569 Phosphorylation P17612 (PRKACA)
T574 Phosphorylation

Research Backgrounds

Function:

Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in atrial myocytes (at protein level). Widely expressed.

Subunit Structure:

Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding (By similarity).

Family&Domains:

Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily.

Research Fields

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

References

1). Roles of nucleus accumbens shell small-conductance calcium-activated potassium channels in the conditioned fear freezing. Journal of Psychiatric Research, 2023 (PubMed: 37216772) [IF=4.8]

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