KCNN2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/5

Western blot analysis of extracts from Mouse kidney, using KCNN2 Antibody.

DF13499 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P.

DF13499 at 1/100 staining Human colorectal cancer by IHC-P.

DF13499 at 1/100 staining Mouse kidney tissue by IHC-P.

DF13499 staining Hela cells by IF/ICC.

Product:	KCNN2 Antibody
Catalog:	DF13499
Description:	Rabbit polyclonal antibody to KCNN2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	63kDa; 64kD(Calculated).
Uniprot:	Q9H2S1
RRID:	AB_2846518

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

KCNN2 Antibody detects endogenous levels of total KCNN2.

RRID:

AB_2846518
Cite Format: Affinity Biosciences Cat# DF13499, RRID:AB_2846518.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Uniprot:

Q9H2S1(KCNN2_HUMAN) >>Visit HPA database.

Gene(ID):

KCNN2(3781)

Expression:

Q9H2S1 KCNN2_HUMAN:

Expressed in atrial myocytes (at protein level). Widely expressed.

Sequence:

Q9H2S1 KCNN2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSCRYNGGVMRPLSNLSASRRNLHEMDSEAQPLQPPASVGGGGGASSPSAAAAAAAAVSSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIEAQMESYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS

PTMs - Q9H2S1 As Substrate

Q9H2S1

Site	PTM Type	Enzyme	Source
Y6	Phosphorylation		Uniprot
S15	Phosphorylation		Uniprot
S18	Phosphorylation		Uniprot
T90	Phosphorylation		Uniprot
Y119	Phosphorylation		Uniprot
S135	Phosphorylation	P17612 (PRKACA)	Uniprot
K293	Ubiquitination		Uniprot
K401	Ubiquitination		Uniprot
S464	Phosphorylation	P17612 (PRKACA)	Uniprot
K466	Sumoylation		Uniprot
K484	Acetylation		Uniprot
T516	Phosphorylation		Uniprot
T531	Phosphorylation		Uniprot
S562	Phosphorylation		Uniprot
R566	Methylation		Uniprot
S567	Phosphorylation	P17612 (PRKACA)	Uniprot
S568	Phosphorylation	P17612 (PRKACA)	Uniprot
S569	Phosphorylation	P17612 (PRKACA)	Uniprot
T574	Phosphorylation		Uniprot

Research Backgrounds

Q9H2S1_KCNN2_HUMAN

Function:

Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in atrial myocytes (at protein level). Widely expressed.

Subunit Structure:

Heterooligomer. The complex is composed of 4 channel subunits each of which binds to a calmodulin subunit which regulates the channel activity through calcium-binding (By similarity).

Family&Domains:

Belongs to the potassium channel KCNN family. KCa2.2/KCNN2 subfamily.

Research Fields

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Endocrine system > Insulin secretion. (View pathway)

References

1). Roles of nucleus accumbens shell small-conductance calcium-activated potassium channels in the conditioned fear freezing. Journal of Psychiatric Research, 2023 (PubMed: 37216772) [IF=4.8]

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KCNN2 Antibody - #DF13499